CRP/CD11b ratio: a novel parameter for detecting gram-positive sepsis

Kinetics of leukocyte CD11b and CD64 expression in severe sepsis and non‐infectious critical care patients

“…In most earlier studies, the results have been presented unstandardized, as percents of antigen‐positive cells or as mean fluorescence intensity values . In contrast to our study, earlier publications of CD11b and CD64 expression have mainly been focused on non‐ICU populations (e.g., emergency room patients or hospital wards) . Furthermore, in studies concerning ICU‐treated severe sepsis or septic shock, controls have not typically represented ICU populations …”

“…In our study, the samples were taken on 3 consecutive days. Some studies have reported on the expression kinetics of leukocyte surface molecules in infection, but most reports are based on one time point . However, the disease process and the time when treatment starts may greatly vary in sepsis, and conclusions based on laboratory tests should be cautiously made by a single time point .…”

“…In resting cells, CD11b is stored intracellularly and rapidly recruited to the cell membrane upon activation of the cells in inflammation . Reports on the ability of CD11b expression to distinguish sepsis from non‐septic inflammation are scarce and conflicting . CD64 is a high‐affinity Fcγ receptor I expressed constitutively on monocytes and on neutrophils upon activation .…”

Monocytic and neutrophilic CD11b and CD64 in severe sepsis

“…Leukocyte immunophenotyping could improve sepsis diagnostics [1,2]. Our hypothesis was that monocytic and neutrophilic CD11b and CD64 antigen fluorescence intensities differ between severe sepsis, non-inflammatory ICU patients and nonseptic inflammation (off-pump coronary artery bypass (OPCABG)).…”

Leukocyte immunophenotyping: methodological aspects

“…Flow cytometric analysis of leukocyte surface receptors has been performed and shown beneficial, for example to characterize infectious and septic patients [1,2]. For many surface antigens the results may vary depending on the sampling temperature, the anticoagulant used and the storage of the sample before analysis [3].…”