Cryoloop vitrification of rabbit oocytes

Cai, X.Y.; Chen, G.A.; Lian, Ying; Zheng, Xiaoyun; Peng, Hong-Li

doi:10.1093/humrep/deh805

Cited by 52 publications

(34 citation statements)

References 33 publications

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“…Until now, vitrification has been adopted to cryopreserve embryos [3,4], oocytes [5][6][7][8][9] and ovarian tissue [10][11][12][13]. Kuwayama reported that vitrification, compared with slow cooling, resulted in high survival rates for all stages of embryo development [14].…”

Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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Purpose The aim of this experiment is to detect effects of varying levels of sucrose on vitrified ovarian tissues. Methods Ovarian tissues of mice were vitrified-thawed. Mice were randomly assigned to the fresh control group and experimental groups. According to different concentration of sucrose in vitrification solution, the experimental groups were randomly divided into Group I (0.2 M sucrose), Group II (0.4 M sucrose), Group III (0.8 M sucrose) and Group IV (1.6 M sucrose). Cytology was followed throughout the oophorectomy and transplantation period. Hormone levels and density of follicle were measured 1 month after transplantation. Results The number of days before the resumption of estrous cycles in control group was significantly smaller than those in all of experimental groups. The serum estradiol levels of mice and the follicular density of ovarian grafts in control group were significantly higher than those in all of experimental groups. In addition, the number of days before the resumption of estrous cycles in Group II and Group III were smaller than those in Group I and Group IV. The serum estradiol levels of mice and the follicular density of ovarian grafts in Group II and Group III were significantly higher than those in Group I and Group IV. However, no difference was observed in the number of days before the resumption of estrous cycles and the serum estradiol levels between Group II and Group III. A similar follicular density was also observed in Group II and Group III.Conclusion Sucrose concentration of 0.4 M or 0.8 M in cryoprotective media is suitable for vitrifying mouse ovarian tissues.

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Section: Introductionmentioning

confidence: 99%

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Zhang

Yao

et al. 2009

J Assist Reprod Genet

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“…The evaporative cooling causes the nitrogen to partially solidify, thus creating a nitrogen slush. Samples immersed in nitrogen slush cool more rapidly because they come into contact with liquid nitrogen sooner than those immersed in normal liquid nitrogen (Cai et al, 2005). The Vit Master vitrification machine can provide a very high cooling rate (up to 135,000 • /min).…”

Section: Innovative Vitrification Devicesmentioning

confidence: 99%

Technologies for Cryopreservation: Overview and Innovation

Lopez¹,

Cipri²,

Naso³

2012

Current Frontiers in Cryobiology

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“…An important advantage of the use of nylon loop over conventional vitrification procedures is that the open system lacks any thermo-insulation layer; this, coupled with the small volume (< 1 µl) of vitrification solution, results in both rapid and uniform heat exchange during cooling (Lane et al, 1999a, b;Reed et al, 2002;Cai et al, 2005;Sheehan et al, 2006;Mukaida and Takahashi, 2008). By decreasing the volume of the cryoprotective solution we are able to increase dramatically the freezing speed and decrease the toxicity and osmotic side effects of the CPA (Pogorelov et al, 2006;Katkov and Pogorelov, 2007;Pogorelov et al, 2007).…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

“…Vitrification using the nylon loop/cryoloop was previously used to successfully vitrify human, hamster, mouse, bovine and horse embryos as well as human, horse and bovine oocytes Lane et al, 1999a, b;Oberstein et al, 2001;Reed et al, 2002;Hochi et al, 2004;Cai et al, 2005;Sheehan et al, 2006;Mukaida and Takahashi, 2008;Liebermann and Tucker, 2008). Lane et al (1999b) vitrified mouse and human blastocysts in a cryoprotective solution containing DMSO, EG, F and S with the cryoloop procedure and assessed subsequent development.…”

Section: Acta Veterinaria Hungarica 57 2009mentioning

confidence: 99%

Vitrification of cleavage stage mouse embryos by the cryoloop procedure

Klambauer

Keresztes

Kanyó³

et al. 2009

Acta Veterinaria Hungarica

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By decreasing the volume of the cryoprotective solution it is possible to increase dramatically the freezing speed and -at the same time -reduce the toxicity and osmotic side effects of cryoprotectants (CPA). The objective of our study was to vitrify Day-3 cleavage stage mouse embryos (n = 229) with the cryoloop technology using a new composition of vitrification media. Embryos were exposed to a 2-step loading of CPA, ethylene glycol (EG) and propylene glycol (PG), before being placed on the surface of a thin filmy layer formed from the vitrification solution in a small nylon loop, then they were rapidly submerged into liquid nitrogen. After warming, the CPA was diluted out from the embryos by a 3-step procedure. Survival of embryos was based on morphological appearance after thawing and continued development to expanded blastocysts upon subsequent 48-hour culture. Embryos of the two control groups were either treated likewise except that they were not vitrified, or cultured in vitro without any treatment. Our data show that a high percentage of embryos survived (92.7%) vitrification in the mixture of EG and PG combined with cryoloop carrier and developed normally (89.1%) in vitro after thawing. To our knowledge this is the first report of the successful vitrification of cleavage stage mouse embryos using VitroLoop vitrification procedure.

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Cryoloop vitrification of rabbit oocytes

Abstract: The fastest cooling rate in combination with EG and DMSO as cryoprotectants had the fewest adverse effects on the spindle configuration of rabbit oocytes and embryo development.

Cited by 52 publications

References 33 publications

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Sucrose affecting successful transplantation of vitrified-thawed mouse ovarian tissues

Technologies for Cryopreservation: Overview and Innovation

Vitrification of cleavage stage mouse embryos by the cryoloop procedure

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