Cryopreservation and genetic stability of Dendranthema grandiflora Tzvelev in vitro cultures

Martı́n, Carmen; González‐Benito, M. Elena

doi:10.2137/145960609789267560

Cited by 6 publications

(2 citation statements)

References 21 publications

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“…In the past it was not possible to completely maintain the stability of chrysanthemum stored in LN, either by using classical/two-step freezing (Fukai and Oe 1990;Fukai et al 1994), as well as by modern vitrification-based techniques (Martín and González-Benito 2009;Martín et al 2011). Our study showed that the encapsulation-dehydration technique based on 14-day preculture with 0.09 M sucrose and 10 µM ABA, followed by 4-day osmotic dehydration and 3-hour desiccation is optimal for the shoot tip cryopreservation of three chrysanthemum cultivars.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Kulus

Rewers

Serocka

et al. 2019

Plant Cell Tiss Organ Cult

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Little is known about the stability of plant chimeras after exposure to liquid nitrogen. The aim of this study was to evaluate the effect of cryopreservation on the cytogenetic and genetic stability, as well as vegetative and generative growth of chrysanthemums that are a solid mutant ('Richmond') or periclinal chimeras ('Lady Orange' and 'Lady Salmon'). The following encapsulation-dehydration protocol of shoot tip cryopreservation was applied: 0.09 M sucrose concentration and 10 µM abscisic acid during a two-week preculture, followed by a 4-day osmotic dehydration and 3-hour desiccation. Both, the cryopreservation-recovered and control plants were characterized by the same relative DNA content and chromosome number (ploidy level). By applying RAPD and ISSR markers, 18 and 1 and 0 polymorphic loci within cryopreservation-derived 'Lady Orange' and 'Lady Salmon' and 'Richmond' were detected, respectively. The recovered after cryopreservation and control plants had the same colour, diameter and fresh weight of inflorescences, as well as length of ray florets. Cryopreservation also did not affect the flowering time in chrysanthemum. The biochemical assay did not reveal any alternations in the level of anthocyanins and carotenoids in flowers. It was noted, however, that some of the leaves of the cryopreservation-recovered plants were shorter and/or narrower. They had a reduced chlorophyll content, and their internodes were shorter when compared to the untreated control. The inflorescences of 'Lady Salmon' opened slower, but faded faster. In conclusion, these results illustrate the practicability of a cryopreservation method that completely protects the chimera chrysanthemum identity. Key messageCryopreservation via encapsulation-dehydration technique affects the vegetative growth of chrysanthemum during the first cultivation season but does not disturb its chimeric structure.

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Section: Discussionmentioning

confidence: 99%

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Kulus

Rewers

Serocka

et al. 2019

Plant Cell Tiss Organ Cult

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“…The presence of the bead protects the explants from the toxic influence of concentrated sucrose solutions and allows for their desiccation to low moisture content (Engelmann 2008). However, previous studies with chrysanthemum encapsulation-dehydration have been rather unsatisfactory, resulting in low survival (20-45%) or poor regrowth rates (12%) and regeneration of multiple shoots through callus (Halmagyi et al 2004, Martin and Gonzalez-Benito 2009.…”

Section: Introductionmentioning

confidence: 99%

Cryopreservation of in vitro-grown shoot tips of chrysanthemum by encapsulation-dehydration

Zalewska

Kulus

2013

Folia Horticulturae

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Chrysanthemums are amongst the most economically important flowers in the world. The protection and storage of these valuable genetic resources is of great importance. Today, cryopreservation, or the storage of biological material at the temperature of liquid nitrogen (-196°C), is believed to be the most promising long-term storage method. To optimise the cryopreservation protocol, the shoot tips of Chrysanthemum × grandiflorum /Ramat./ Kitam. 'Lady Orange' and 'Lady Salmon' mutants were cryopreserved using the encapsulation-dehydration technique. During the experiment, the influence of sucrose concentration (2, 3 and 6%) during preculture and the concentration of kinetin (0.25, 0.5, 0.75 and 1.0 mg dm -3 ) in the regrowth medium were tested. A higher survival rate was observed for 'Lady Salmon'. In general, the media with higher sucrose levels provided the best survival and recovery rates (35-40%). Kinetin had no influence on the survival rate; however, it influenced the morphogenesis of the plants. The lowest number of explants forming multiple shoots was observed on the medium with the lowest sucrose (during preculture) and kinetin (in the recovery medium) concentration. On the other hand, the best rhizogenesis efficiency was observed when 0.25 mg dm -3 kinetin was added. In conclusion, the composition of both preculture and recovery media need to be adjusted to single cultivars. The use of 3% sucrose (preculture) and 0.25 mg dm -3 kinetin (recovery) seems reasonable, since it guarantees a satisfying recovery rate of the explants and at the same time prevents the formation of callus and multiple shoots, stimulating the rooting instead.

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Plant Cryopreservation for Biotechnology and Breeding

Попова

Shukla

Kim

et al. 2015

Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools

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Cryopreservation and genetic stability of Dendranthema grandiflora Tzvelev in vitro cultures

Cited by 6 publications

References 21 publications

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Cryopreservation by encapsulation-dehydration affects the vegetative growth of chrysanthemum but does not disturb its chimeric structure

Cryopreservation of in vitro-grown shoot tips of chrysanthemum by encapsulation-dehydration

Plant Cryopreservation for Biotechnology and Breeding

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