2019
DOI: 10.1101/768010
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Crumbs organizes the transport machinery by regulating apical levels of PI(4,5)P2inDrosophila

Abstract: 28An efficient vectorial intracellular transport machinery depends on a well-established apico-29 basal polarity and is a prerequisite for the function of secretory epithelia. Despite extensive 30 knowledge on individual trafficking pathways, little is known about the mechanisms 31 coordinating their temporal and spatial regulation. Here, we report that the polarity protein 32 Crumbs is essential for apical plasma membrane phospholipid-homeostasis and efficient 33 apical secretion. Through recruiting Heavy-Spe… Show more

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Cited by 2 publications
(2 citation statements)
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“…Notably, Moesin, which outlines PAACs in confocal micrographs (Fig. 3 and Video 3), is a marker of microvilli in other Drosophila epithelia (Edwards et al, 1997;Lattner et al, 2019). We also found sausage-shaped (allantoid) ultrastructures that, like the ellipsoids, are lined with dense microvilli and circumscribed by SJs (Fig.…”
Section: Paacs Are Intercellular Lumens Formed By Asymmetric Pre-enterocyte Apical Membranes and Enterocyte Basolateral Membranesmentioning
confidence: 52%
“…Notably, Moesin, which outlines PAACs in confocal micrographs (Fig. 3 and Video 3), is a marker of microvilli in other Drosophila epithelia (Edwards et al, 1997;Lattner et al, 2019). We also found sausage-shaped (allantoid) ultrastructures that, like the ellipsoids, are lined with dense microvilli and circumscribed by SJs (Fig.…”
Section: Paacs Are Intercellular Lumens Formed By Asymmetric Pre-enterocyte Apical Membranes and Enterocyte Basolateral Membranesmentioning
confidence: 52%
“…In vivo, Syx is bound to the CRUMBS complex via Mupp-1 (6,74,75). Given the membrane trafficking at this site, it would make sense that the PH domain of this protein would likely bind PI(4,5)P2, the canonical ligand for PH domains(9, 76), because PI(4,5)P2 is also associated with actin cytoskeletal activity modulated by the CRUMBS polarity complex (77,78). Active RhoA stimulates PIP 5-kinase and subsequent formation of PI(4,5)P2, implicating a positive feedback mechanism where active RhoA results in production of PI(4,5)P2, which recruits Syx to reactivate RhoA as part of a cell's apical cell polarity program (79,80).…”
Section: Discussionmentioning
confidence: 99%