Crude enzymatic extract from Dictyopanus pusillus LMB4 as a biotechnological tool for crystal violet biodegradation

Rueda-Villabona, Jesús D.; Rueda, Andrés M.; Saavedra, Olga L.; Hernández, Inés; Rincón, Giovanna; Martínez-Vega, Ruth Aralí; Sánchez, Clara Inés

doi:10.1016/j.biteb.2021.100723

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…In the past, several physico-chemical technologies have been studied for dye degradation in wastewater, including filtration, adsorption, photodegradation, and irradiation (Parshetti et al 2006 ). Conventional physico-chemical treatments are effective, but ineffective when the dye is present in low concentrations, whereas sometimes it also generates toxic compounds (organochlorides and aromatic amines) that could be more toxic than the dye prior to pretreatment (Rueda-Villabona et al 2021 ). Pretreatment using bacteria or enzymes has the advantage of total dye decolorization and breakdown of its intermediates as well as decreasing negative environmental effects (Patel and Patel 2020 ).…”

Section: Introductionmentioning

confidence: 99%

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Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Albulaihed,

Adnan,

Jamal

et al. 2023

Bioresour. Bioprocess.

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Enzymatic degradation of synthetic dyes holds an immense promise for addressing the environmental concerns associated with the textile and dye industries. This study aimed to isolate bacteria capable of producing laccase enzymes from an anthropogenic environment. Subsequently, viability of utilizing cost-effective agricultural residues as substrates for laccase production was assessed. Response Surface Methodology (RSM) and the One Variable at a Time (OVAT) approach was pursued for the optimization of laccase production, followed by pH and temperature stability, dye degradation and decolorization experiments, toxicological studies on the degraded dye metabolites. In results, laccase-producing bacterial strain was identified as Stenotrophomonas maltophilia strain E1 (S. maltophilia). Among variety of substrates, coconut husk exhibited optimal efficacy. In a statistical optimization study, it was found that S. maltophilia was capable of producing laccase 51.38 IU/mL, i.e., three times higher than the amount of laccase produced by unoptimized medium (16.7 IU/mL), and the enzyme activity was found to be steady at an acidic pH, and a mesophilic temperature range. The laccase obtained from S. maltophilia E1 demonstrated proficient dye decolorization capabilities, achieving a notable 92.1% reduction in Malachite green dye coloration at a concentration of 500 ppm. Gas chromatography–mass spectrometry (GC–MS) analysis of the decolorized derivatives of Malachite green revealed a conversion into a distinct compounds. Moreover, after undergoing laccase treatment, Malachite green exhibited decreased phytotoxic effects on Oryza sativa, pointing to enzymatic detoxification. Collectively, insights gained from the present study will contribute to the development of efficient enzymatic approaches for addressing the environmental pollution caused by synthetic dyes. Graphical Abstract

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Section: Introductionmentioning

confidence: 99%

“…Biodegradation of dye is performed enzymatically through reduction, oxidation, hydroxylation and methylation reaction of dye’s structures (Rueda-Villabona et al 2021 ). Laccase from bacteria and fungi are reported for their dye’s bioremediation potential.…”

Section: Introductionmentioning

confidence: 99%

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Albulaihed,

Adnan,

Jamal

et al. 2023

Bioresour. Bioprocess.

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Laccase: A Catalyst for Change in Eco-Friendly Industrial Processes

Sampaio,

da Silva Quinto,

de Moura

et al. 2024

Engineering Materials

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Dynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green

Lima

Gomes-Pepe

Kock

et al. 2023

World J Microbiol Biotechnol

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LacMeta is suitable for the complete degradation and detoxi cation of malachite green (MG) dye and did not form leucomalachite (LCM, analogous compound). The optimal pH and temperature parameters of LacMeta were 5.0 and 50 °C, respectively (MG as substrate). LacMeta was tolerant of up to 10 mmol L-1 EDTA (82%ds) and up to 5% (V/V) acetone (91%ds) and methanol (71%ds), while SDS promoted severe inhibition. For ions, a high tolerance to cobalt, zinc, manganese, and calcium (10 mmol L-1) was also observed (> 90%ds). Even under high-salinity conditions (1 mol L-1 NaCl), the residual bleaching activity of the dye remained at 61%ds. Furthermore, the bleaching product of MG did not inhibit the germination of sorghum and tomato seeds and was inert to the vegetative structures of the germinated seedlings.Additionally, this treatment effectively reduced the cytotoxic effect of the dye on microorganisms (Escherichia coli and Azospirillum brasilense), which can be explained by H-NMR spectral analysis results since LacMeta completely degraded the peak signals corresponding to the aromatic rings in the dye, demonstrating extreme e ciency in the bioremediation of the xenobiotic at high concentrations (50 mg L-1).

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Crude enzymatic extract from Dictyopanus pusillus LMB4 as a biotechnological tool for crystal violet biodegradation

Cited by 3 publications

References 42 publications

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Optimization of laccase from Stenotrophomonas maltophilia E1 by submerge fermentation using coconut husk with its detoxification and biodecolorization ability of synthetic dyes

Laccase: A Catalyst for Change in Eco-Friendly Industrial Processes

Dynamics of the role of LacMeta laccase in the complete degradation and detoxification of malachite green

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