Crossover behavior in stress relaxations of poroelastic and viscoelastic dominant hydrogels

Li, Hangyu; Lian, Xiaozheng; Guan, Dongshi

doi:10.1039/d3sm00592e

Cited by 3 publications

(2 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The colloidal probe consists of a glass sphere of radius R (≅ 5 µm), which is glued on the front end of a rectangular cantilever beam (NSC35/Pt, MikroMasch) featuring a spring constant of 0.2 N/m. The AFM measurements were carried out in contact mode and a constant loading speed v = 10 µm/s and the measured force-distance curves were recorded and tted with Hertz model E to calculate the Young's modulus E 30 . All the force measurements were made at a temperature ∼37 •C, which is monitored and maintained by a local temperature control inside the uid chamber.…”

Section: Atomic Force Microscope (Afm)mentioning

confidence: 99%

TRPML1 ion channel promote HepaRG cells differentiation under simulated microgravity conditions

Gao,

Fan,

Lü

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

Stem cell differentiation must be regulated by intricate and complex interactions between cells and their surrounding environment, ensuring normal organ and tissue morphology such as the liver 1. Though it is well acknowledged that microgravity provides necessary mechanical force signals for cells fate 2, how microgravity affects growth, differentiation, and communication is still largely unknown due to the lack of real experimental scenarios and reproducibility tools. Here, we used Rotating Flat Chamber (RFC) to simulate ground-based microgravity effects to study how microgravity affects the differentiation of HepaRG (hepatic progenitor cells) cells. Unexpectedly, we found that simulated microgravity could promote HepaRG cells differentiation which was exhibited as increased expression of Alpha-fetoprotein (AFP), albumin (ALB), and Recombinant Cytokeratin 18 (CK18). Through screening a series of mechanical receptors, we identified that ion channel TRPML1 was critical for the differentiation promotion effect of microgravity. Once TRPML1 was activated by microgravity, the concentration of lysosomal calcium ions was increased to activate the Wnt/β-catenin signaling pathway, which finally led to the enhanced cell differentiation of HepaRG cells. In addition, the cytoskeleton was remodeled under microgravity conditions and then influenced the expression of PI (3,5) P2, which is the best-known activator of TRPML1. In summary, our findings have established a mechanism by which microgravity promotes the differentiation of HepaRG cells through the TRPML1 signaling pathway, which may provide a potential target for the regulation of hepatic stem/progenitor cells differentiation and embryonic liver development under microgravity conditions.

show abstract

Section: Atomic Force Microscope (Afm)mentioning

confidence: 99%

TRPML1 ion channel promote HepaRG cells differentiation under simulated microgravity conditions

Gao,

Fan,

Lü

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Islam and Oyen examined how different models of time-dependent behavior fit cell-scale relaxation responses to classify the behavior as having more poroelastic or viscoelastic character [34]. Other researchers have used a similar fitting approach to extract time points where the dominant time-dependent behavior changes [36]. While useful, these approaches do not permit the true isolation of the contributions of poroelasticity and viscoelasticity in soft hydrated materials.…”

Section: Introductionmentioning

confidence: 99%

Isolating Poroelastic and Viscoelastic Mechanisms of Soft Tissues and Hydrogels Through Sequential Microscale Indentation Testing: New Applications of Indentation Theory for Microscale Characterization

Zahin,

Al Barghouthi,

Dickerson

2024

Preprint

View full text Add to dashboard Cite

Soft hydrated materials, including biological tissues and hydrogels, exhibit complex time-dependent mechanical behaviors due to their poroelastic and viscoelastic properties. These properties often manifest on overlapping time scales, making it challenging to isolate the individual contributions of poroelasticity and viscoelasticity to the overall mechanical response. This study presents a novel semi-analytical model for characterizing these properties through sequential microscale load relaxation indentation testing. By extending existing theories, we developed a poroviscoelastic framework that enables the deconvolution of poroelastic and viscoelastic effects. Using this model to fit sequential microscale indentation data, we characterized porcine heart and liver tissues, as well as collagen and GelMA hydrogels, revealing distinct differences in their poroelastic and viscoelastic parameters. Our findings demonstrate that this approach not only provides rapid and detailed insights into the mechanical properties at the microscale but also offers significant advantages over traditional methods in terms of speed, computational efficiency, and practicality. This methodology has broad implications for advancing the understanding of tissue mechanics and the design of biomimetic materials for tissue engineering applications.Statement of SignificanceThis study introduces a novel approach to understanding the mechanical behavior of soft hydrated materials, like tissues and hydrogels. This study introduces a semi-analytical model to describe the time dependent behavior and a practical approach to distinguish between poroelasticity and viscoelasticity at the microscale. By providing this model along with a rapid and efficient characterization method, our approach enhances understanding of time-dependent mechanical behaviors critical for soft tissue mechanics and biomaterials design.

show abstract

Gelatin-Based Scaffolds with Carrageenan and Chitosan for Soft Tissue Regeneration

Pasini,

Re,

Trenta

et al. 2024

Gels

View full text Add to dashboard Cite

Motivated by the enormous potential of hydrogels in regenerative medicine, new biocompatible gelatin-based hybrid hydrogels were developed through a green process using poly(ethylene glycol) diglycidyl ether as a cross-linking agent, adding carrageenan and chitosan polysaccharides to the network to better mimic the hybrid composition of native extracellular matrix. Overall, the hydrogels show suitable structural stability, high porosity and pore interconnectivity, good swellability, and finally, biocompatibility. Their mechanical behavior, investigated by tensile and compression tests, appears to be characterized by nonlinear elasticity with high compliance values, fast stress-relaxation, and good strain reversibility with no sign of mechanical failure for compressive loading–unloading cycles at relatively high deformation levels of 50%. Degradation tests confirm the hydrogel bioresorbability by gradual hydrolysis, during which the structural integrity of both materials is maintained, while their mechanical behavior becomes more and more compliant. Human Umbilical Cord-derived Mesenchymal Stem Cells (hUC-MSCs) were used to test the hydrogels as potential carriers for cell delivery in tissue engineering. hUC-MSCs cultured inside the hydrogels show a homogenous distribution and maintain their growth and viability for at least 21 days of culture, with an increasing proliferation trend. Hence, this study contributes to a further understanding of the potential use of hybrid hydrogels and hUC-MSCs for a wide range of biomedical applications, particularly in soft tissue engineering.

show abstract

Crossover behavior in stress relaxations of poroelastic and viscoelastic dominant hydrogels

Abstract: The mechanical response and relaxation behavior of hydrogels are crucial to their diverse functions and applications. However, understanding how stress relaxation depends on the material properties of hydrogels and accurately...

Cited by 3 publications

References 49 publications

TRPML1 ion channel promote HepaRG cells differentiation under simulated microgravity conditions

TRPML1 ion channel promote HepaRG cells differentiation under simulated microgravity conditions

Isolating Poroelastic and Viscoelastic Mechanisms of Soft Tissues and Hydrogels Through Sequential Microscale Indentation Testing: New Applications of Indentation Theory for Microscale Characterization

Gelatin-Based Scaffolds with Carrageenan and Chitosan for Soft Tissue Regeneration

Contact Info

Product

Resources

About