Crucial factors governing the electrochemical impedance on protein-modified surfaces

Sopoušek, Jakub; Věžník, Jakub; Houser, Josef; Skládal, Petr; Lacina, Karel

doi:10.1016/j.electacta.2021.138616

Cited by 13 publications

(6 citation statements)

References 27 publications

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“…The observed DEIS effects revealed the complexity of the studied biosystems where the electrostatic and charging processes play an important role in the protein-protein interactions [27] , [76] . Other plausible charge effects affecting the migration of the redox ions by altering the functionalisation layer geometry at the polarised electrode surface are visualised in Fig.…”

Section: Resultsmentioning

confidence: 97%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Brodowski¹,

Pierpaoli²,

Janik³

et al. 2022

Sensors and Actuators B: Chemical

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Section: Resultsmentioning

confidence: 97%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Brodowski¹,

Pierpaoli²,

Janik³

et al. 2022

Sensors and Actuators B: Chemical

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“…The observed DEIS effects revealed the complexity of the studied biosystems where the electrostatic and charging processes play an important role in the protein-protein interactions (Niedzialkowski et al, 2020;Sopoušek et al, 2021). Other plausible charge effects affecting the migration of the redox ions by altering the functionalisation layer geometry at the polarised electrode surface are visualised in Fig.…”

Section: Dmentioning

confidence: 92%

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: multivariate data analysis of multisine impedimetric response

Brodowski

Pierpaoli

Janik

et al. 2022

Preprint

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Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) enters the cells through the binding of spike protein to the host cell surface-expressing angiotensin-converting enzyme 2 (ACE2) or by endocytosis mediated by extracellular matrix metalloproteinase inducer (CD147). We present extended statistical studies of the multisine dynamic electrochemical impedance spectroscopy (DEIS) revealing interactions between Spike RBD and cellular receptors ACE2 and CD147, and a reference anti-RBD antibody (IgG2B) based on a functionalised boron-doped diamond (BDD) electrode. The DEIS was supported by a multivariate data analysis of a SARS-CoV-2 Spike RBD assay and cross-correlated with the atomic-level information revealed by molecular dynamics simulations. Our approach allowed us to study and detect subtle changes in the electrical properties responsible for the susceptibility of cellular receptors to SARS-CoV-2, revealing their interactions. Changes in electrical homogeneity in the function of the RBD concentration led to the conclusion that the ACE2 receptor delivers the most homogeneous surface, delivered by the high electrostatic potential of the relevant docking regions. For higher RBD concentrations, the differences in electrical homogeneity between electrodes with different receptors vanish. Collectively, this study reveals interdependent virus entry paths involving separately ACE2, CD147, and spike protein, provided by a developed biosensing platform for the rapid screening of cellular interactions (i.e. testing various mutations of SARS-CoV-2 or screening of therapeutic drugs).

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“…Commonly, impedance applications are related to affinity sensors designs. 198 A novel EIS biosensor was developed by Bahner et al for the detection of doxorubicin, a pervasive cancer treatment drug. 199 The sensor is based on the daunorubicin-binding aptamer, used as recognition element, and is immobilized with mercaptohexanol on gold.…”

Section: Electrochemical Nanosensorsmentioning

confidence: 99%

Review—Bio-Nanosensors: Fundamentals and Recent Applications

Perdomo

Marmolejo‐Tejada

Jaramillo-Botero

2021

J. Electrochem. Soc.

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Crucial factors governing the electrochemical impedance on protein-modified surfaces

Cited by 13 publications

References 27 publications

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: Multivariate data analysis of multisine impedimetric response

Enhanced susceptibility of SARS-CoV-2 spike RBD protein assay targeted by cellular receptors ACE2 and CD147: multivariate data analysis of multisine impedimetric response

Review—Bio-Nanosensors: Fundamentals and Recent Applications

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