Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients

Karema-Jokinen, Viivi I.; Koskela, Ali; Hytti, Maria; Hongisto, Heidi; Viheriälä, Taina; Liukkonen, Mikko; Torsti, Tommi; Skottman, Heli; Kauppinen, Anu; Nymark, Soile

doi:10.1016/j.jbc.2023.104770

Cited by 8 publications

(2 citation statements)

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“…Therefore, specific LTCCs antagonists need to be developed in future. Furthermore, the LTCCs were new promising targets for many diseases, such as drug-addiction [ 33 , 34 ], depressive disorder [ 109 ], age-related macular degeneration-retinal pigment epithelium (AMD-RPE) [ 110 ], intrauterine growth restriction [ 111 ], local infiltration analgesia [ 112 ], myalgia [ 113 ], which expanded the scope of application. In addition, calcium channel blocker (CCB) have a short plasma half-life especially in rodents and show high first-pass metabolism upon oral application [ 114 ].…”

Section: Discussionmentioning

confidence: 99%

Natural L-type calcium channels antagonists from Chinese medicine

Xu,

Cai,

Liu

et al. 2024

Chin Med

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L-type calcium channels (LTCCs), the largest subfamily of voltage-gated calcium channels (VGCCs), are the main channels for Ca2+ influx during extracellular excitation. LTCCs are widely present in excitable cells, especially cardiac and cardiovascular smooth muscle cells, and participate in various Ca2+-dependent processes. LTCCs have been considered as worthy drug target for cardiovascular, neurological and psychological diseases for decades. Natural products from Traditional Chinese medicine (TCM) have shown the potential as new drugs for the treatment of LTCCs related diseases. In this review, the basic structure, function of LTCCs, and the related human diseases caused by structural or functional abnormalities of LTCCs, and the natural LTCCs antagonist and their potential usages were summarized.

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Section: Discussionmentioning

confidence: 99%

Natural L-type calcium channels antagonists from Chinese medicine

Xu,

Cai,

Liu

et al. 2024

Chin Med

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“…Therefore, to assess the function of the engineered RPE layer, we next incubated the cells with fluorescent latex beads (d = 1 µm), and the ability of the engineered RPE layer to perform phagocytosis was demonstrated (Figure 4h). Moreover, several key RPE functions, such as the transport of nutrients and waste products across it, the maintenance of the blood-retinal barrier, and the regulation of cell growth and differentiation, are regulated by calcium ions [34]. Therefore, we next sought to assess calcium signaling.…”

Section: Engineering the Rpe Layer On Top Of The Printed Vascular Layermentioning

confidence: 99%

Sequential Fabrication of a Three-Layer Retina-like Structure

Shechter,

Cohen,

Namestnikov

et al. 2024

Gels

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Tissue engineering is considered a promising approach to treating advanced degenerative maculopathies such as nonexudative age-related macular degeneration (AMD), the leading cause of blindness worldwide. The retina consists of several hierarchical tissue layers, each of which is supported by a layer underneath. Each of these layers has a different morphology and requires distinct conditions for proper assembly. In fact, a prerequisite step for the assembly of each of these layers is the organization of the layer underneath. Advanced retinal degeneration includes degeneration of the other retina layers, including the choroid, the retinal pigmented epithelium (RPE), and the photoreceptors. Here, we report a step-by-step fabrication process of a three-layer retina-like structure. The process included the 3D printing of a choroid-like structure in an extracellular matrix (ECM) hydrogel, followed by deposition of the RPE monolayer. After the formation of the blood vessel–RPE interface, the photoreceptor cells were deposited to interact with the RPE layer. At the end of the fabrication process, each layer was characterized for its morphology and expression of specific markers, and the integration of the three-layer retina was evaluated. We envision that such a retina-like structure may be able to attenuate the deterioration of a degenerated retina and improve engraftment and regeneration. This retinal implant may potentially be suitable for a spectrum of macular degenerative diseases for which there are currently no cures and may save millions from complete blindness.

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