Cryogenic Q-band (35GHz) probehead featuring large excitation microwave fields for pulse and continuous wave electron paramagnetic resonance spectroscopy: Performance and applications

Asymmetry in the Homodimeric ABC Transporter MsbA Recognized by a DARPin

“…DEER traces were measured in a home-made high power Q band spectrometer equipped with a home-made oversized resonator (36). All pulse lengths were set to 12 ns and deuterium modulation artifacts were averaged (8 times d1 time increment of 16 ns).…”

Cryogenic 35GHz pulse ENDOR probehead accommodating large sample sizes: Performance and applications

“…All three parts of the resonator (aluminum block, cover plate and iris-coupler plate) are coated with 2 lm of silver (Collini-Flühmann AG, 8600 Dübendorf, Switzerland) and have the outside dimensions of the WR 28 flange. The iris-coupler plate, whose construction is detailed in reference [12], terminates the wave guide (2) in a 2.8 Â 7.0 Â 3.0 mm round-shaped groove that has been carved in the plate. The groove is opened in the middle to the resonator space through a 3 Â 1 mm slot along the y-axis (iris).…”

“…Recently we showed that the application of loop-gap resonators (LGR) at 35 GHz with high filling factors, matched for particular sample sizes, lead to optimum mw B 1 -field strengths in the sample area [12]. It has also been shown that strong electron spin echo (ESE) signals are generated at 35 GHz resonance frequencies using large sample sizes with TE 011 cylindrical cavities [8,[13][14][15].…”

Coordination and binding geometry of methyl-coenzyme M in the red1m state of methyl-coenzyme M reductase

“…The mw pulse lengths were t p/2 = 20 ns and t p = 16 ns, starting times t 10 = t 20 = 96 ns, and a time increment of Dt = 20 ns (data matrix 350 9 350) was used. Q-band Matched-HYSCORE spectra [36,37] were measured with a loop gap resonator [38] at 30 K using a repetition time of 1 ms and the sequence p/2-s-t m -t 1 -p-t 2 -t m -s-echo. The mw pulse lengths were t p = t p/2 = 16 ns, with matched pulses of length t m = 46 ns (mw field strength x 1 /2p = 83.3 MHz), starting times for t 1 and t 2 of 96 ns, a time increment of Dt = 12 ns (data matrix 256 9 256), and s = 108 ns.…”