Crosslinking of extracellular matrix proteins: A preliminary report on a possible mechanism of argon laser welding

Murray, Louann W.; Su, Lyndon D.; Kopchok, George E.; White, Rodney A.

doi:10.1002/lsm.1900090512

Cited by 89 publications

(30 citation statements)

References 13 publications

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“…It has been recognized that they are more thermally unstable than fibrillar collagen [49][50][51] and supposedly undergo thermal modifications at lower temperatures. Conventional processing for TEM analysis is very useful for studying collagen fibrils in connective tissue, but is unsuitable for detecting these macromolecules.…”

Section: Discussionmentioning

confidence: 99%

Microscopic characterization of collagen modifications induced by low‐temperature diode‐laser welding of corneal tissue

Matteini

Rossi

Menabuoni³

et al. 2007

Lasers Surg Med

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The preservation of substantially intact, undenatured collagen fibrils in laser-welded corneal wounds supported the thermodynamic studies that we carried out recently, which indicated temperatures below 66 degrees C at the weld site under laser irradiation. This observation enabled us to hypothesize that the mechanism, proposed in the literature, of unwinding of collagen triple helixes followed by fibrils "interdigitation" is not likely to occur in the welding process that we set up for the corneal suturing.

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Section: Discussionmentioning

confidence: 99%

Microscopic characterization of collagen modifications induced by low‐temperature diode‐laser welding of corneal tissue

Matteini

Rossi

Menabuoni³

et al. 2007

Lasers Surg Med

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“…In a SDS-PAGE study of non-pure collagen tissue, Bass et al [8] reported no changes in band distribution after laser irradiation. In contradiction to these findings, Murray et al [10] reported the appearance of a new band in the SDS-PAGE of argon-laser-welded guinea pig skin. This band had to result from a newly formed high-molecular-weight protein, enforcing the hypothesis on covalent bonding as the mechanical basis of laser welding and soldering.…”

Section: Discussionmentioning

confidence: 90%

“…On the other hand, laser tissue welding and soldering with high cellular/matrix ratio in tissues like skin, blood vessels, intestinal submucosa and kidneys demonstrated a superior tensile strength upon laser tissue welding [4,5,9,10,[15][16][17][18][19][20]. These observations raise the question whether intracellular cytoplasm components might be the one delivering the necessary "ingredients" for tensile strength induction in thermal tissue connection.…”

Section: -D Conformation Changes Of Collagen Moleculesmentioning

confidence: 99%

“…The studies analyzing the covalent forces after laser irradiation using the sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique were performed with collagen-rich substrates containing more or less cellular components. The reported results have been so far controversial [8,10,11], most likely due to the simultaneous use of protein solder and complex multicellular tissues. The aim of the present study was to analyze the impact of laser irradiation on 100% pure cell-free collagen molecules, using ICG for light absorption without albumin solder.…”

Section: Introductionmentioning

confidence: 99%

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Effect of laser soldering irradiation on covalent bonds of pure collagen

et al. 2006

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Laser tissue welding and soldering is being increasingly used in the clinical setting for defined surgical procedures. The exact induced changes responsible for tensile strength are not yet fully investigated. To further improve the strength of the bonding, a better understanding of the laser impact at the subcellular level is necessary. The goal of this study was to analyze whether the effect of laser irradiation on covalent bonding in pure collagen using irradiances typically applied for tissue soldering. Pure rabbit and equine type I collagen were subjected to laser irradiation. In the first part of the study, rabbit and equine collagen were compared using identical laser and irradiation settings. In the second part of the study, equine collagen was irradiated at increasing laser powers. Changes in covalent bonding were studied indirectly using the sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) technique. Tensile strengths of soldered membranes were measured with a calibrated tensile force gauge. In the first experiment, no differences between the species-specific collagen bands were noted, and no changes in banding were found on SDS-PAGE after laser irradiation. In the second experiment, increasing laser irradiation power showed no effect on collagen banding in SDS-PAGE. Finally, the laser tissue soldering of pure collagen membranes showed virtually no determinable tensile strength. Laser irradiation of pure collagen at typical power settings and exposure times generally used in laser tissue soldering does not induce covalent bonding between collagen molecules. This is true for both rabbit and equine collagen proveniences. Furthermore, soldering of pure collagen membranes without additional cellular components does not achieve the typical tensile strength reported in native, cell-rich tissues. This study is a first step in a better understanding of laser impact at the molecular level and might prove useful in engineering of combined collagen-soldering matrix membranes for special laser soldering applications.

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“…During welding, water in the tissue absorbs the laser energy and subsequently heats the collagen helix. When the collagen tissue temperature raises above 608C, bonding is disrupted and partial dissociation occurs, followed by covalent [47] and/or non-covalent [48,49] bonding of the tissue protein molecules as the tissue cools. Successful welding therefore requires precise control of laser power and exposure times to control tissue temperature and dehydration.…”

Section: Introductionmentioning

confidence: 99%

NIR laser tissue welding of in vitro porcine cornea and sclera tissue

et al. 2004

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We conclude that an NIR laser system using an optimal laser radiation wavelength of 1,455 nm can effectively weld cornea and sclera tissue and that this laser tissue welding (LTW) methodology typically causes minimal disruption of tissue, and thus, avoids opacities and irregularities in the tissue which may result in decreased visual acuity. The optimization of a laser welding system that leads to a strong full thickness tissue bond without tissue destruction, an instant seal that promotes wound healing, and the absence of a continued presence of a foreign substance like a suture, is of considerable importance to the ophthalmology medical community. This need is especially apparent with respect to corneal transplantation and fixing the position of corneal flaps in Laser-Assisted In Situ Keratomileusis (LASIK), a laser procedure used to permanently change the shape of the cornea.

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Crosslinking of extracellular matrix proteins: A preliminary report on a possible mechanism of argon laser welding

Cited by 89 publications

References 13 publications

Microscopic characterization of collagen modifications induced by low‐temperature diode‐laser welding of corneal tissue

Microscopic characterization of collagen modifications induced by low‐temperature diode‐laser welding of corneal tissue

Effect of laser soldering irradiation on covalent bonds of pure collagen

NIR laser tissue welding of in vitro porcine cornea and sclera tissue

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