Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione

Chatterjee, Suvro; Lamirande, Eve de; Gagnon, Claude

doi:10.1002/mrd.1115

Cited by 157 publications

(100 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This was contradictory to studies in humans (de Lamirande and Gagnon 1992), mice (Baiardi et al 1997), and horses (Baumber et al 2000) where deterioration of sperm motility was observed in the precence of free radicals. However, our results seemed lower than the findings of Chatterjee et al (2001), in that the addition of GGSG to the freezing extender reduced by 35% the loss of motility of spermatozoa undergoing a freezing-thawing cycle. These contradictory results may be due to the differences in extender composition and glutathione concentration.…”

Section: Discussioncontrasting

confidence: 96%

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Sarıözkan¹,

Tuncer²,

Bucak³

et al. 2009

Acta Vet. Brno

View full text Add to dashboard Cite

Cryopreservation is associated with the production of reactive oxygen substances (ROS), which lead to lipid peroxidation of sperm membranes, resulting in a loss of sperm motility, viability and fertility. The aim of this study was to determine effects of the antioxidants of oxidized glutathione (GSSG), reduced glutathione (GSH) and bovine serum albumin (BSA) on standard semen indicators (motility, acrosome and total abnormalities, HOST), endogenous antioxidant enzyme activities and fertilizing ability of frozen-thawed bull semen.Eighteen ejaculates from each of 3 Holstein bulls were collected using an artificial vagina and 9 replicates of the ejaculates were diluted with a Bioxcell ® -based extender supplemented with antioxidants, including BSA (5 mg/ml), GSH (2 mM), GGSG (2 mM), and an extender containing no antioxidants (control). Insemination doses (1.5 × 10 7 sperm/0.25 ml straw) were prepared for the insemination of cows at observed oestrus. Supplementation with antioxidants led to lower percentages of acrosome damage (4.0 ± 0.5%, 4.4 ± 0.5%, 4.0 ± 0.3%, respectively) and total abnormalities (10.3 ± 0.7%, 9.7 ± 0.8%, 10.4 ± 0.6%), compared to the controls (6.5 ± 0.6 and 14.9 ± 1.1% P < 0.01). Pregnancy rate after insemination was highest (72.2%) in the group which was given BSA (P < 0.05). There were no significant differences among groups in GSH and glutathione peroxidase (GSH-PX) enzyme activities. Superoxide dismutase (SOD) activities (0.05 ± 0.005, 0.03 ± 0.005, 0.05 ± 0.009 μkat/g protein, respectively) in all of the experimental groups with antioxidants were lower than the control group (0.11 ± 0.024 μkat/g protein, P < 0.001). Furthermore, BSA increased (P < 0.001) the activity of catalase (CAT, 304.23 ± 114.69 μkat/g protein), following the freezing-thawing process. Bull semen, freezing, artificial insemination, antioxidant activities, lipid peroxidationSemen cryopreservation has allowed specific opportunities for the conservation and widespread dissemination of valuable genetic resources through sperm banks, the guarantee of a constant commercial supply of semen, and collaboration in breed improvement programs by means of artificial insemination (AI) (Holt et al. 1997). Some of the problems encountered using AI in pigs, sheep and exotic species include decreased motility, membrane integrity and fertilizing potential of spermatozoa during cryopreservation, premature activation in the female genital tract, and altered membrane responses to physiological stimuli of sperm that survive the freezing-thawing process (Viswanath and Shannon 2000). It is well known that phospholipids in the sperm plasma membrane undergo peroxidation, which results in the formation of reactive oxygen substances (ROS, namely, superoxide anion radical, hydrogen peroxide) and lipid hydroperoxides (Alvarez and Storey 2005). Excessive generation of ROS by immature and abnormal spermatozoa, and sperm processing (e.g., extending, freezingthawing process), accompanied by low scavenging and antioxidant concentrations in semina...

show abstract

Section: Discussioncontrasting

confidence: 96%

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Sarıözkan¹,

Tuncer²,

Bucak³

et al. 2009

Acta Vet. Brno

View full text Add to dashboard Cite

show abstract

“…In addition, L-cysteine has the ability to pass into cells rapidly, and to be transformed into taurine, which, combined with a fatty acid in the plasma membrane, is transformed to acyl-taurine, which can improve surfactant properties and osmoregulation of the sperm membrane [24,28]. Furthermore, L-cysteine also has the ability to reduce lipid peroxidation that produces free radicals, and thus protect spermatozoa from ROS [2,4,5]. Besides being active as a glutathione precursor, L-cysteine can also account for a protective effect in the extracellular milieu, primarily due to its nucleophilic and antioxidant properties [30].…”

Section: Discussionmentioning

confidence: 99%

“…Weir and Robaire [3] reported that the seminal plasma consists of enzymatic antioxidants, i.e. glutathione reductase, glutathione peroxidases, superoxide dismutase and catalase, which have the ability to reduce lipid-peroxidation-producing free radicals and thus to protect spermatozoa from ROS [2,4,5]. During cryopreservation, it has been demonstrated that some steps of cryopreservation, such as discarding of seminal plasma by centrifugation and reduction in temperature, result in reduced quality and quantity of antioxidants and an increase in the quantity of ROS contained in the semen [6].…”

