Cryopreservation affects the quality of in vitro produced bovine embryos at the molecular level

Stinshoff, H.; Wilkening, S.; Hanstedt, A.; Brüning, K.; Wrenzycki, C.

doi:10.1016/j.theriogenology.2011.06.013

Cited by 65 publications

(36 citation statements)

References 45 publications

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“…Here, we did not observe differences in reexpansion rates at different time points as previously reported after use of different vitrification protocols [6,45]. The novel finding of this study is that pre-IVM treatment with caffeine before IVM enhanced blastocyst quality after vitrification and warming as reported by similar hatching rates compared with nonvitrified embryos and superior hatching rates compared with the standard treatment.…”

Section: Discussionsupporting

confidence: 83%

“…The total number of cells was lower in vitrified blastocysts derived from both, standard, and caffeine-treated oocytes, compared with their fresh counterparts, thus confirming the negative effects of cryopreservation on embryo quality [6,7]. Although the number of dead cells in all treatment groups was similar to previous reports [6,45], it was lower in vitrified and warmed blastocysts derived from caffeine-treated oocytes compared with their fresh and vitrified counterparts derived from the standard treatment, indicating improved cell characteristics. Presumably, the positive effects of caffeine during pre-IVM discussed previously have alleviated spontaneous maturation leading to better oocyte quality and therefore improved blastocyst quality.…”

Section: Discussionsupporting

confidence: 80%

“…Despite intensive research efforts around the globe, this situation did not change significantly during the past decades [2][3][4]. The observed molecular, structural, and morphological differences found between in vitro produced embryos and their in vivo produced counterparts are thought to be involved in the increased susceptibility to freezing and thawing [5][6][7]. Several approaches have been made to minimize freezing-related damage and to improve embryo survival after cryopreservation but met with limited success [8].…”

Section: Introductionmentioning

confidence: 99%

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Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

et al. 2016

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Section: Discussionsupporting

confidence: 83%

Section: Discussionsupporting

confidence: 80%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

et al. 2016

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“…We infer that the differences could be linked directly with the development stage of the embryo, volume and concentration of the cryoprotectants. It seems that these factors have a severe impact on embryo cryopreservation (Rios et al, 2010;Shirazi et al, 2010;Stinshoff et al, 2011).…”

Section: Zhaomentioning

confidence: 99%

Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos

Reyes

Jaramillo

2016

Rev Colomb Cienc Pecu

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Facultad de Ciencias Agropecuarias, Universidad de la Salle. Carrera. 7 No. 172-85. Bogotá, Colombia. (Received: November 13, 2014; accepted: January 27, 2016) doi: 10.17533/udea.rccp.v29n2a06 Summary Background: an optimal formulation for vitrifying in vitro-produced (IVP) bovine embryos is currently unavailable. Objective: to estimate whether differences in composition of vitrification solutions may affect the viability of IVP embryos as compared to that of embryos cryopreserved by a conventional slow-freezing method. Methods: bovine IVP embryos were cryopreserved by two methods: 1) a slow controlled-rate (1.5 M ethylene glycol (EG)), or 2) vitrification by using two different vitrification and thawing/warming solutions: (1) Protocol V1: commercial vitrification Kit, and (2) Protocol V2: defined vitrification (20% EG; 20% dimethyl sulfoxide (DMSO); 20% fetal bovine serum (FBS)) and warming (20% FBS; 0.2 M sucrose) solutions. Embryo viability was recorded at 24, 48, and 72 h after thawing/warming by evaluating the number of embryos that re-expanded and developed to the hatching blastocyst stage. Results: embryo survival rate was affected by the method of cryopreservation, where the frequency of embryos that re-expanded at 24 h after thawing/warming was higher for embryos vitrified with protocols V1 and V2 (89.0%, 86.2%, respectively) compared to those cryopreserved by the slow-controlled rate method (73.6%, p<0.05). Similarly, higher percentage of embryos cryopreserved by vitrification hatched at 72 h, where protocol V2 resulted in higher percentage of hatched embryos (84.3%) compared to protocol V1 (64,0%, p<0.05), and both were higher compared to the slowcontrolled rate method (55.2%, p<0.05). Conclusions: the method of cryopreservation and composition of the vitrification solution have a direct effect on the viability of bovine IVP embryos.

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“…Moreover it could open the door to large amounts of molecular data, principally when compared with their in vivo produced counterparts (Stinshoff et al, 2011). So, there is still a need to conduct studies that involve the most efficient vitrification elements from cryoprotectants to cryodevices.…”

Section: Introductionmentioning

confidence: 99%

The effect of vitrification of immature bovine oocytes to the subsequentin vitrodevelopment and gene expression

Faheem

Baron

Carvalhais

et al. 2014

Zygote

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Immature bovine oocytes were vitrified using the cryotop method and their post-warming survivability and capability to undergo in vitro maturation, fertilization and subsequent embryonic development were evaluated. In addition throughout the embryonic 2-cell, 4-cell, morula and blastocyst stages, the expression of four developmentally important genes (Cx43, CDH1, DNMT1 and HSPA14) was analysed using the real-time polymerase chain reaction (PCR). Immature oocytes (n = 550) were randomly assigned to non-vitrified (fresh) or cryotop vitrification groups using ethylene glycol (EG) with 1,2 propanediol (PROH) or dimethylsulphoxide (DMSO). After warming, oocytes survivability, embryo cleavage and embryonic developmental rates were not statistically different between the two cryoprotectants groups. However, the DMSO group had a lower (P < 0.05) oocyte maturation rate compared with the fresh and PROH groups. For morula and blastocyst rates, the DMSO group achieved a lower (P < 0.05) morula rate compared with the fresh group, while at the blastocyst stage, there were no differences between fresh and both cryoprotectants groups. For molecular analysis, at the 4-cell stage, most studied genes showed an inconsistent pattern of expression either from the PROH or DMSO groups. Noteworthily, these differences were limited at the morula and blastocyst stages. In conclusion, the cryotop method is sufficient for vitrification of immature bovine oocytes, both for embryonic developmental competence and at the molecular level. Moreover, PROH showed some advantage over DMSO as a cryoprotectant.

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Cryopreservation affects the quality of in vitro produced bovine embryos at the molecular level

Cited by 65 publications

References 45 publications

Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

Oocyte pre-IVM with caffeine improves bovine embryo survival after vitrification

Cryopreservation method and composition of the vitrification solution affect viability of in vitro bovine embryos

The effect of vitrification of immature bovine oocytes to the subsequentin vitrodevelopment and gene expression

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