Complex interconnection structure is a common structure on printed circuit board (PCB). Herein, the paper proposes a method of crosstalk cancellation point at the crosstalk problem between microstrip lines in a complex interconnection structure. First, a model of the coupled transmission lines-channel transmission matrix (CTL-CTM) of the complex interconnection structure is established. Second, the CTL-CTM is simplified through the equivalence of crosstalk-coupling coefficient of parallel coupling microstrip lines to that of the complex interconnection structure. The eigenvalue of the simplified CTL-CTM is then decomposed, based on which the construction of crosstalk cancellation circuit is performed. Simulation results show that the proposed method can effectively improve the quality of eye patterns on complex interconnection structures.