Cryobanking of Banana (Musa Sp.) Germplasm in India: Evaluation of Agronomic and Molecular Traits of Cryopreserved Plants

Agrawal, AP; Tyagi, Rishi; Goswami, R. K.; Uma, S.; Saraswathi, M.; Durai, P.

doi:10.17660/actahortic.2011.908.14

Cited by 6 publications

(3 citation statements)

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“…Various studies reported no instability in the molecular analysis of cryopreserved plants [62][63][64][65]. SSR markers are used to determine genetic changes within one species and to identify the somaclonal variability.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation and Cryotolerance Mechanism in Zygotic Embryo and Embryogenic Callus of Oil Palm

Wei

Shi

Khan

et al. 2023

Forests

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Oil palm (Elaeis guineensis) is the highest oil-yielding commercially grown perennial tree. Oil palm germplasm conservation and in vitro clonal propagation strengthened the world’s efforts to ensure future food security. Cryopreservation provides long-term storage for germplasm. The storage of plant material at cryogenic temperatures (−196 °C) following dehydration causes cryoinjury. The cryotolerance mechanism has rarely been studied in oil palm zygotic embryos (ZE) and embryogenic calli (EC). A simple and effective cryopreservation method was established for ZE. ZE surrounded by endosperm was air-dried for 3 days without any complicated chemical pre-treatments before cryopreservation, while the viability rate and following germination rate could reach up to 96.67% and 90.88%, respectively. As for EC, the preferred method could be pre-culture in liquid MS medium with 0.3 M sucrose for 12 h and PVS2 treatment for 5 min prior to cryopreservation, and the viability rate reached 68.33%. SSR markers were used to verify the genetic stability after cryopreservation. In addition, changes in enzyme activities (CAT, POD, and SOD) showed a consistent trend with H2O2 production among ZE samples, indicating that these antioxidants were involved in ROS scavenging. Furthermore, differently expressed genes (DEGs) related to ROS, osmotic, and cold stress responses were selected for correlation network analysis. Most genes involved in ROS production (RBOH, PAO, and PRX) and ROS scavenging (APX, PER, SOD, CAT, GPX, and AOX) showed higher expression levels in EC, suggesting that EC was more sensitive to oxidative stress than ZE. The cryotolerance mechanism was further summarized accordingly. These results contributed to cryopreservation methods and provided a better understanding of cryotolerance in oil palm.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation and Cryotolerance Mechanism in Zygotic Embryo and Embryogenic Callus of Oil Palm

Wei

Shi

Khan

et al. 2023

Forests

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“…Hence, before routinely establishing the cryobank any species, studies on the field performance and genetic fidelity of plants derived from cryopreserved plant material need to be carried out. Agrawal et al, (2009) compared the performance of cryopreserved banana germplasm of cultivar 'Sommarani Monthan' (AAB, Monthan subgroup) for agronomic and molecular traits (using simple sequence repeats; SSR) with that of its parental type. Experiment included cryopreserved germplasm, cryopreservation control, in vitroconserved plants, and natural sucker raised plants.…”

Section: Stability Of Cryopreserved Materialsmentioning

confidence: 99%

Cryopreservation of Fruit Genetic Resources-A Review

Anushma¹,

Dhanyasree²,

Rafeekher³

2020

Int.J.Curr.Microbiol.App.Sci

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Conserving the plant genetic resources through any means is of utmost importance to prevent the loss of plant biodiversity. Long-term storage using liquid nitrogen (LN) is usually adopted for developing long-term base collections of crops for use in the distant future. The fruit crops are commonly propagated through vegetative means and are majorly conserved as ex situ field gene banks, which require large areas and demand intensive field management. The need for repeated reculturing and chances of contamination during subcultures in in vitro banking necessitate a long-term contamination free conservation through cryobanking. Improved cryopreservation techniques will provide added security in the preservation of important woody plant germplasm formerly preserved only as field collections.

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“…They are the cheapest, plentiful and the most nourishing of all the fruits and are consumed by the rich and poor alike. The plants are considered as a symbol of prosperity and fertility due to its place as a token of goodwill in various religious practices and ceremonial functions (Agrawal et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Allelopathic interactions of certain Musa cultivars against Odoiporus longicollis (Olivier)

Kavitha,

Murugan,

Evans

2015

Entomon

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Odoiporus longicollis is a major pest of the banana (Musa) cultivars that enormously feeds on the pseudostem and causes serious damage to banana cultivation. It is a monophagous pest of banana, which showed extreme preference to some commercially viable cultivars such as Nendran, (AAB) Palayankodan,(AAB) Red banana (AAA) and extreme non preference to some commercially non viable cultivars such as Kadali, Kannan, Aattinkombu (all AA types) and Thenkaali (AAB). Field study, diversity analysis and pest status of Musa cultivars in Chittar panchayt of Pathanamthitta district (Kerala) by Arc GIS software showed that maximum diversity of cultivars with minimum pest attack was seen in those wards which are ecotones with respect to forest and agro ecosystem. Rearing of O.longicollis larvae in Thenkaali and Aattinkombu has resulted mortality of them evidenced by hyperprotenemia and hyperuricemia of haemolymph. The HPTLC study has revealed that pseudostem of Aattinkombu and Thenkaali possessed three additional compounds which were not present in pest sensitive cultivars. The differential distribution of secondary metabolites in the pseudostem of the above two cultivars can also be felt by difference in the smell of freshly cut pseudostem.

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Cryobanking of Banana (Musa Sp.) Germplasm in India: Evaluation of Agronomic and Molecular Traits of Cryopreserved Plants

Cited by 6 publications

References 0 publications

Cryopreservation and Cryotolerance Mechanism in Zygotic Embryo and Embryogenic Callus of Oil Palm

Cryopreservation and Cryotolerance Mechanism in Zygotic Embryo and Embryogenic Callus of Oil Palm

Cryopreservation of Fruit Genetic Resources-A Review

Allelopathic interactions of certain Musa cultivars against Odoiporus longicollis (Olivier)

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