Crowd Controlled—Host Quorum Sensing Drives Phage Decision

Harms, Alexander; Diard, Médéric

doi:10.1016/j.chom.2019.01.016

Cited by 12 publications

(13 citation statements)

References 10 publications

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“…An example of how enzyme can be applied conventionally is the use of enzyme detergents which have the advantages of being sensitive to materials that can be harmed by harsh chemicals or high temperatures. By using enzyme detergents that contain a mixture of protease, D-Nase I, amylase and cellulose, it can destabilize the biofilm EPS and improve the efficiency of biofilm removal [22,36].…”

Section: Eps Degradation Enzymesmentioning

confidence: 99%

“…Quorum sensing (QS) is a cell-to-cell communication system that controls gene expression in a cell density dependent manner [19,35]. QS regulates a diverse range of phenotypic expressions including virulence, motility, luminescence and biofilm formation [36]. QS signalling molecules, used to mediate bacterial ability to sense the surrounding cell density, have these particular characteristics: (i) production happens in specific growth stages or a feedback to certain environmental challenges; (ii) accumulate in the surrounding environment and be recognized by bacterial receptors; (iii) an accumulation of a threshold QS signal concentration results in physiological response; and (iv) results in phenotypic changes.…”

Section: Quorum Quenchingmentioning

confidence: 99%

“…In addition, the usage of the right enzyme such as benzonase could potentially have remarkable outcome due to its wide range of specificitydegrading all forms of DNA and RNA and its ability able to work over a broad range of pH and temperatures. As enzymes can have a short life span, immobilization could also be advantageous as it causes most enzymes to become more efficient and stable [17,36]. Kim JH showed that by immobilizing acylase on a surface, it was able to prohibit biofilm formation and maintain more than 90% enzymatic activity over an extended 20 reaction and washing cycles in nano-filtration [37].…”

Section: Quorum Quenchingmentioning

confidence: 99%

See 2 more Smart Citations

Biological and Physiochemical Methods of Biofilm Adhesion Resistance Control of Medical-Context Surface

Li¹,

Li²,

Yu³

et al. 2021

Int. J. Biol. Sci.

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Section: Eps Degradation Enzymesmentioning

confidence: 99%

Section: Quorum Quenchingmentioning

confidence: 99%

Section: Quorum Quenchingmentioning

confidence: 99%

See 1 more Smart Citation

Biological and Physiochemical Methods of Biofilm Adhesion Resistance Control of Medical-Context Surface

Li¹,

Li²,

Yu³

et al. 2021

Int. J. Biol. Sci.

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“…Contrasting the other four mechanisms of phage-associated communication (Figure 1), autoinducer-associated prophage induction (AAPI) [3,130,131] represents an outlier. Perhaps, however, this is to be expected given that it represents a form of bacteria-to-virus rather than virus-to-virus intercellular communication.…”

Section: Other Virus-associated Communication Mechanismsmentioning

confidence: 99%

Look Who’s Talking: T-Even Phage Lysis Inhibition, the Granddaddy of Virus-Virus Intercellular Communication Research

Abedon

2019

Viruses

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That communication can occur between virus-infected cells has been appreciated for nearly as long as has virus molecular biology. The original virus communication process specifically was that seen with T-even bacteriophages—phages T2, T4, and T6—resulting in what was labeled as a lysis inhibition. Another proposed virus communication phenomenon, also seen with T-even phages, can be described as a phage-adsorption-induced synchronized lysis-inhibition collapse. Both are mediated by virions that were released from earlier-lysing, phage-infected bacteria. Each may represent ecological responses, in terms of phage lysis timing, to high local densities of phage-infected bacteria, but for lysis inhibition also to locally reduced densities of phage-uninfected bacteria. With lysis inhibition, the outcome is a temporary avoidance of lysis, i.e., a lysis delay, resulting in increased numbers of virions (greater burst size). Synchronized lysis-inhibition collapse, by contrast, is an accelerated lysis which is imposed upon phage-infected bacteria by virions that have been lytically released from other phage-infected bacteria. Here I consider some history of lysis inhibition, its laboratory manifestation, its molecular basis, how it may benefit expressing phages, and its potential ecological role. I discuss as well other, more recently recognized examples of virus-virus intercellular communication.

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“…The experiment detected a hexapeptide moiety that is secreted during the lytic cycle, but encourages the phage to commit to the lysogenic mode as it accumulates. Release of the peptide communicates that phages have successfully lysed the bacterial host and increased [55] concentrations indicate more and more successful phages, and corresponding lower host abundance (high MOI), which encourages phages to switch to the lysogenic cycle [56]. In the absence of this signaling moiety, however, the phages are induced to switch into the lytic mode.…”

Section: Phage Communication Shapes Lytic-lysogenic Decisionmentioning

confidence: 99%

How to Train Your Phage: The Recent Efforts in Phage Training

et al. 2021

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Control of pathogenic bacteria by deliberate application of predatory phages has potential as a powerful therapy against antibiotic-resistant bacteria. The key advantages of phage biocontrol over antibacterial chemotherapy are: (1) an ability to self-propagate inside host bacteria, (2) targeted predation of specific species or strains of bacteria, (3) adaptive molecular machinery to overcome resistance in target bacteria. However, realizing the potential of phage biocontrol is dependent on harnessing or adapting these responses, as many phage species switch between lytic infection cycles (resulting in lysis) and lysogenic infection cycles (resulting in genomic integration) that increase the likelihood of survival of the phage in response to external stress or host depletion. Similarly, host range will need to be optimized to make phage therapy medically viable whilst avoiding the potential for deleteriously disturbing the commensal microbiota. Phage training is a new approach to produce efficient phages by capitalizing on the evolved response of wild-type phages to bacterial resistance. Here we will review recent studies reporting successful trials of training different strains of phages to switch into lytic replication mode, overcome bacterial resistance, and increase their host range. This review will also highlight the current knowledge of phage training and future implications in phage applications and phage therapy and summarize the recent pipeline of the magistral preparation to produce a customized phage for clinical trials and medical applications.

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Crowd Controlled—Host Quorum Sensing Drives Phage Decision

Cited by 12 publications

References 10 publications

Biological and Physiochemical Methods of Biofilm Adhesion Resistance Control of Medical-Context Surface

Biological and Physiochemical Methods of Biofilm Adhesion Resistance Control of Medical-Context Surface

Look Who’s Talking: T-Even Phage Lysis Inhibition, the Granddaddy of Virus-Virus Intercellular Communication Research

How to Train Your Phage: The Recent Efforts in Phage Training

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