Crushed bone grafts and a collagen membrane are not suitable for enhancing cartilage quality in the regeneration of osteochondral defects—An in vivo study in sheep

Schell, Hanna; Lienau, Jasmin; Kleemann, Ralf U.; Schlichting, Karin; Taylor, William R.; Weiler, Andreas; Duda, Georg N.

doi:10.1016/j.jbiomech.2007.02.021

Cited by 8 publications

(5 citation statements)

References 45 publications

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“…The same stain was used in the past to determine the chondrocyte clusters in the NP. 2,31 The present study showed that the Safranin O/FCF stain was best one for histopathological evaluations involving LDD, because it allows greater accuracy of the results, as it is easy to delimit the chondrocyte clusters in the NP.…”

Section: Discussionmentioning

confidence: 75%

Histological Markers of Degeneration and Regeneration of the Human Intervertebral Disk

et al. 2017

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Objective: To define histological scores for intervertebral disc degeneration that would enable the definition of morphological characteristics of disease, besides improving knowledge of the lumbar degenerative disc disease by means of immunohistochemical markers. Methods: Hematoxylin and Eosin, Alcian/PAS, Masson Trichrome and Safranin O/FCF staining was used on the intervertebral disc degeneration sections of patients with lumbar degenerative disc disease. The protein markers defined in immunohistochemistry were cell proliferation (Ki-67) and apoptosis (p53). Results: The study data enabled the determination of Safranin O/FCF stain as the most effective one for evaluating parameters such as area, diameter, and number of chondrocyte clusters. The importance of using stains in association, such as Safranin O/FCF, Masson Trichrome, Alcian/PAS and Hematoxylin and Eosin, was also determined, as they are complementary for the histopathological verification of intervertebral disc degeneration. By expressing proteins using the immunohistochemistry technique, it was possible to consider two stages of disc degeneration: cell proliferation with chondrocyte cluster formation, and induction of apoptosis. Conclusion: This study enabled the histological and immunohistochemical characterization to be determined for lumbar degenerative disc disease, and its degrees of evolution, by determining new disc degeneration scores.Keywords: Intervertebral disc; Histology; Immunohistochemistry; Cell proliferation; Apoptosis. RESUMO

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Section: Discussionmentioning

confidence: 75%

Histological Markers of Degeneration and Regeneration of the Human Intervertebral Disk

et al. 2017

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“…67,68 Schell et al transplanted types I and III collagen membrane with bone graft onto the fullthickness defect of the knee joint of sheep, but this resulted in no difference in cartilage repair compared to an empty control defect. 67 Ho et al covered the full-thickness cartilage defect in pig with PCL-collagen electrospun mesh with MSCs. After 6 months, the cartilage defect was partially covered with white reparative tissue.…”

Section: ■ Discussionmentioning

confidence: 99%

“…A few of the studies employed the electrospun mesh or collagen membrane on top of the defect to replace a periosteal flap for repairing cartilage defect. , Schell et al transplanted types I and III collagen membrane with bone graft onto the full-thickness defect of the knee joint of sheep, but this resulted in no difference in cartilage repair compared to an empty control defect . Ho et al covered the full-thickness cartilage defect in pig with PCL-collagen electrospun mesh with MSCs.…”

Section: Discussionmentioning

confidence: 99%

Composite System of PLCL Scaffold and Heparin-Based Hydrogel for Regeneration of Partial-Thickness Cartilage Defects

et al. 2012

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Delivering isolated chondrocytes with matrix is a promising approach to promote the cartilage repair. The present study attempted to combine the advantages of porous scaffold and hydrogel in delivering chondrocytes to partial-thickness cartilage defects. An electrospun, gelatin-incorporated PLCL scaffold mechanically similar to natural cartilage was fabricated, and chondrocytes were seeded using an injectable heparin-based hydrogel for efficient cell seeding. The scaffold/hydrogel composite showed more enhanced expression of chondrogenic genes and production of GAGs than those prepared without hydrogel. In addition, significant cartilage formation showing good integration with surrounding, similar to natural cartilage, was observed by scaffold/hydrogel composite system in partial-thickness defects of rabbit knees while no regeneration was observed in control defects. Although no exogenous chondrogenic factors were added, it was evident that the scaffold/hydrogel composite system was highly effective and better than the scaffold alone system without hydrogel for cartilage regeneration both in vitro and in vivo.

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“…Absent Present detail elsewhere (14). In brief, specimens were fixed in 10% formalin, and dehydrated and embedded in methyl methacrylate, from which 6-mm-thick serial slices were made.…”

Section: Histologymentioning

confidence: 99%

Osteochondral defect repair after implantation of biodegradable scaffolds: indirect magnetic resonance arthrography and histopathologic correlation

Streitparth¹,

Schöttle

Schlichting

et al. 2009

Acta Radiol

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Crushed bone grafts and a collagen membrane are not suitable for enhancing cartilage quality in the regeneration of osteochondral defects—An in vivo study in sheep

Cited by 8 publications

References 45 publications

Histological Markers of Degeneration and Regeneration of the Human Intervertebral Disk

Histological Markers of Degeneration and Regeneration of the Human Intervertebral Disk

Composite System of PLCL Scaffold and Heparin-Based Hydrogel for Regeneration of Partial-Thickness Cartilage Defects

Osteochondral defect repair after implantation of biodegradable scaffolds: indirect magnetic resonance arthrography and histopathologic correlation

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