Gathering physiological data when they are performing experiments requires a great effort from researchers. Very often, a considerable time is required to prepare the signal acquisition equipment, conduct the experiments, and properly label the data of each participant. Nevertheless this data is valuable for the analysis of personal characteristics, such as behavior, health conditions, and preferences. With the aim of assisting researchers with such tedious tasks, we have developed the DAFIESKU system. This system serves to acquire several types of physiological data. DAFIESKU facilitates the creation of new datasets with physiological data by means of mobile and wearable devices. The usability of the system was evaluated in two case studies in a two-step iterative process. Before conducting the second case study, the whole system was improved using the feedback obtained from the first case study. The results achieved show that usability was enhanced in the second version of DAFIESKU.