Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity

Zhang, Yi; Zeng, Lirong

doi:10.1016/j.xplc.2020.100041

Cited by 71 publications

(42 citation statements)

References 210 publications

(179 reference statements)

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“…Yeast Sir2 also displayed this dual enzyme activity (19). There is a great possibility of crosstalk between different types of these modifications, say ADPr with acetylation (17) or ubiquitination with other PTM (20).…”

Section: Introductionmentioning

confidence: 99%

Understanding the mechanism of monoADP ribosylation in OsSRT1 and its linkage to DNA repair system under stress conditions

Mitra

Dey

2021

Preprint

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The role of sirtuins in plants are slowly unraveling. There are only reports of H3K9Ac deacetylation by OsSRT1. Here our studies shade light on its dual enzyme capability with preference for mono ADP ribosylation over deacetylation. OsSRT1 can specifically transfer the single ADP ribose group on its substrates in an enzymatic manner. This mono ADPr effect is not well known in plants, more so for deacetylases. The products of this reaction (NAM and ADP ribose) have immense negative effect on this enzyme suggesting a tighter regulation. Resveratrol, a natural plant polyphenol proves to be a strong activator of this enzyme at 150 μM concentration. Under different abiotic stress conditions, we could link this ADP ribosylase activity to the DNA repair pathway by activating the enzyme PARP1. Metal stress in plants also influences these enzyme activities.

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Section: Introductionmentioning

confidence: 99%

Understanding the mechanism of monoADP ribosylation in OsSRT1 and its linkage to DNA repair system under stress conditions

Mitra

Dey

2021

Preprint

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“…This also provides an indication that these mutant plants are more drought-tolerant and can better limit any degradative proteolytic events. An increase in the number of proteases signifies an active defense response and their involvement in the degradation of storage proteins for the release of amino acids, which then serve as substituents in the biosynthesis of new proteins [ 97 ]. However, proteases can also be involved in the protection of crucial proteins by facilitating post-translational modification, such as in the case of SUMOylation [ 98 ].…”

Section: Discussionmentioning

confidence: 99%

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

Roux

Burger

Vlok

et al. 2021

IJMS

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Drought response in wheat is considered a highly complex process, since it is a multigenic trait; nevertheless, breeding programs are continuously searching for new wheat varieties with characteristics for drought tolerance. In a previous study, we demonstrated the effectiveness of a mutant known as RYNO3936 that could survive 14 days without water. In this study, we reveal another mutant known as BIG8-1 that can endure severe water deficit stress (21 days without water) with superior drought response characteristics. Phenotypically, the mutant plants had broader leaves, including a densely packed fibrous root architecture that was not visible in the WT parent plants. During mild (day 7) drought stress, the mutant could maintain its relative water content, chlorophyll content, maximum quantum yield of PSII (Fv/Fm) and stomatal conductance, with no phenotypic symptoms such as wilting or senescence despite a decrease in soil moisture content. It was only during moderate (day 14) and severe (day 21) water deficit stress that a decline in those variables was evident. Furthermore, the mutant plants also displayed a unique preservation of metabolic activity, which was confirmed by assessing the accumulation of free amino acids and increase of antioxidative enzymes (peroxidases and glutathione S-transferase). Proteome reshuffling was also observed, allowing slow degradation of essential proteins such as RuBisCO during water deficit stress. The LC-MS/MS data revealed a high abundance of proteins involved in energy and photosynthesis under well-watered conditions, particularly Serpin-Z2A and Z2B, SGT1 and Calnexin-like protein. However, after 21 days of water stress, the mutants expressed ABC transporter permeases and xylanase inhibitor protein, which are involved in the transport of amino acids and protecting cells, respectively. This study characterizes a new mutant BIG8-1 with drought-tolerant characteristics suited for breeding programs.

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“…Phosphorylation and ubiquitination are the most common protein PTMs in plants, but the regulatory relationship between ubiquitination and phosphorylation remains to be addressed. In general, phosphorylation facilitates ubiquitination, influences interactions with substrates, or controls the stability of E3 ligase (Vu et al, 2018; Zhang and Zeng, 2020). For instance, the activity of E3 ligases PUB25/26 is enhanced by phosphorylation by CALCIUM‐DEPENDENT PROTEIN KINASE 28 (CPK28), thereby promoting the degradation of BIK1 (Wang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis E3 ligase KEG associates with and ubiquitinates MKK4 and MKK5 to regulate plant immunity

Gao

Sun

Wang

et al. 2020

JIPB

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Mitogen-activated protein kinase (MAPK) cascades are highly conserved signaling modules that regulate plant immune responses. The Arabidopsis thaliana Raf-like MAPK kinase kinase ENHANCED DISEASE RESISTANCE1 (EDR1) is a key negative regulator of plant immunity that affects the protein levels of MKK4 and MKK5, two important MAPK cascade members, but the underlying mechanism is poorly understood. Here, genome-wide phosphorylation analysis demonstrated that the E3 ligase KEEP ON GOING (KEG) is phosphorylated in the edr1 mutant but not the wild type, suggesting that EDR1 negatively affects KEG phosphorylation. The identified phosphorylation sites in KEG appear to be important for its accumulation. The keg-4 mutant, a previously identified edr1 suppressor, enhances susceptibility to the powdery mildew pathogen Golovinomyces cichoracearum. In addition, MKK4 and MKK5 protein levels are reduced in the keg-4 mutant. Furthermore, we demonstrate that MKK4 and MKK5 associate with full-length KEG, but not with truncated KEG-RK or KEG-RKA, and that KEG ubiquitinates and mediates the degradation of MKK4 and MKK5. Taken together, these results indicate that MKK4 and MKK5 protein levels are regulated by KEG via ubiquitination, uncovering a mechanism by which plants finetune immune responses by regulating the homeostasis of key MAPK cascade members via ubiquitination and degradation.

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Crosstalk between Ubiquitination and Other Post-translational Protein Modifications in Plant Immunity

Cited by 71 publications

References 210 publications

Understanding the mechanism of monoADP ribosylation in OsSRT1 and its linkage to DNA repair system under stress conditions

Understanding the mechanism of monoADP ribosylation in OsSRT1 and its linkage to DNA repair system under stress conditions

EMS Derived Wheat Mutant BIG8-1 (Triticum aestivum L.)—A New Drought Tolerant Mutant Wheat Line

Arabidopsis E3 ligase KEG associates with and ubiquitinates MKK4 and MKK5 to regulate plant immunity

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