Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa

Pini, Taylor; Rickard, J.P.; Leahy, Tamara; Crossett, Ben; Druart, Xavier; Graaf, S.P. de

doi:10.1016/j.jprot.2018.04.001

Cited by 51 publications

(29 citation statements)

References 77 publications

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“…In consequence, the lipid-protein interactions required for a proper membrane activity are disturbed [9], and some sperm surface proteins as well as membrane proteins are lost or translocated with the consequent loss of their function. For example, proteins involved in capacitation, sperm-oocyte interaction and gamete fusion, such as TCP1, LOC101123268, RPN1, P25b, HEXB, CSNK1G2, ICA, LOC101123216, ADAM2 and TIMP-2, decreased in abundance in ram, gazelle and bull sperm after cryopreservation [38][39][40][41], while another protein associated with fertilization, HSP70, was lost in buffalo sperm [42]. Other proteins involved in transport, membrane stabilization and protection against lipid peroxidation or cold-shock, such as GLUT, CLU, BSP5, BSP1, aSFP, HSPA4L, TRAP1, GPX4 and GPX5 also decreased in abundance in these species along with antiapoptotic and decapacitating proteins (CSNK2A2 and Spermadhesin Z13) [38,40,43,44].…”

Section: Molecular Changes In the Sperm Plasma Membrane During Cryoprmentioning

confidence: 99%

“…Recently, a novel cryoprotective agent, carboxylated poly-L-lysine, has been used to reduce glycerol concentration in the freezing medium, enhancing in vivo fertility of cryopreserved buffalo and bull sperm [131,132]. Regarding non-permeating cryoprotectants, it has been reported that egg yolk also alters the proteome of ram sperm before cryopreservation [40]. Therefore, special attention should be payed to sperm-cryoprotectant interactions since these interactions may affect sperm cryopreservation outcomes.…”

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

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Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

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Sperm cryopreservation represents a powerful tool for livestock breeding. Several efforts have been made to improve the efficiency of sperm cryopreservation in different ruminant species. However, a significant amount of sperm still suffers considerable cryodamage, which may affect sperm quality and fertility. Recently, the use of different “omics” technologies in sperm cryobiology, especially proteomics studies, has led to a better understanding of the molecular modifications induced by sperm cryopreservation, facilitating the identification of different freezability biomarkers and certain proteins that can be added before cryopreservation to enhance sperm cryosurvival. This review provides an updated overview of the molecular mechanisms involved in sperm cryodamage, which are in part responsible for the structural, functional and fertility changes observed in frozen–thawed ruminant sperm. Moreover, the molecular basis of those factors that can affect the sperm freezing resilience of different ruminant species is also discussed as well as the molecular aspects of those novel strategies that have been developed to reduce sperm cryodamage, including new cryoprotectants, antioxidants, proteins, nanoparticles and vitrification.

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Section: Molecular Changes In the Sperm Plasma Membrane During Cryoprmentioning

confidence: 99%

Section: Molecular Aspects Of Those Novel Strategies To Reduce Sperm mentioning

confidence: 99%

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Peris-Frau¹,

Soler²,

Iniesta‐Cuerda³

et al. 2020

IJMS

147

118

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 92%

“…However, the best total and progressive motility values were obtained when samples were cryopreserved with EY and SP treatment was applied to Percoll ® selected sperm. These results might be explained by two reported evidences: (a) the loss of SP and EY proteins from the sperm surface after freeze‐thaw (Pini et al, ; Ramírez‐Vasquez et al, ) and (b) the interaction between SP proteins and EY lipoproteins (Bergeron et al, ; Manjunath et al, ). SP added on selected sperm after freeze‐thaw should restore the membrane ligands without either competing and/or being sequestered by egg yolk components, thus stabilizing membrane as supported by our PM integrity results.…”

Section: Discussionmentioning

confidence: 97%

“…Our group has been successful in improving ram sperm parameters by adding SP or their active components after thawing and selection, probably because of prompting sperm 'stabilizing molecules' direct interactions (Bernardini et al, 2011;Dominguez et al, 2008;Ledesma et al, 2016;Zalazar, Ledesma, Hozbor, & Cesari, 2016). Moreover, we and other researchers have recently demonstrated that sperm surface is modified by extender components (Pini et al, 2018;.…”

Section: Discussionmentioning

confidence: 97%

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Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Ramírez-Vasquez

Cesari

Greco

et al. 2019

Reprod Domestic Animals

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Seminal plasma (SP) proteins interact with sperm plasma membrane (PM) modulating its functionality. It has been shown that SP proteins can reverse the damage caused by freeze‐thaw; however in these studies, SP has been added to washed sperm (i.e., cells depleted from homologous SP and extender). The aim of the current study was to assess whether the egg yolk‐based extender (EY) modifies SP ability to ameliorate sperm parameters in frozen‐thawed ram spermatozoa. Ejaculates were diluted in EY or soybean lecithin‐based extender (SL) and evaluated before and after freezing to measure the cell damage according to the extender. Even when all classical parameters decreased after freezing, as expected (p < .05), there was no effect of the extender. SP treatment was applied after freeze‐thaw. Sperm were incubated with SP (20% v/v) in the presence of either EY or SL, and sperm parameters were assessed after thawing compared with the same treatments after Percoll sperm selection (washed). Treatments with 20% SP improved sperm total and progressive motility compared with controls regardless of washing and extender (p < .05); however, washed sperm showed higher percentage of total sperm motility compared with those unwashed (p < .05). Moreover, treatment with 20% SP showed significantly higher percentages of PM integrity, sperm with intact acrosomes, integrity of chromatin and non‐capacitated sperm in samples diluted with EY when washed before treatment compared with the other conditions (p < .05). It was concluded that the presence of the extenders and particularly egg yolk alters the SP capacity to reduce the cryodamage.

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Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality

et al. 2020

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Cryopreservation and egg yolk medium alter the proteome of ram spermatozoa

Cited by 51 publications

References 77 publications

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Sperm Cryodamage in Ruminants: Understanding the Molecular Changes Induced by the Cryopreservation Process to Optimize Sperm Quality

Extenders modify the seminal plasma ability to minimize freeze‐thaw damage on ram sperm

Elucidating the processes and pathways enriched in buffalo sperm proteome in regulating semen quality

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