Cry toxins of Bacillus thuringiensis: a glimpse into the Pandora’s box for the strategic control of vector borne diseases

Rao, Priyashi; Goswami, Dweipayan; Rawal, Rakesh

doi:10.1007/s42398-020-00151-9

Cited by 11 publications

(5 citation statements)

References 86 publications

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“…Mosquitoes are the vectors that spread infectious diseases to various life forms including humans. Many of these mosquito vectors are bloodsucking macroscopic flying creatures that ingest disease-causing pathogens during a blood meal from an infected host (human or animal) and then spread them to a new host, resulting in a chain of severe life-threatening infections 2 . When a vector (like mosquito) gets infected, it is also capable of spreading the pathogen for the entirety of its life onto each subsequent bite/blood meal.…”

Section: Revealing the Molecular Interplay Of Curcumin As Culex Pipie...mentioning

confidence: 99%

Revealing the molecular interplay of curcumin as Culex pipiens Acetylcholine esterase 1 (AChE1) inhibitor

Rao

Goswami

Rawal

2021

Sci Rep

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Emergence of vector borne diseases has continued to take toll on millions of lives since its inception. The use of insecticides began as vector control strategy in the early 1900’s but the menace of insects is still prevalent. Additionally, the inadequate use of organophosphates and carbamates which target acetylcholine esterase (AChE), are known to develop resistance amongst vectors of transmission and are toxic to humans. In this study, extensive computational screening was performed using homology modelling, molecular docking, molecular dynamics (MD) simulation and free energy change calculation, which highlighted curcumin as a lead molecule out of ~ 1700 phytochemicals against Culex pipiens AChE. In vivo larvicidal activity was carried out along with in vivo and in vitro AChE inhibition assay to determine the biochemical efficacy of curcumin. Our study reveals that curcumin induces mortality in Cx. pipiens at an early stage of its life cycle by AChE inhibition. This also underlines the use of curcumin as a coming-age natural product insecticide.

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Section: Revealing the Molecular Interplay Of Curcumin As Culex Pipie...mentioning

confidence: 99%

Revealing the molecular interplay of curcumin as Culex pipiens Acetylcholine esterase 1 (AChE1) inhibitor

Rao

Goswami

Rawal

2021

Sci Rep

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“…Mosquitoes are known to transmit the deadliest diseases known to mankind such as chikungunya, dengue, filariasis, malaria, yellow fever, West Nile virus, etc. Every year 700 million people in Africa, Central and South America, Mexico, Russia, and most of Asia are affected and approximately two million deaths from diseases that are spread by mosquitoes [ 37 ]. To control the outgrowth of mosquitoes several vector control strategies have been employed, of which the most prominent strategy is to make use of chemical insecticides and this strategy is being used since 1800s where development of insecticides such as organochlorine compounds, organophosphates, carbamates, pyrethroids and formamidines, etc, was envisaged.…”

Section: Discussionmentioning

confidence: 99%

Extending the lore of curcumin as dipteran Butyrylcholine esterase (BChE) inhibitor: A holistic molecular interplay assessment

2022

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Since its origin, the emergence of vector-borne infections has taken a toll on incalculable human lives. The use of chemical insecticides is one of the early known methods of vector control and although their use is still a prevalent way to combat insect population sadly the perils of insects related transmission still persists. Most commonly, the existing insecticides face the wrath of getting resisted repeatedly, paying way to develop resilient, efficient, and cost-effective natural insecticides. In this study, computational screening was performed using homology modelling, E-pharmacophore feature mapping, molecular docking, Density Function Theory (DFT) assessment, Molecular mechanics generalized Born surface area (MM-GBSA) based binding free energy calculations and Molecular Dynamics (MD) simulation to identify a potential lead phytochemical out of a manually curated library from published literature. The protein target used under this study is insect Butyrylcholine esterase (BChE). Additionally, in vitro insect (Aedes aegypti) BChE inhibition assay was also performed with the top phytochemical identified from in silico assessments. Our research highlights that curcumin leads to inhibition of enzyme BChE of Ae. aegypti. The identified mode of action of curcumin as an insect BChE inhibitor indicates the possibility of its use as an environment friendly and natural futuristic insecticide.

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“…Controlling vectors carrying mosquito larvae OPEN ACCESS EDITED BY is the only way to stop the spread of these illnesses. Often, this is achieved by consistently using larvicidal pesticides, particularly insect growth inhibitors and organophosphates (Rao et al, 2021). The frequent application of these chemical sprays has negative effects on populations that are not the intended target and may even cause resistance strain breakout (Zaller and Zaller, 2020).…”

Section: Introductionmentioning

confidence: 99%

Mushroom tyrosinase enzyme catalysis: synthesis of larvicidal active geranylacetone derivatives against Culex quinquesfasciatus and molecular docking studies

Mullaivendhan,

Ahamed,

Arif

et al. 2024

Front. Chem.

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The grindstone process, which uses tyrosinase as a catalyst, was used to create analogues of geranylacetone. Tyrosinase was used to prepare the Mannich base under favourable reaction conditions, resulting in a high yield. All synthesized compounds were characterized using FTIR, Nuclear magnetic resonance, and mass spectral analyses. The active geranylacetone derivatives (1a-l) were investigated for larvicidal activity against Culex quinquefasciatus; compound 1b (LD50:20.7 μg/mL) was noticeably more effective than geranylacetone (LD50: >100 μg/mL) and permethrin (LD50: 24.4 μg/mL) lead compounds because of their ability to kill larvae and use them as pesticides. All compounds (1a-1l) were found to be low toxic, whereas compounds 1b, 1d, and 1k were screened for antifeedant screening of non -aquatic target for the toxicity measurement against marine fish Oreochromis mossambicus at 100 μg/mL caused 0% mortality in within 24 h. Molecular docking studies of synthesised compound 1b and permethrin docked with 3OGN, compound 1b demonstrated a greater binding affinity (−9.6 kcal/mol) compared to permethrin (−10.5 kcal/mol). According to these results, the newly synthesised geranylacetone derivatives can serve as lead molecules of larvicides agents.

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Cry toxins of Bacillus thuringiensis: a glimpse into the Pandora’s box for the strategic control of vector borne diseases

Cited by 11 publications

References 86 publications

Revealing the molecular interplay of curcumin as Culex pipiens Acetylcholine esterase 1 (AChE1) inhibitor

Revealing the molecular interplay of curcumin as Culex pipiens Acetylcholine esterase 1 (AChE1) inhibitor

Extending the lore of curcumin as dipteran Butyrylcholine esterase (BChE) inhibitor: A holistic molecular interplay assessment

Mushroom tyrosinase enzyme catalysis: synthesis of larvicidal active geranylacetone derivatives against Culex quinquesfasciatus and molecular docking studies

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