Crosslinked poly(vinyl alcohol) supports for the immobilization of a lipolytic enzyme

Abstract

Different esters of crosslinked poly(vinyl alcohol) (PVA) were synthetized. They were developed for protein fractionation and immobilization. PVA was crosslinked with epichlorohydrin (CL-PVA) and esterified with linear fatty acids of different length (Cn-CL-PVA). A characterization of the obtained products was made. The swelling behavior, the solubility, and the percentage of esterification were examined. Values of equilibrium water content of about 81% were reached for CL-PVA samples. The polymers' stability and morpholgy were also investigated. Thermal analysis showed an increase in matrices stability, while SEM data showed the superficial development due to crosslinking and esterification reactions. Moreover, evident morphological inhomogeneities, mainly in the commercial and crosslinked products rather than in the final polymer, were present. Finally, immobilization experiments with a commercial crude of Candida rugosa were performed. Experiments showed a greater affinity of the protein for carbon chain length ranging from 8 to 12. Data indicated that compared to Celite 545, C8-CL-PVA was a better support for enzyme immobilization by physical adsorption, confirming the fact that microbial lipases prefer hydrophobic supports.

Surface Properties of Poly(vinyl alcohol) Films Dominated by Spontaneous Adsorption of Ethanol and Governed by Hydrogen Bonding

“…18−21 Many heuristic studies have been undertaken aimed at probing adsorption-induced polymer surface structural changes at the molecular level, including the ordered alignment of liquid crystal molecules on rubbed polymer substrate surfaces, 22 formation of surface hydrogen bonds of poly(2-methoxyethyl acrylate) with water and bisphenol A, 5,23 and adsorption-induced orientational order changes of the phenyl groups at phenolic resin surfaces. 24 Because of their excellent biocompatibility, biodegradability, and water solubility properties, polyvinyl alcohol (PVA)-based materials have received considerable attention for application in many areas, such as environmentally safe products, 25 protein purification, 26 enzyme immobilization, 27 membrane separation, 28,29 and biomechanical applications. 30 In most of the applications above, spontaneous adsorption of various molecules is common on PVA surfaces, resulting in poor performance of the PVA materials.…”

“…Because of their excellent biocompatibility, biodegradability, and water solubility properties, polyvinyl alcohol (PVA)-based materials have received considerable attention for application in many areas, such as environmentally safe products, protein purification, enzyme immobilization, membrane separation, , and biomechanical applications . In most of the applications above, spontaneous adsorption of various molecules is common on PVA surfaces, resulting in poor performance of the PVA materials.…”

Electrospun poly(vinyl alcohol)/glucose oxidase biocomposite membranes for biosensor applications

“…Besides, it is hydrophilic, it can be easily modified through its hydroxylic groups, and it is commercially available in a wide range of molecular weights at a low price. Therefore it is used in various cross-linked forms giving hydrogels or membranes to employ in protein purification and immobilization studies [6]. Several studies have been reported on the use of PVA for enzyme immobilization [7].…”

Thermal properties and morphology of a poly(vinyl alcohol)/silica nanocomposite prepared with a self‐assembled monolayer technique

“…Poly(vinyl alcohol) (PVA), having water‐soluble, biodegradable, biocompatible, gas‐barrier, and high mechanical properties, is attractive for environmentally friendly products, medical applications, protein purification, enzyme immobilization, and membrane preparation 6–14. More recently, PVA‐based nanocomposites have been developed to improve PVA's properties and further explore its applications 15–17.…”