Crosstalk of mesenchymal stem cells and macrophages promotes cardiac muscle repair

Wang, Mei; Zhang, Guoru; Wang, Yaling; Liu, Tao; Zhang, Yang; An, Yu; Li, Yongjun

doi:10.1016/j.biocel.2014.11.003

Cited by 34 publications

(22 citation statements)

References 27 publications

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“…In another report, mesenchymal stem cells transplanted to injured heart not only recruit macrophages to enhance angiogenesis to promote regeneration of cardiac muscle, but also secrete BMP7 to contradict the fibrogenic effect of TGFβ1 by macrophages [23]. Both studies highlighted that M2 macrophages secreted high TGFβ1, which was confirmed in the current study.…”

Section: Discussionsupporting

confidence: 78%

“…A recent study showed that M2 macrophages were recruited to the injured pancreas, and produced and secreted high levels of transforming growth factor β1 (TGFβ1) [22]. In another report, mesenchymal stem cells were transplanted to cure cardiac muscle injury, while the authors found that the MSCs not only recruit macrophages to enhance angiogenesis to promote regeneration of cardiac muscle, but also secrete BMP7 to contradict the fibrogenic effect of TGFβ1 by macrophages [23]. Interestingly, another several studies have demonstrated a role of PLGF in the crosstalk between tumor cells and macrophages [15,24].…”

Section: Introductionmentioning

confidence: 99%

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Placental Growth Factor Mediates Crosstalk Between Lung Cancer Cells and Tumor-Associated Macrophages in Controlling Cancer Vascularization and Growth

Zhu

Bai

et al. 2018

Cell Physiol Biochem

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Background/Aims: Assistance with tumor-associated vascularization is needed for the growth and invasion of non-small cell lung cancer (NSCLC). Recently, it was shown that placental growth factor (PLGF) expressed by NSCLC cells had a critical role in promoting the metastasis of NSCLC cells. However, the underlying molecular mechanisms remain elusive. Methods: Here, we first established a NSCLC model in mice that allows us not only to isolate tumor cells from non-tumor cells in the tumor, but also to trace tumor cells in living animals. Levels of PLGF, its unique receptor Flt-1, as well as transforming growth factor β1 (TGFβ1) was examined in tumor cells and tumor-associated macrophages (TAM) by RT-qPCR. A transwell well co-culture system and HUVEC assay were applied to study the crosstalk between NSCLC cells and TAM. Results: NSCLC cells produced and secreted PLGF to signal to tumor-associated macrophages (TAM) through surface expression of Flt-1 on macrophages. In a transwell co-culture system, PLGF secreted by NSCLC cells triggered macrophage polarization to a TAM subtype that promote growth of NSCLC cells. Moreover, polarized TAM seemed to secrete TGFβ1 to enhance the growth of endothelial cells in a HUVEC assay. Conclusion: The cross-talk between TAM and NSCLC cells via PLGF/Flt-1 and TGFβ receptor signaling may promote the growth and vascularization of NSCLC.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Placental Growth Factor Mediates Crosstalk Between Lung Cancer Cells and Tumor-Associated Macrophages in Controlling Cancer Vascularization and Growth

Zhu

Bai

et al. 2018

Cell Physiol Biochem

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“…Berry et al 12 reported that new cardiomyocytes formed in the dystrophic heart and that nestin-expressing interstitial cells could generate them in addition to other cells of the cardiac lineage. Wang et al 13 showed that transplantation of mesenchymal stem cells facilitated cardiac muscle repair.…”

Section: Resultsmentioning

confidence: 99%

From hair to heart: nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells differentiate to beating cardiac muscle cells

et al. 2015

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We have previously demonstrated that the neural stem-cell marker nestin is expressed in hair follicle stem cells located in the bulge area which are termed hair-follicle-associated pluripotent (HAP) stem cells. HAP stem cells from mouse and human could form spheres in culture, termed hair spheres, which are keratin 15-negative and CD34-positive and could differentiate to neurons, glia, keratinocytes, smooth muscle cells, and melanocytes in vitro. Subsequently, we demonstrated that nestin-expressing stem cells could effect nerve and spinal cord regeneration in mouse models. In the present study, we demonstrated that HAP stem cells differentiated to beating cardiac muscle cells. We separated the mouse vibrissa hair follicle into 3 parts (upper, middle, and lower), and suspended each part separately in DMEM containing 10% FBS. All three parts of hair follicle differentiated to beating cardiac muscle cells as well as neurons, glial cells, keratinocytes and smooth muscle cells. The differentiation potential to cardiac muscle is greatest in the upper part of the follicle. The beat rate of the cardiac muscle cells was stimulated by isoproterenol and inhibited by propanolol. HAP stem cells have potential for regenerative medicine for heart disease as well as nerve and spinal cord repair.

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“…Wnt) [180] or vascular (e.g. VEGF) [181] pathways, a growing number of reports involve modulating inflammation to improve tissue [90, 182, 183] and bone repair by biomimetic approaches [70, 94, 96]. In this line, M1 and M2 macrophages have been recently described as promoters of scaffold vascularization in vitro by promoting the sprout formation by endothelial cells and the anastomosis of M1-induced sprouting vessels, respectively [183].…”

Section: Opportunities For Enhancing Bone Repair By Modulating Infmentioning

confidence: 99%

Inflammation, fracture and bone repair

Loi

Córdova

Pajarinen

et al. 2016

Bone

972

883

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The reconstitution of lost bone is a subject that is germane to many orthopaedic conditions including fractures and non-unions, infection, inflammatory arthritis, osteoporosis, osteonecrosis, metabolic bone disease, tumors, and periprosthetic particle-associated osteolysis. In this regard, the processes of acute and chronic inflammation play an integral role. Acute inflammation is initiated by endogenous or exogenous adverse stimuli, and can become chronic in nature if not resolved by normal homeostatic mechanisms. Dysregulated inflammation leads to increased bone resorption and suppressed bone formation. Crosstalk amongst inflammatory cells (polymorphonuclear leukocytes and cells of the monocyte-macrophage-osteoclast lineage) and cells related to bone healing (cells of the mesenchymal stem cell-osteoblast lineage and vascular lineage) is essential to the formation, repair and remodeling of bone. In this review, the authors provide a comprehensive summary of the literature related to inflammation and bone repair. Special emphasis is placed on the underlying cellular and molecular mechanisms, and potential interventions that can favorably modulate the outcome of clinical conditions that involve bone repair.

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Crosstalk of mesenchymal stem cells and macrophages promotes cardiac muscle repair

Cited by 34 publications

References 27 publications

Placental Growth Factor Mediates Crosstalk Between Lung Cancer Cells and Tumor-Associated Macrophages in Controlling Cancer Vascularization and Growth

Placental Growth Factor Mediates Crosstalk Between Lung Cancer Cells and Tumor-Associated Macrophages in Controlling Cancer Vascularization and Growth

From hair to heart: nestin-expressing hair-follicle-associated pluripotent (HAP) stem cells differentiate to beating cardiac muscle cells

Inflammation, fracture and bone repair

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