Crustacean leg regeneration restores complex microanatomy and cell diversity

Almazán, Alba; Çevrim, Çağrı; Musser, Jacob M.; Averof, Michalis; Paris, Mathilde

doi:10.1126/sciadv.abn9823

Cited by 14 publications

(7 citation statements)

References 79 publications

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“…In Parhyale hawaiensis, the regenerated limb appears to be a perfect replica of the original limb before amputation. 10 The size of the newly regenerated limb is the same as that of the normal limb after the second molt in Eriocheir sinensis. 11 In Alpheus angulosus, the regenerated claw differs from mature claws in the first molt, while the most of differences disappeared in the second molt.…”

Section: Introductionmentioning

confidence: 84%

Transcriptome analysis of Litopenaeus vannamei during the early stage of limb regeneration process

Yue,

Yuan,

Jang

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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Regeneration is a process in which organisms regrow new tissues or organs at the injury site, which has attracted the attention of many scientists and nonscientists. However, the underlying molecular mechanisms of regeneration after autotomy are largely unknown. In this study, we conducted RNA-seq sequencing on regenerated limb bud tissues of Litopenaeus vannamei at 0 hours post autotomy (0 hpa), 12 hours post autotomy (12 hpa), and 24 hours post autotomy (24 hpa). A total of 2,192 differentially expressed genes related to energy metabolism, transcription and translation, and epidermis development were identified between 0 hpa and 12 hpa, such as triosephosphate isomerase A, triosephosphate isomerase B, and zinc finger protein 367 that is upregulated in 12 hpa. Between 12 hpa and 24 hpa, 1,447 differentially expressed genes were identified that were related to cuticle development and energy metabolism, such as cuticle protein 6, which is upregulated in 24 hpa, and triosephosphate isomerase is downregulated in 24 hpa. The results indicated that energy metabolism, transcription and translation, epidermal formation, and chitin metabolism processes are involved during the early stage of limb regeneration. This study provides basic knowledge for investigating the molecular mechanisms associated with limb regeneration in crustaceans at the early regeneration stage.

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Section: Introductionmentioning

confidence: 84%

Transcriptome analysis of Litopenaeus vannamei during the early stage of limb regeneration process

Yue,

Yuan,

Jang

et al. 2023

Israeli Journal of Aquaculture - Bamidgeh

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“…One possibility is that the end of regeneration recapitulates the patterning and growth of embryonic organs, where growth ultimately becomes regulated at the organismal level. For example, regeneration in molting animals (e.g., crustaceans) produces a miniature facsimile of the original appendage, which is only capable of additional growth upon subsequent molts [36][37][38] . In zebrafish, caudal fin regeneration is generally consistent with the overall size of the animal, but genetic mutants do exist in which regenerating fins lose their allometry and produce dramatically overgrown fins 39 .…”

Section: Box 3 | Where Do Developmental and Regenerative Processes Mo...mentioning

confidence: 99%

Enduring questions in regenerative biology and the search for answers

Seifert,

Duncan,

Zayas

2023

Commun Biol

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The potential for basic research to uncover the inner workings of regenerative processes and produce meaningful medical therapies has inspired scientists, clinicians, and patients for hundreds of years. Decades of studies using a handful of highly regenerative model organisms have significantly advanced our knowledge of key cell types and molecular pathways involved in regeneration. However, many questions remain about how regenerative processes unfold in regeneration-competent species, how they are curtailed in non-regenerative organisms, and how they might be induced (or restored) in humans. Recent technological advances in genomics, molecular biology, computer science, bioengineering, and stem cell research hold promise to collectively provide new experimental evidence for how different organisms accomplish the process of regeneration. In theory, this new evidence should inform the design of new clinical approaches for regenerative medicine. A deeper understanding of how tissues and organs regenerate will also undoubtedly impact many adjacent scientific fields. To best apply and adapt these new technologies in ways that break long-standing barriers and answer critical questions about regeneration, we must combine the deep knowledge of developmental and evolutionary biologists with the hard-earned expertise of scientists in mechanistic and technical fields. To this end, this perspective is based on conversations from a workshop we organized at the Banbury Center, during which a diverse cross-section of the regeneration research community and experts in various technologies discussed enduring questions in regenerative biology. Here, we share the questions this group identified as significant and unanswered, i.e., known unknowns. We also describe the obstacles limiting our progress in answering these questions and how expanding the number and diversity of organisms used in regeneration research is essential for deepening our understanding of regenerative capacity. Finally, we propose that investigating these problems collaboratively across a diverse network of researchers has the potential to advance our field and produce unexpected insights into important questions in related areas of biology and medicine.

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“…The basic genetic tools have also been established (20). Additionally, an amazing live imaging technique using adhesion has been established in Parhylae (21) and was used in the comparative analysis of leg regeneration and developmental phenomena (22, 23). On the other hand, Daphnia and Parhyale have almost the same morphology as their parents from birth, with few tissues undergoing extreme morphogenesis through ecdysis.…”

Section: Introductionmentioning

confidence: 99%

Post-embryonic tail development through molting of the freshwater shrimpNeocaridina denticulata

Adachi,

Moritoki,

Shindo

et al. 2024

Preprint

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Background: Understanding postembryonic morphogenesis through molting in arthropods has recently become a focus of developmental biology. The hierarchical mechanisms of epithelial sheet folds play a significant role in this process. Drosophila is a well-studied model for holometabolous insects, with extensive research on imaginal disc growth. While developmental processes in other arthropods have been described, live imaging of morphological changes is challenging due to the macroscopic movements and hard cuticles. Neocaridina denticulata, a crustacean, presents unique tail morphogenesis through molting, which makes it the potential model. This study investigated the development of the tail in Neocaridina denticulata through histological analysis and in vivo live imaging using fluorescent probes. This study also performed long-read sequencing of the whole genome for future genetic tools. Results: The tail of Neocaridina was found to undergo two major changes with the first ecdysis. Firstly, the branches of the uropods are cleared, and secondly, the telson undergoes convergent elongation. Cross-sectional analysis revealed that uropod and telson branching occurs immediately after hatching in the form of cuticle branching. The surface structure of the developmental tail suggested that telson elongation is achieved by the extension of anisotropic furrows in the cuticle during ecdysis. Anisotropy of cuticle furrows was associated with the epithelial cell shape, and the anisotropy of cell shape was found to occur during development from post-hatching. We also established an in vivo live imaging system with UV-LED resin and detected the changes of tail development over time. in vivo live imaging analysis revealed that telson contraction occurs gradually prior to ecdysis. Furthermore, we have also provided a draft genome of Neocaridina. Conclusion: Neocaridina denticulata is a valuable model for studying morphogenesis in arthropods through molting. The tail undergoes complex changes involving cuticle branching, anisotropic furrows, and cellular dynamics. in vivo live imaging system provides insights into the developmental process, and the draft genome enhances the potential for genetic tools in future studies. This research contributes to the understanding of arthropod morphogenesis and provides a foundation for further developmental and cytological investigations in Neocaridina.

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Crustacean leg regeneration restores complex microanatomy and cell diversity

Cited by 14 publications

References 79 publications

Transcriptome analysis of Litopenaeus vannamei during the early stage of limb regeneration process

Transcriptome analysis of Litopenaeus vannamei during the early stage of limb regeneration process

Enduring questions in regenerative biology and the search for answers

Post-embryonic tail development through molting of the freshwater shrimpNeocaridina denticulata

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