Crown gall incidence in plant nurseries of Algeria, characteristics of Agrobacterium tumefaciens strains, and biological control of strains sensitive and resistant to agrocin 84

Bouzar, H.; Daouzli, N.; Krimi, Zoulikha; Alim, Achmad Saiful; Khemici, E.

doi:10.1051/agro:19911007

Cited by 16 publications

(11 citation statements)

References 18 publications

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“…They are usually responsible for gall disease in vegetables. This bacterium was also found by Bouzar et al [22], who showed that the fruits in tree nurseries of Algeria were infected by this germ and the most affected botanical species were peach tree, almond tree, cherry tree, apple tree and olive tree.…”

Section: Analysis Of Pcr-dgge Profilessupporting

confidence: 73%

Application of PCR-DGGE to the study of dynamics and biodiversity of microbial contaminants during the processing ofHibiscus sabdariffadrinks and concentrates

Ndiaye

Hamdouche²,

Kane

et al. 2016

Fruits

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-Introduction. Bissap (Hibiscus sabdariffa L.) is a common plant in the tropics. In Senegal, the calyces are used to make a popular juice. In the food industry, small and medium-sized enterprises (SMEs) are responsible for the transformation of bissap calyces into drinks, concentrates, jam, etc. In spite of the very low pH of the juice (pH < 3), problems of contamination and fermentation are often observed in the final products post-pasteurization. They are mainly due to Pseudomonas spp., E. coli, Klebsiella spp. and Pichia opuntiae. To solve this issue, monitoring of the microbial ecology was performed during the full process of bissap products. Methods and results. Fresh calyces and dried mixed calyces of the two varieties of Hibiscus sabdariffa ('Koor' and 'Vimto'), as well as juice samples, were collected at every stage of the processing of a bissap drink and syrup in a Senegalese SME. The monitoring of microbial flora was performed by using molecular fingerprinting. The molecular technique PCR-DGGE was employed to evaluate the microbial dynamics using bacterial 16S rDNA, yeast 26S rDNA and 28S rDNA mold profiles at each critical stage of the process. Results and discussion. The genetic profiles generated contributed to identifying the critical points in the manufacturing processes. A multivariate analysis based on the presence or absence of spots in the denaturing gradient electrophoresis gels (DGGE) showed that the microbial flora (bacteria, yeasts, molds) of bissap evolved during the following phases: harvest (fresh flower), drying (dried calyces) and processing (before filtration, after pasteurization and before packaging). Conclusion. Our work contributed to determining the microorganisms responsible for the microbial contamination of the final products, and highlighted the origin of these contaminants. The most important critical point was identified as the pasteurization step.

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Section: Analysis Of Pcr-dgge Profilessupporting

confidence: 73%

Application of PCR-DGGE to the study of dynamics and biodiversity of microbial contaminants during the processing ofHibiscus sabdariffadrinks and concentrates

Ndiaye

Hamdouche²,

Kane

et al. 2016

Fruits

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“…Selective isolation of bv. 1 and/or pseudomonads by this medium has been reported elsewhere (5,10). It should be noted, however, that RS and MG media were useful in complementing the other two media for the isolation of additional strains, some of which had peculiar chromosome and plasmid phenotypes.…”

Section: Discussionmentioning

confidence: 65%

“…This paucity of information is due in part to the rare detection of tumorigenic strains in the soil and on roots, even though nontumorigenic agrobacteria are commonly found (2,7,10,35,52). These apparently low levels of tumorigenic bacteria in soil are in sharp contrast to the regular occurrence of crown gall epidemics and the efficacious protection against soil infections by the biocontrol agent K84 (5,35).…”

mentioning

confidence: 87%

Correlative Association between Resident Plasmids and the Host Chromosome in a Diverse Agrobacterium Soil Population

Bouzar

Ouadah

Krimi

et al. 1993

Appl Environ Microbiol

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Soil samples collected from a fallow field which had not been cultivated for 5 years harbored a population ofAgrobacterium spp. estimated at 3 x 107 CFU/g. Characterization of 72 strains selected from four different isolation media showed the presence of biovar 1 (56%) and bv. 2 (44%) strains. Pathogenicity assays on five different test plants revealed a high proportion (33%) of tumorigenic strains in the resident population. All tumorigenic strains belonged to bv. 1. Differentiation of the strains by restriction fragment length polymorphism analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of cellular proteins, and utilization patterns of 95 carbon substrates (Biolog GN microplate) revealed a diversified bv. 1 population, composed of five distinct chromosomal backgrounds (chr A, C, D, E, and F), and a homogeneous bv. 2 population (chr B). chr A, B, C, and D were detected at similar levels throughout the study site. According to opine metabolism, pathogenicity, and agrocin sensitivity, chr A strains carried a nopaline Ti plasmid (pTi), whereas chr C strains had an octopine pTi. In addition, four of six nontumorigenic bv. 1 strains (two chr D, one chr E, and one chr F) had distinct and unusual opine catabolism patterns. chr B (bv. 2) strains were nonpathogenic and catabolized nopaline. Although agrocin sensitivity is a pTi-borne trait, 14 chr B strains were sensitive to agrocin 84, apparently harboring a defective nopaline pTi similar to pAtK84b. The other two chr B strains were agrocin resistant. The present analysis of chromosomal and plasmid phenotypes suggests that in this Agrobacterium soil population, there is a preferential association between the resident plasmids and their bacterial host.