Section: Introductionmentioning

confidence: 99%

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Kaeoket¹,

Chanapiwat²,

Tummaruk³

et al. 2010

Asian J Androl

View full text Add to dashboard Cite

Cryopreservation is associated with the production of reactive oxygen species, which leads to lipid peroxidation of the sperm membrane and consequently a reduction in sperm motility and decreased fertility potential. The aim of this study was to determine the optimal concentration of L-cysteine needed for cryopreservation of boar semen. Twelve boars provided semen of proven motility and morphology for this study. The semen was divided into four portions in which the lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with various concentrations of L-cysteine to reach 0 mmol L −1 (group I, control), 5 mmol L −1 (group II), 10 mmol L −1 (group III) and 15 mmol L −1 (group IV). Semen suspensions were loaded in straws (0.5 mL) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability using SYBR-14/EthD-1 staining and acrosome integrity using FITC-PNA/EthD-1 staining. There was a significantly higher (P < 0.01) percentage of progressive motility, viability and acrosomal integrity in two L-cysteine-supplemented groups (group II and group III) compared with the control. There was a biphasic effect of L-cysteine, with the highest percentage of progressive motility, viability and acrosomal integrity in group III. In conclusion, 5 or 10 mmol L −1 was the optimum concentration of L-cysteine to be added to the LEY extender for improving the quality of frozen-thawed boar semen.

show abstract

“…Recently, it has also been suggested that gamma-oryzanol, a group of phytosterol ferulates, is responsible for the antioxidant activity in living cells via ROS scavenging and inhibition of ROS production [18]. In addition, gamma-oryzanol has the ability to prevent lipoperoxidation that produces free radicals (e.g., lipid peroxide, hydroperoxyl radical, hydroperoxide, alkoxyl radical) [31] and thus protect spermatozoa from ROS [2,11,32,47]. Furthermore, gamma-oryzanol may also account for a protective effect in the extracellular milieu, predominantly due to its antioxidant properties [22].…”

Section: Discussionmentioning

confidence: 99%

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

Kaeoket

Donto

Nualnoy

et al. 2012

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. The aim of this study was to determine the effect of gamma-oryzanol-enriched -rice bran oil on the quality of cryopreserved boar semen. Ten boars provided semen of proven motility and morphology for this study. The semen was divided into three portions in which lactose-egg yolk (LEY) extender used to resuspend the centrifuged sperm pellet was supplemented with 2 types of rice bran oils, at a gamma-oryzanol concentration of 0 mg/ml of lactose egg yolk (LEY) freezing extender (group A, control), 0.1 mg/ml(0.16 mMol) of freezing extender (group B) and 0.1 mg/ml of freezing extender (group C). Semen suspensions were loaded in medium straws (0.5 ml) and placed in a controlled-rate freezer. After cryopreservation, frozen semen samples were thawed and investigated for progressive motility, viability and acrosomal integrity. There was a significantly higher percentage of progressive motility (34 versus 47.0 and 48.5, P<0.001), viability (35.5 versus 48.1 and 50.1, P<0.001) and acrosomal integrity (39.8 versus 50.8 and 54.9, P<0.001) in the gamma-oryzanolenriched rice bran oil-supplemented groups (groups B, C) than in the control group (group C), respectively. In conclusion, addition of gamma-oryzanol-enriched rice bran oil to LEY freezing extender is appropriated for improving the quality of frozen-thawed boar semen. Cryopreservation of boar spermatozoa offers an effective way to maintain their valuable genetic material and transport it between countries, thereby minimizing boar transportation. It has been demonstrated that certain processes in cryopreservation including elimination of seminal plasma by centrifugation and reduction in the temperature result in cold shock, owing to a decrease in quantity of antioxidants and an increase in reactive oxygen species (ROS) contents in the semen [6,8,43]. The ROS can lead to oxidative stress and lipid peroxidation of the sperm plasma membrane, which have detrimental effects on spermatozoa during cryopreservation, for instance, reduction in progressive motility, acrosomal integrity, sperm-oocyte fusion and fertilizing potential [1-3, 17, 41, 53].During the past decade, several studies have focused on supplementation of freezing extender for frozen boar semen with a variety of antioxidants (e.g., vitamin E, vitamin C, L-cysteine, glutathione, taurine, pyruvate, SOD, catalase) in an attempt to minimize the detrimental effects of ROS, which occur during the freezing process [7,10,16,23,26,38,39].Gamma-oryzanol, a phytosteryl ferulate mixture extracted from rice bran oil, has received a great deal of attention, because of its various significant health-promoting functions such as antioxidant activity, inhibition of lipoperoxidation by its scavenging activity [4], reduction in LDL cholesterol and induction of HDL cholesterol [40,41,48] and inhibition of platelet aggregation [13]. In humans, many studies have been reported on its potential implications as a UV-A filter in sunscreen cosmetics [14] and as a treatment for type 2 diabetes mellitus [12] and allergic reactions [3...

show abstract

Cryopreservation alters membrane sulfhydryl status of bull spermatozoa: Protection by oxidized glutathione

Cited by 157 publications

References 31 publications

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Influence of Various Antioxidants on Microscopic-Oxidative Stress Indicators and Fertilizing Ability of Frozen-Thawed Bull Semen

Supplemental effect of varying L-cysteine concentrations on the quality of cryopreserved boar semen

Effect of Gamma-Oryzanol-Enriched Rice Bran Oil on Quality of Cryopreserved Boar Semen

Contact Info

Product

Resources

About