Cryomold: A Device for Tissue Embedding in Mohs Micrographic Surgery

Leshin, Barry; Cook, Susan R.; Frye, Debbie W.

doi:10.1111/j.1524-4725.1991.tb03636.x

Cited by 19 publications

(23 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, advantages of the use of specialized tissue embedding devices have been described. 10,11 Franks 12 has put forth the advantages of the use of a machine for tissue mounting. Gross and Field 13 demonstrated that office surgery can closely approximate in hospital surgery with regards to the use of a pathologist in interpreting frozen section specimens.…”

mentioning

confidence: 99%

Mohs Surgery and Processing: Novel Optimizations and Enhancements

Weber

Moody

Dryden

et al. 2000

Dermatologic Surgery

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Mohs Surgery and Processing: Novel Optimizations and Enhancements

Weber

Moody

Dryden

et al. 2000

Dermatologic Surgery

View full text Add to dashboard Cite

show abstract

“…[1][2][3][4]. There are also several papers describing less commonly used methods such as the cryomold [5], Miami special [6], special discs [7], and gradual freezing with liquid nitrogen [8]. Motley and Holt [9] published results from a survey of the members of the American College of MOHS Micrographic Surgery and Cutaneous Oncology, with data on the methods used for freezing tissue.…”

Section: Discussionmentioning

confidence: 98%

Tissue preparation for MOHS’ frozen sections: a comparison of three techniques

Bakhtar

Davidson

et al. 2007

Virchows Arch

View full text Add to dashboard Cite

Accurate, efficient frozen section analysis is important for tumor control. A few studies address the technical issues. More are needed, especially as new technologies become available. The objective of this study is to compare the efficiency of three techniques of flattening tissue for microscopically oriented histologic surgery (MOHS): conventional frozen sectioning, Cryocup, and CryoHist. Conventional chuck/heat sink-frozen section preparation were compared with Cryocup and CryoHist to determine the most efficient technique to examine 100% of the surgical margin of 4-cm diameter, full thickness, fresh autopsy cylinders of anterior abdominal skin, which were marked on their deep and peripheral margins. The specimens were frozen sectioned at 5 microm until all the marking dye was gone from the deep surface, and 95% of the perimeter epidermis could be seen. The conventional chuck required an average of 304 micrometers to clear the deep margin and four fifths did not contain 95% of the epidermal margin. The Cryocup required an average of 284 microm to examine the deep margin and 95% of the epidermal margin. The CryoHist required an average of 104 microm to examine the deep margin and 95% of the epidermal border. The new techniques improve the efficiency and presumably the accuracy of tumor margin analysis.

show abstract

“…Gormley, 11 using an ingenious machine of his own design, achieved complete margins in 96% of samples after cutting into the tissue within a range of 280–980 μm, with a mean depth of 434 μm. Leshin et al, 12 using Tissue Tech cryomolds and a Tissue‐Tek II cryostat (Miles Inc., Elkhart, IN), achieved a mean distance of 305 μm in a range of 10–600 μm. Both of these efforts show that it is possible to get complete margins early in the sectioning process.…”

Section: Discussionmentioning

confidence: 99%

“…There are a variety of methods for flattening specimens and numerous techniques for mounting them onto chucks 2–10 . Several custom devices address these issues, 11–13 but a hint of an ongoing problem can be found in most modern cryostats. The chuck holders in these machines are adjustable.…”

mentioning

confidence: 99%