1981
DOI: 10.3109/10409238109108701
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Cryoenzymolog

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1983
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Cited by 50 publications
(22 citation statements)
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“…Many biological enzymes show a simple Arrhenius trend for enzymatic activity near room or physiological tempera-ture but break from this trend at low temperatures, i.e., they have a limited thermal dynamic range (33). We propose that the architecture of cytoplasmic dynein allows for tuning of this dynamic range between species.…”
Section: Discussionmentioning
confidence: 94%
“…Many biological enzymes show a simple Arrhenius trend for enzymatic activity near room or physiological tempera-ture but break from this trend at low temperatures, i.e., they have a limited thermal dynamic range (33). We propose that the architecture of cytoplasmic dynein allows for tuning of this dynamic range between species.…”
Section: Discussionmentioning
confidence: 94%
“…However, the choice of solvents for biopolymers is usually limited to mixtures of water with selected alcohols (28,29), concentrated solutions of sugars (30,31) or salts (23), because most common organic solvents unfold or precipitate proteins and inactivate enzymes. For proteins the most popular cryosolvents are binary mixtures of water with ethylene glycol or glycerol, although methanol (32,33), dimethyl sulfoxide, and dimethylformamide (32), as well as ternary mixtures of water with combinations of these solvents (29) also can be used. To prevent crystallization of water upon freezing, 50 – 70% w/w fractions of organic solvent are used in cryospectroscopic studies.…”
Section: Introductionmentioning
confidence: 99%
“…For instance, FTIR study of water-glycerol mixtures at low temperatures indicated that phase separation and crystallization of water is fully suppressed at 65 % w/w (60% v/v) fraction of glycerol (34). Fortunately, in most cases enzymes remain folded and active in such mixed cryosolvents even at extremely low temperatures (32,33,35-38) despite the very well-known phenomenon of ‘cold denaturation’ (39,40). …”
Section: Introductionmentioning
confidence: 99%
“…The lowtemperature studies of enzymes, cryoenzymology, as well as low-temperature crystallography have necessitated the use of organic solvents in these protein structural studies (2). In addition, many enzyme-catalyzed reactions are also being investigated in organic media to enhance the industrial applications, specifically to take advantage of the high selectivity and specificity of the enzyme (3).…”
mentioning
confidence: 99%