Crosstalk and trade-offs: Plant responses to climate change-associated abiotic and biotic stresses

Leisner, Courtney P.; Potnis, Neha; Sanz‐Sáez, Álvaro

doi:10.22541/au.166462027.71310652/v1

Cited by 6 publications

(1 citation statement)

References 177 publications

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“…Increasing crop production and enhancing plant health in a sustainable manner is critical in the face of climate change (Farooq et al, 2022), increasing population (Komarek and Msangi, 2019), reduction of cultivable lands (Maranguit et al, 2017), and pest or pathogen mediated diseases in crops (Leisner et al, 2022). While chemical fertilizers and pesticides remain necessary for enhancing food production, their indiscriminate use has proven unsustainable and negatively impacts human and environmental health (Chaudhary et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Screening the maize rhizobiome for consortia that improve Azospirillum brasilense root colonization and plant growth outcomes

Barua

Clouse

Diaz

et al. 2023

Front. Sustain. Food Syst.

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Plant growth-promoting bacteria (PGPB) are valuable for supporting sustainable food production and may alleviate the negative impacts of chemical fertilizers on human health and the environment. While single-strain inoculations have proven unreliable due to poor survival and colonization in the rhizosphere, application of PGPB in multispecies consortia has the potential to improve these outcomes. Here, we describe a new approach for screening and identifying bacterial consortia that improve the growth of corn relative to plants inoculated with a single strain. The method uses the microwell recovery array (MRA), a microfabricated high-throughput screening device, to rapidly explore the maize (Zea mays L.) rhizobiome for higher-order combinations of bacteria that promote the growth and colonization of the nitrogen-fixing PGPB, Azospirillum brasilense. The device simultaneously generates thousands of random, unique combinations of bacteria that include A. brasilense and members of the maize rhizobiome, then tracks A. brasilense growth in each combination during co-culture. Bacteria that show the highest levels of A. brasilense growth promotion are then recovered from the device using a patterned light extraction technique and are identified. With this approach, the screen uncovered growth-promoting consortia consisting primarily of bacteria from the Acinetobacter-Enterobacter-Serratia genera, which were then co-inoculated with A. brasilense on axenic maize seedlings that were monitored inside a plant growth chamber. Compared to maize plants inoculated with A. brasilense alone, plants that were co-inoculated with these consortia showed accelerated growth after 15 days. Follow-up root colonization assays revealed that A. brasilense colonized at higher levels on roots from the co-inoculated seedlings. These findings demonstrate a new method for rapid bioprospecting of root and soil communities for complementary PGPB and for developing multispecies consortia with potential use as next-generation biofertilizers.

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Section: Introductionmentioning

confidence: 99%

Screening the maize rhizobiome for consortia that improve Azospirillum brasilense root colonization and plant growth outcomes

Barua

Clouse

Diaz

et al. 2023

Front. Sustain. Food Syst.

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Xanthomonas infection and ozone stress distinctly influence the microbial community structure and interactions in the pepper phyllosphere

Bhandari

Sanz‐Sáez

Leisner

et al. 2023

ISME Communications

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While the physiological and transcriptional response of the host to biotic and abiotic stresses have been intensely studied, little is known about the resilience of associated microbiomes and their contribution towards tolerance or response to these stresses. We evaluated the impact of elevated tropospheric ozone (O3), individually and in combination with Xanthomonas perforans infection, under open-top chamber field conditions on overall disease outcome on resistant and susceptible pepper cultivars, and their associated microbiome structure, function, and interaction network across the growing season. Pathogen infection resulted in a distinct microbial community structure and functions on the susceptible cultivar, while concurrent O3 stress did not further alter the community structure, and function. However, O3 stress exacerbated the disease severity on resistant cultivar. This altered diseased severity was accompanied by enhanced heterogeneity in associated Xanthomonas population counts, although no significant shift in overall microbiota density, microbial community structure, and function was evident. Microbial co-occurrence networks under simultaneous O3 stress and pathogen challenge indicated a shift in the most influential taxa and a less connected network, which may reflect the altered stability of interactions among community members. Increased disease severity on resistant cultivar may be explained by such altered microbial co-occurrence network, indicating the altered microbiome-associated prophylactic shield against pathogens under elevated O3. Our findings demonstrate that microbial communities respond distinctly to individual and simultaneous stressors, in this case, O3 stress and pathogen infection, and can play a significant role in predicting how plant-pathogen interactions would change in the face of climate change.

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Regulating temperature and humidity inhibits the disease severity of tomato

Li,

2023

Preprint

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The environment significantly impacts the interactions between plants and pathogens, thus remarkably affecting crop disease occurrence. Regulating the environmental conditions in greenhouses to inhibit plant diseases is conducive to the development of green ecological agriculture. However, how to specific regulate the temperature and humidity to reduce plant diseases remain unclear. In this study, different methods of temperature and humidity regulation were performed. Histological observation and dual RNA-seq analysis showed that increasing temperature and decreasing humidity at 24 h post inoculation (hpi) for 12 h could effectively inhibit disease severity of tomatoes. The regulation induced the expression of heat shock proteins (HSPs) and defense-related genes of tomatoes in response to B. cinerea. Bcl-2-associated athanogenes (BAGs) may be involved in tomato resistance to B. cinerea mediated by temperature and humidity regulation. In addition, the infection process and the expression of toxin-related genes of B. cinerea such as sesquiterpene botrydial (BOT) and polyketide botcinic acid (BOA) was inhibited. Overall, we obtained the optimal regulation method of temperature and humidity to alleviate tomato gray mold, and preliminarily explored its mechanisms of inhibiting the disease.

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Crosstalk and trade-offs: Plant responses to climate change-associated abiotic and biotic stresses

Cited by 6 publications

References 177 publications

Screening the maize rhizobiome for consortia that improve Azospirillum brasilense root colonization and plant growth outcomes

Screening the maize rhizobiome for consortia that improve Azospirillum brasilense root colonization and plant growth outcomes

Xanthomonas infection and ozone stress distinctly influence the microbial community structure and interactions in the pepper phyllosphere

Regulating temperature and humidity inhibits the disease severity of tomato

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