2021
DOI: 10.1016/j.molcel.2021.06.001
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Cryo-EM structure of the periplasmic tunnel of T7 DNA-ejectosome at 2.7 Å resolution

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Cited by 31 publications
(43 citation statements)
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“…Podoviridae-like viruses have only a minimalist tail but deploy a channel in situ upon attachment that acts as an ejectosome. The DNA is actively pulled out of the particle by the ejectosome itself and the bacterial RNA polymerase [32]. Siphoviridae-like tails have a diverse range of tail tip structures which are employed in the penetration of their host [19,[45][46][47][48].…”
Section: Phage Tails As Cell Puncturing Devicesmentioning
confidence: 99%
“…Podoviridae-like viruses have only a minimalist tail but deploy a channel in situ upon attachment that acts as an ejectosome. The DNA is actively pulled out of the particle by the ejectosome itself and the bacterial RNA polymerase [32]. Siphoviridae-like tails have a diverse range of tail tip structures which are employed in the penetration of their host [19,[45][46][47][48].…”
Section: Phage Tails As Cell Puncturing Devicesmentioning
confidence: 99%
“…Four subunits related by four-fold rotational symmetry are visible in the procapsid [30] and maturevirion [28,29,31] reconstructions, although more subunits that do not obey strict rotational symmetry could be present inside the head and invisible to cryo-EM analysis. Only the N-terminal portion of gp16, named gp16-N (residues 1-228), was detected at high resolution in the post-ejection conformation (Figure 1B) [55], whereas gp16-C was visualized at low resolution in the lipid nanodiscs [55] and by cryo-ET [36], preventing direct comparison of the pre-ejection state that was visualized in situ in the mature T7 capsid [31]. We used all of the structural and biochemical information available in the literature to generate a composite model of the full-length gp16 in the post-ejection conformation (Figure 3C).…”
Section: Conformational Gymnastics Of T7 Ejection Proteinsmentioning
confidence: 99%
“…Recent in vitro studies have characterized the composition of the T7 ejection proteins and elucidated the high-resolution cryo-EM structures of gp15 and an N-terminal portion of gp16 (gp16-N) [ 55 , 56 ], which form a hexameric tunnel wide enough for dsDNA to pass through, bridging the outer membrane (OM) with the inner membrane (IM). In parallel, a recent high-resolution structure of the mature T7 virion [ 31 ] elucidated the atomic structures of the T7 ejection proteins in situ, arranged onto the portal protein.…”
Section: The T7 Dna Ejectosomementioning
confidence: 99%
“…This protocol was used for high-resolution cryo-EM structure analysis of the T7 periplasmic tunnel and can be adapted to study ejection proteins from other phages. For complete details on the use and execution of this protocol, please refer to Swanson et al. (2021) .…”
mentioning
confidence: 99%
“…For complete details on the use and execution of this protocol, please refer to Swanson et al. (2021) .…”
mentioning
confidence: 99%