Crosstalk in the non-classical signal transduction of testosterone and retinol in immature rat testes

Nascimento, Monica Andressa Wessner do; Cavalari, Fernanda Carvalho; Oliveria, Vanessa Staldoni de; Gonçalves, Renata; Menegaz, Danusa; Loss, Eloisa da Silveira; Silva, Fátima Regina Mena Barreto

doi:10.1016/j.steroids.2019.108522

Cited by 4 publications

(2 citation statements)

References 36 publications

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“…Sertoli cells present a plasma membrane microenvironment that contains, among the different classes of lipids, proteins that function as ionic channels [1,2]. The kinetics of the ionic channels of Sertoli cells coordinate rapid response events, such as the control of plasma membrane potential, by influencing Na + -K + -ATPase [3][4][5][6], the activities of enzymes that are located at the plasma membrane [4], cell secretion as well as genomic responses as nuclear transcription [7][8][9]. Accordingly, the rapid response activities of the Na + -K + -ATPase pump, calcium-dependent potassium channels (Ca 2+ -K + ), ATP-dependent potassium channels (ATP-K + ), and voltage-sensitive potassium currents (Kv) support the function of Sertoli cells to maintain spermatogenesis and fertility.…”

Section: Introductionmentioning

confidence: 99%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques,

Gamba,

Menegaz

et al. 2023

Biomed. Phys. Eng. Express

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Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses by involving ionic channels.The rapid responses of Sertoli cells in culture were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools (from intact seminiferous tubules). A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K+ pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells in the intact seminiferous tubules and of Sertoli cells in culture (patch clamp measurements).The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K+ pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells.Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of Sertoli cells to hormonal stimuli.

show abstract

Section: Introductionmentioning

confidence: 99%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques,

Gamba,

Menegaz

et al. 2023

Biomed. Phys. Eng. Express

Self Cite

View full text Add to dashboard Cite

show abstract

“…Sertoli cells present a plasma membrane microenvironment that contains, among the different classes of lipids, proteins that function as ionic channels 1,2 . The kinetics of the ionic channels of Sertoli cells coordinate events, such as the control of plasma membrane potential, by influencing Na + -K + -ATPase [3][4][5][6] , the activities of enzymes that are located at the plasma membrane 4 , cell secretion and nuclear transcription [7][8][9] . Accordingly, the activities of the Na + -K + -ATPase pump, calcium-dependent potassium channels (Ca 2+ -K + ), ATP-dependent potassium channels (ATP-K + ), and voltage-sensitive potassium currents (Kv) support the function of Sertoli cells to maintain spermatogenesis and fertility.…”

Section: Introductionmentioning

confidence: 99%

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Taques

Gamba

Menegaz

et al. 2022

Preprint

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Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses. The responses of Sertoli cells in culture to hormone stimulation were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools. A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na+/K + pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells collected from intact seminiferous tubules and of Sertoli cells in culture. The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na+/K + pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells. Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of non-excitable cells to hormonal stimuli.

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Estradiol and 1α,25(OH)2 vitamin D3 share plasma membrane downstream signal transduction through calcium influx and genomic activation in immature rat testis

et al. 2021

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Crosstalk in the non-classical signal transduction of testosterone and retinol in immature rat testes

Cited by 4 publications

References 36 publications

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches

Estradiol and 1α,25(OH)2 vitamin D3 share plasma membrane downstream signal transduction through calcium influx and genomic activation in immature rat testis

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