Crowding and confinement effects on enzyme stability in mesoporous silicas

Shin, Soo Jai; Kim, Han Sol; Kim, Moon I. I.; Lee, Jun Young; Park, Hyun Gyu; Kim, Jungbae

doi:10.1016/j.ijbiomac.2019.12.034

Cited by 18 publications

(16 citation statements)

References 48 publications

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“…Mesoporous SiO 2 has a typical honeycomb channel structure and significant loading and retention capabilities. The size of SiO 2 nanoparticles is generally between 250–400 nm [ 7 ]. It is a very good drug delivery system, especially as a nanocarrier for drugs, gene delivery, and bioimaging and photodynamic therapy [ 8 , 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Chitosan Derivatives Modified Mesoporous Silica Nanoparticles as Delivery Carrier

Wang

et al. 2021

Molecules

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Chitosan is a non-toxic biological material, but chitosan is insoluble in water, which hinders the development and utilization of chitosan. Chitosan derivatives N-2-Hydroxypropyl trimethyl ammonium chloride (N-2-HACC) and carboxymethyl chitosan (CMCS) with good water solubility were synthesized by our laboratory. In this study, we synthesized mesoporous SiO2 nanoparticles by the emulsion, and then the mesoporous SiO2 nanoparticles were modified with γ-aminopropyltriethoxysilane to synthesize aminated mesoporous SiO2 nanoparticles; CMCS and N-2-HACC was used to cross-link the aminated mesoporous SiO2 nanoparticles to construct SiO2@CMCS-N-2-HACC nanoparticles. Because the aminated mesoporous SiO2 nanoparticles with positively charged can react with the mucous membranes, the virus enters the body mainly through mucous membranes, so Newcastle disease virus (NDV) was selected as the model drug to evaluate the performance of the SiO2@CMCS-N-2-HACC nanoparticles. We prepared the SiO2@CMCS-N-2-HACC nanoparticles loaded with inactivated NDV (NDV/SiO2@CMCS-N-2-HACC). The SiO2@CMCS-N-2-HACC nanoparticles as delivery carrier had high loading capacity, low cytotoxicity, good acid resistance and bile resistance and enteric solubility, and the structure of NDV protein encapsulated in the nano vaccine was not destroyed. In addition, the SiO2@CMCS-N-2-HACC nanoparticles could sustain slowly released NDV. Therefore, the SiO2@CMCS-N-2-HACC nanoparticles have the potential to be served as delivery vehicle for vaccine and/or drug.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Chitosan Derivatives Modified Mesoporous Silica Nanoparticles as Delivery Carrier

Wang

et al. 2021

Molecules

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“…Inside cells, proteins exist and function in highly crowded and compartmentalized environments that have a significant impact on several of their functions, including diffusion, enzymatic activity, protein-protein interactions, and folding, unfolding, and refolding [5,6]. Several studies have proposed the use of high concentrations of natural and synthetic macromolecules to study crowding [7][8][9][10], and encapsulating proteins within the pores of silica, polyacrylamide, or other hydrogels to study confinement [11][12][13][14][15][16][17]. These studies have contributed to the mechanistic understanding of how proteins behave in vivo, as well as to unravel the differences between how confinement and crowding influence protein function [14,16,17].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have proposed the use of high concentrations of natural and synthetic macromolecules to study crowding [7][8][9][10], and encapsulating proteins within the pores of silica, polyacrylamide, or other hydrogels to study confinement [11][12][13][14][15][16][17]. These studies have contributed to the mechanistic understanding of how proteins behave in vivo, as well as to unravel the differences between how confinement and crowding influence protein function [14,16,17]. The latter outcome is significant because confinement and crowding are often used synonymously, even though these environments are distinct in how they exert their influence on protein fates.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…In this study, we describe our attempts to build on previous research and develop conditions to study the combined effects of confinement and crowding on protein stability [17,[24][25][26]. These studies are relevant as recent studies have shown that investigations of protein fates in the combined presence of confinement and crowding can shed light on the underlying mechanisms of both single-and multi-domain protein folding and stability in vivo [17,26]. We investigated the combined effects of confinement and crowding on the functional and structural stability of two model proteins, horseradish peroxidase (HRP) and β-galactosidase (β-gal), using agarose scaffolds incorporating various amounts of dextran, an inert polysaccharide.…”

Section: Introductionmentioning

confidence: 99%

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Combined Effects of Confinement and Macromolecular Crowding on Protein Stability

Ross

Kunkel

Long

et al. 2020

IJMS

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Confinement and crowding have been shown to affect protein fates, including folding, functional stability, and their interactions with self and other proteins. Using both theoretical and experimental studies, researchers have established the independent effects of confinement or crowding, but only a few studies have explored their effects in combination; therefore, their combined impact on protein fates is still relatively unknown. Here, we investigated the combined effects of confinement and crowding on protein stability using the pores of agarose hydrogels as a confining agent and the biopolymer, dextran, as a crowding agent. The addition of dextran further stabilized the enzymes encapsulated in agarose; moreover, the observed increases in enhancements (due to the addition of dextran) exceeded the sum of the individual enhancements due to confinement and crowding. These results suggest that even though confinement and crowding may behave differently in how they influence protein fates, these conditions may be combined to provide synergistic benefits for protein stabilization. In summary, our study demonstrated the successful use of polymer-based platforms to advance our understanding of how in vivo like environments impact protein function and structure.

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Rational Development of Co‐Doped Mesoporous Ceria with High Peroxidase‐Mimicking Activity at Neutral pH for Paper‐Based Colorimetric Detection of Multiple Biomarkers

Nguyen

Lee

Cho

et al. 2022

Adv Funct Materials

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Peroxidase-mimicking nanozymes have been extensively studied, however, their application is limited by the requirement for an acidic pH. Herein, the development of Co-doped mesoporous cerium oxide (Co-m-ceria) is reported, which operates optimally at a near-neutral pH and exhibits a peroxidaselike catalytic efficiency that is 600-times higher than that of pristine m-ceria. Density functional theory (DFT) calculations for the application of pristine and various metal-doped m-ceria in peroxidase-like reactions under different pH environments are conducted to select Co as the appropriate dopant. The high peroxidase-like activity of Co-m-ceria under neutral conditions and its mesoporous nature enable its application in a one-pot cascade reaction system, wherein biomarkers of oxidative enzymes can be detected without altering the pH. Five different oxidative enzymes are immobilized in the pores of Co-m-ceria at high loadings, followed by incorporation of the enzymecontaining Co-m-ceria in paper microfluidic devices for the convenient and simultaneous detection of multiple biomarkers. The Co-m-ceria-incorporated paper microfluidic device enables the selective and sensitive determination of multiple biomarkers using a smartphone-acquired image. This study demonstrates the potential of the rational design of nanozymes and their application in paper microfluidic devices, laying the groundwork for future applications of nanozymes in point-of-care testing environments.

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Crowding and confinement effects on enzyme stability in mesoporous silicas

Cited by 18 publications

References 48 publications

Evaluation of Chitosan Derivatives Modified Mesoporous Silica Nanoparticles as Delivery Carrier

Evaluation of Chitosan Derivatives Modified Mesoporous Silica Nanoparticles as Delivery Carrier

Combined Effects of Confinement and Macromolecular Crowding on Protein Stability

Rational Development of Co‐Doped Mesoporous Ceria with High Peroxidase‐Mimicking Activity at Neutral pH for Paper‐Based Colorimetric Detection of Multiple Biomarkers

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