Crustacean hyperglycemic hormones of Penaeus monodon: cloning, production of active recombinant hormones and their expression in various shrimp tissues

Hyperglycemic activity of the recombinant crustacean hyperglycemic hormone B1 isoform (CHH-B1) of the Pacific white shrimp Litopenaeus vannamei

“…Even though the recombinant CHH-B1 peptide has no additional tags that aid its purification, it was successfully purified by RP-HPLC. This purification strategy has been used before to obtain other bioactive recombinant CHH-family peptides expressed in P. pastoris, for example Pem-CHH1-3, Pem-MIH1 and Pem-GIH from Penaeus monodon [39,40,43,51], and Pej-SGP-III from Penaeus japonicus [28]. At the protein level, the consensus appears to be that the eyestalk predominantly expresses CHH isoforms with C-terminal amidation that show significant induction of hyperglycemia, suppression of ecdysteroid synthesis, or both, whereas the extraeyestalk tissues predominantly express CHH-L isoforms that have a free C-terminus and have generally been reported not to have hyperglycemic activity [2,10,29].…”

Expression and applications of recombinant crustacean hyperglycemic hormone from eyestalks of white shrimp (Litopenaeus vannamei) against bacterial infection

“…Its mRNA was present at a high level in crustacean eyestalks. In previous work, the expression of the CHH gene in P. monodon was not only restricted to the eyestalk but also detectable in the heart and in the gill [14]. In crustacean, neuroendocrine centers are located in different structures of the nervous system and one of these structures, the eyestalk of X-organ-sinus gland complex (XO-SG), is traditionally considered as the major endocrine system of decapods [6,28].…”

“…The sequence of CHH hormone from Carcinus maenus was first reported by Kegel and coworkers [9]. Subsequently, CHH peptides sequences have been described and characterized in lobster [10], crabs [11], crayfishes [12], prawns [13,14] and shrimp [8,15]. CHH functions mainly in the regulation of glucose levels, as well as in the metabolism of carbohydrates and lipids [16].…”

A chimeric recombinant crustacean hyperglycemic hormone fromLitopenaeus schmitti(Burkenroad) obtained as C-terminus fusion protein boost hemolymph glucose concentration inLitopenaeus vannamei(Boone)

“…On the other hand, although the CHH protein has been expressed in a soluble form and biologically active in the yeast expression system, it has been shown that their yield is much less than that obtained in E. coli (Udomkit et al . ). Solutions to the bacterially expressed proteins as insoluble aggregates have been the use of additional solubilization and refolding steps.…”