CRP determination based on a novel magnetic biosensor

Meyer, M Cobas; Hartmann, Markus; Krause, Hans‐Joachim; Blankenstein, Gert; Mueller-Chorus, Birgit; Oster, Juergen; Miethe, Peter; Keusgen, Michael

doi:10.1016/j.bios.2006.04.001

Cited by 132 publications

(84 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…alternating and rotating) has recently become a potent tool for detection of proteins and molecules bound to the NPs. [1][2][3][4][5][6] To conduct a successful homogeneous magnetic bioassay, particularly in a rotating magnetic field (RMF), the iron oxide NP aqueous suspensions relaxing via the Brownian mechanism (d core > 24 nm) are required.…”

Section: Introductionmentioning

confidence: 99%

Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays

et al. 2013

View full text Add to dashboard Cite

CitationHighly Uniformly sized and shaped iron oxide nanoparticles with a mean size of 25 nm were synthesized via decomposition of ironoleate. High resolution transmission electron microscopy and Mössbauer spectroscopy investigations revealed that the particles are spheres primarily composed of Fe 3 O 4 with a small fraction of FeO. From Mössbauer and static magnetization measurements, it was deduced that the particles are superparamagnetic at room temperature. The hydrophobic particles were successfully transferred into water via PEGylation using nitrodopamine as an anchoring group. IR spectroscopy and thermogravimetric analysis showed the success and efficiency of the phase transfer reaction. After the PEGylation, the particles retained monodisperse and their magnetic core remained intact as proven by photon cross-correlation spectrocopy, ac susceptibility, and transmission electron microscopy. The particle aqueous suspensions revealed an excellent water stability over a month of monitoring and also against temperature up to 40 • C. The particles exhibited a moderate cytotoxic effect on in vitro cultured bone marrow-derived macrophages and no release of inflammatory or anti-inflammatory cytokines. The PEGylated particles were functionalized with Herceptin antibodies via a conjugation chemistry, their response to a rotating magnetic field was studied using a fluxgate-based setup and was compared with the one recorded for hydrophobic and PEGylated particles. The particle phase lag rose after labeling with Herceptin, indicating the successful conjugation of Herceptin antibodies to the particles.

show abstract

Section: Introductionmentioning

confidence: 99%

Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays

et al. 2013

View full text Add to dashboard Cite

show abstract

“…AuNPs on the surface have increased the sensitivity of the sensor. This method is more sensitive compared with other measurement methods based on related data have shown that (Meyer et al, 2002, Meyer et al, 2007Vikholm-Lundin et al, 2006). 87fM be determined until this study, low-range signal.…”

Section: Qcm Based Biosensors Developed For Determination Of Crpmentioning

confidence: 80%

Biosensors for Cancer Biomarkers

Uygun¹,

Sezgintürk²

2011

Biosensors - Emerging Materials and Applications

View full text Add to dashboard Cite

“…The H-terminated NCD samples (homemade and Rho-BeSt) were immersed in this anti-CRP solution and incubated for 2 hours at 37 °C to allow physisorption of the antibodies through hydrophobic interactions, which results in reliable attachments [15,27]. The anti-CRP-coated NCD samples were subsequently incubated overnight in a 6% BSA solution in 1× PBS at 4 °C to block the remainder of the NCD surface that was not coated with anti-CRP.…”

Section: Ncd Synthesis and Functionalisationmentioning

confidence: 99%

“…Recently, these same authors developed a novel CRP determination method based on magnetic detection. They showed to be more sensitive than the typical hs-CRP ELISA assays [15]. In the last years, the focus on EIS-based immunosensing methods has increased significantly.…”

mentioning

confidence: 99%

Synthetic diamond films as a platform material for label‐free protein sensors

Bijnens

Vermeeren

Daenen

et al. 2009

Physica Status Solidi (a)

View full text Add to dashboard Cite

In the framework of developing a fast and label‐free immunosensor for C‐reactive protein (CRP) detection, H‐terminated nanocrystalline diamond (NCD) was functionalised with anti‐CRP antibodies that were physically adsorbed to the surface. Impedance spectroscopy was used to electronically detect real‐time CRP recognition. Different impedance behaviours were observed after CRP addition as compared to after FITC‐labelled ssDNA addition at low (100 Hz) as well as at high frequencies (1 MHz). Physical interpretations of the observed impedance changes were obtained by fitting the data to an equivalent electrical circuit. Concentrations of 1 μM CRP were recognised with a reaction time of 30 minutes. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

show abstract

CRP determination based on a novel magnetic biosensor

Cited by 132 publications

References 32 publications

Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays

Highly stable monodisperse PEGylated iron oxide nanoparticle aqueous suspensions: a nontoxic tracer for homogeneous magnetic bioassays

Biosensors for Cancer Biomarkers

Synthetic diamond films as a platform material for label‐free protein sensors

Contact Info

Product

Resources

About