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“…Ainsi, le pourcentage de souches sensibles à K 84 est plus élevé chez le biovar 2 (92%) que le biovar 1 (71%), alors qu'il reste élevé (92 -97%) pour la K 1026 pour les deux biovars. Ces résultats sont différents de ceux obtenus par Schroth & Moller (1976), López et al (1989) et Bouzzar et al (1991 avec la K 84 et nous estimons que l'utilisation du milieu MG dans notre travail a augmenté la qualité de l'antagonisme in vitro par rapport au milieu de Stonier (1960) utilisé par ces auteurs. La K 1026 est d'utilisation récente, datant des années 1998.…”

Section: Influence Du Biovarunclassified

Premiers résultats de lutte biologique par les souches K₈₄ et K₁₀₂₆ contre Agrobacterium radiobacter var. tumefaciens, agent du crown‐gall des rosacées fruitières et du rosier au Maroc

Benjama

Redwane²,

Gadda

et al. 2002

EPPO Bulletin

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Une collection de 206 souches marocaines d’Agrobacterium radiobacter var. tumefaciens a été testée vis‐à‐vis des souches antagonistes Agrobacterium radiobacter K84 et K1026in vitro. Ce test d’antagonisme a montré que la sensibilité des souches varie en fonction de la souche antagoniste, du biovar et de la plante‐hôte d’origine. La K1026 s’est avérée meilleure que la K84 aussi bien par le nombre et par le diamètre plus élevé. Des essais au champ ont montré une bonne efficacité de la souche antagoniste K84 en seaux ou en sol naturellement infesté. La réussite de la lutte biologique avec la K84 varie entre 70% et 95% sur GF677, rosier, amandier amer et pêcher par rapport aux témoins sans K84, alors que l’abricotier s’est montré résistant au crown‐gall aussi bien en sol inoculé qu’en sol naturellement infesté. Nous pensons généraliser cette lutte dans tous les foyers marocains à crown‐gall avec une souche mutante de la K84, la K1026, qui a donné aussi une satisfaction meilleure in vitro dans d’autres pays méditerranéens comme l’Espagne, la France et l’Italie.

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Crown gall incidence in plant nurseries of Algeria, characteristics of Agrobacterium tumefaciens strains, and biological control of strains sensitive and resistant to agrocin 84

Abstract: Summary ― Crown gall was observed in 99% of the plant nurseries surveyed in the major fruit tree production region of Algeria. Among 11 tree species surveyed, those with highest disease incidence were peach (5.39%), almond (3.43%), cherry (1.50%), apple (1.47%), and olive (1.30%). None

Cited by 16 publications

References 18 publications

Application of PCR-DGGE to the study of dynamics and biodiversity of microbial contaminants during the processing ofHibiscus sabdariffadrinks and concentrates

Application of PCR-DGGE to the study of dynamics and biodiversity of microbial contaminants during the processing ofHibiscus sabdariffadrinks and concentrates

Correlative Association between Resident Plasmids and the Host Chromosome in a Diverse Agrobacterium Soil Population

Premiers résultats de lutte biologique par les souches K₈₄ et K₁₀₂₆ contre Agrobacterium radiobacter var. tumefaciens, agent du crown‐gall des rosacées fruitières et du rosier au Maroc

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Crown gall incidence in plant nurseries of Algeria, characteristics of Agrobacterium tumefaciens strains, and biological control of strains sensitive and resistant to agrocin 84

Abstract: Summary ― Crown gall was observed in 99% of the plant nurseries surveyed in the major fruit tree production region of Algeria. Among 11 tree species surveyed, those with highest disease incidence were peach (5.39%), almond (3.43%), cherry (1.50%), apple (1.47%), and olive (1.30%). None

Cited by 16 publications

References 18 publications

Application of PCR-DGGE to the study of dynamics and biodiversity of microbial contaminants during the processing ofHibiscus sabdariffadrinks and concentrates

Application of PCR-DGGE to the study of dynamics and biodiversity of microbial contaminants during the processing ofHibiscus sabdariffadrinks and concentrates

Correlative Association between Resident Plasmids and the Host Chromosome in a Diverse Agrobacterium Soil Population

Premiers résultats de lutte biologique par les souches K84 et K1026 contre Agrobacterium radiobacter var. tumefaciens, agent du crown‐gall des rosacées fruitières et du rosier au Maroc

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Premiers résultats de lutte biologique par les souches K₈₄ et K₁₀₂₆ contre Agrobacterium radiobacter var. tumefaciens, agent du crown‐gall des rosacées fruitières et du rosier au Maroc