Cryo-Electron Microscopy and Ultramicrotomy: Reminiscences and Reflections

Fernández-Morán, H.

doi:10.1016/b978-0-12-014578-2.50012-6

Cited by 8 publications

(9 citation statements)

References 74 publications

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“…This is what Cryo‐EM Of Vitreous Sections (CEMOVIS) does. The idea dates from the late 1950s (Fernandez‐Moran, 1985) and our own involvement has been continuous since the beginning of our cryo‐EM effort. The start was promising.…”

Section: Cemovismentioning

confidence: 99%

Cryo-electron microscopy of vitreous sections

Al‐Amoudi

Chang

Leforestier

et al. 2004

EMBO J

437

274

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Since the beginning of the 1980s, cryo-electron microscopy of a thin film of vitrified aqueous suspension has made it possible to observe biological particles in their native state, in the absence of the usual artefacts of dehydration and staining. Combined with 3-d reconstruction, it has become an important tool for structural molecular biology. Larger objects such as cells and tissues cannot generally be squeezed in a thin enough film. Cryo-electron microscopy of vitreous sections (CEMOVIS) provides then a solution. It requires vitrification of a sizable piece of biological material and cutting it into ultrathin sections, which are observed in the vitrified state. Each of these operations raises serious difficulties that have now been overcome. In general, the native state seen with CEMOVIS is very different from what has been seen before and it is seen in more detail. CEMOVIS will give its full potential when combined with computerized electron tomography for 3-d reconstruction

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Section: Cemovismentioning

confidence: 99%

Cryo-electron microscopy of vitreous sections

Al‐Amoudi

Chang

Leforestier

et al. 2004

EMBO J

437

274

View full text Add to dashboard Cite

show abstract

“…The vast majority of the eukaryote cell volume exceeds the accessible specimen thickness. Therefore, vitreous sectioning techniques have been developed to overcome these problems and considerable efforts were undertaken to establish cryo-ultramicrotomy as a routine method (Dubochet et al, 1988;Fernandez-Moran, 1985).…”

Section: Introductionmentioning

confidence: 99%

Micromachining tools and correlative approaches for cellular cryo-electron tomography

Rigort

Bäuerlein

Leis

et al. 2010

Journal of Structural Biology

238

224

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“…Por avatares de la política, Fernández-Morán, quién había sido nombrado Ministro de Educación en los últimos días del gobierno de Pérez Jiménez, emigró entonces a los Estados Unidos donde continuó su carrera científica en varias instituciones (Hospital General de Massachusetts, el Instituto Tecnológico de Massachusetts y la Escuela de Medicina de la Universidad de Chicago). Esos fueron años de gran productividad científica, cuando dirigió su atención al estudio de las estructuras subcelulares y a mejorar las técnicas y el instrumental usado para la microscopia electrónica (7)(8)(9)(10)16). A partir de 1964, y especialmente después de 1983, Fernández-Morán comenzó a visitar Venezuela con cierta frecuencia, aunque sin establecer contactos de trabajo permanentes con la comunidad científica del país.…”

Section: Esbozo Biográfico De Humberto Fernández-moránunclassified

“…Es así como a finales de 1946 Fernández-Morán se incorpora como asistente extranjero en la clí-nica de Olivecrona. No tenemos información sobre posibles actividades médicas o quirúrgicas de Fernández-Morán en dicha clínica, aunque el relata que las actividades de postergado en neurología y neurocirugía absorbían toda su energía (16). Casi con seguridad Fernández-Morán no practicó psicocirugías en Estocolmo, ya que estas estaban limitadas a los hospitales psiquiátricos de ese país (31).…”

Section: Fernández-morán Viaja a Suecia Para Completar Su Entrenamiento Y Cambia Su Interés Profesionalunclassified

Las lobotomías practicadas en 1946 por Humberto Fernández-Morán en Maracaibo, Venezuela, o cómo un joven médico decidió dedicarse a la investigación científica básica

Esparza

2018

Invest Clin

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Humberto Fernández-Morán (1924-1999 was one of the Venezuelan researchers with greater international projection during the second half of the twentieth century, especially because of his studies on the cellular ultrastructure, particularly of the nervous tissue, and for his contributions to development of electron microscopy. In this article we discuss a little-known period of Fernández-Morán, from mid-1944, when he returned to Venezuela after completing his medical studies in Germany, until he marched to Sweden at the end of 1946, where he completed his scientific training. We propose that during this period, Fernández-Morán became interested in neuropsychiatry under the influence of Carlos Ottolina in Caracas. After completing a training in Washington under the direction of Walter Freeman, who was the "champion" of transorbital lobotomy for the treatment of mental illnesses, Fernández-Morán returned to Maracaibo where he practiced, with great dexterity, 25 transorbital lobotomies in the Psychiatric Hospital of Maracaibo. He then traveled to Stockholm, Sweden, as a "Research Fellow" in one of the best neurosurgery centers in the world, under the direction of Hebert Olivecrona. It is then that Fernández-Morán, moved by the absolute futility of seeing patients die with brain tumors, and incentivized by Olivecrona, decides to devote himself to basic research. He then joined the group of Manne Siegbahn, who was the director of the Nobel Institute of Physics and who had designed an electronic microscope of his own invention. In that laboratory and with that electron microscope, Fernández-Morán began his research career.

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Cryo-Electron Microscopy and Ultramicrotomy: Reminiscences and Reflections

Cited by 8 publications

References 74 publications

Cryo-electron microscopy of vitreous sections

Cryo-electron microscopy of vitreous sections

Micromachining tools and correlative approaches for cellular cryo-electron tomography

Las lobotomías practicadas en 1946 por Humberto Fernández-Morán en Maracaibo, Venezuela, o cómo un joven médico decidió dedicarse a la investigación científica básica

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