In this article, an analytical method is presented to precisely estimate the crosstalk of uniform and nonuniform microstrip-coupled transmission lines (TLs) in frequency domain using modified transmission matrix. The obtained expression is quantitatively related in terms of the geometrical parameters of the coupled lines. A straightforward procedure is presented to obtain a closed form formula to accurately determine the crosstalk of a microstrip-coupled line. For a nonuniform structure, the TL is divided into a few series of uniform sections with a specific modified transmission matrix. The total modified transmission matrix is determined by multiplying the modified transmission matrix of different sections and then the crosstalk can be calculated using the total modified transmission matrix. Several structures are considered to confirm the validity of the presented method. It is shown that the obtained results are in a good agreement with those obtained by simulation and measurement. K E Y W O R D S crosstalk, transmission line (TL), transmission matrix