Cryopreservation and germinative behavior of Passiflora spp. seeds

Silva, Jailton de Jesus; Junghans, T. G.; Lêdo, Carlos Alberto da Silva; Silva, Fabiane de Lima; Souza, Everton Hilo de; Hongyu, Kuang; Souza, Fernanda Vidigal Duarte

doi:10.1007/s13205-022-03329-6

Cited by 3 publications

(1 citation statement)

References 41 publications

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“…However, only P. foetida shoot tips and seeds had a post-freeze recovery of 60% when cryopreserved via the encapsulation-vitrification technique [ 104 – 106 ]. Seed cryopreservation of 10 Passiflora species in LN showed that the final germination percentage was not affected in LN [ 105 , 107 ].…”

Section: Cryopreservation Of Passiflora Speciesmentioning

confidence: 99%

Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement

et al. 2023

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Passion fruit is an essential commercial plant in the tropics and subtropics, which has lately seen a rise in demand for high-quality fruits and large-scale production. Generally, different species of passion fruit (Passiflora sp.) are propagated by sexual reproduction. However, asexual reproduction, such as stem cuttings, grafting, or tissue culture, is also available and advantageous in many instances. Recent research on passion fruit has concentrated on improving and establishing methodologies for embryogenesis, clonal proliferation via (somatic embryos), homozygote regeneration (by anther culture), germplasm preservation (via cryopreservation), and genetic transformation. These developments have resulted in potentially new directions for asexual propagation. Even though effective embryo culture and cryogenics are now available, however the limited frequency of embryogenic callus transformation to ex-vitro seedlings still restricts the substantial clonal replication of passion fruit. Here, in this review the advancement related to biotechnological approaches and the current understanding of Passiflora tissue culture. In vitro culture, organogenesis, cryopreservation, breeding, and productivity of Passiflora will significantly improve with novel propagation approaches, which could be applied to a wider range of germplasm.

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Section: Cryopreservation Of Passiflora Speciesmentioning

confidence: 99%

Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement

et al. 2023

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Seed morphoanatomy of the genus PassifloraL. (Passifloraceae) by scanning electron microscopy and light microscopy

Silva

Souza

Junghans

et al. 2022

Microscopy Res & Technique

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Morphoanatomical analysis of seeds contributes to knowledge of the development of seedlings and identification of species, as well as supporting conservation studies. The conservation of the species belonging to the Passiflora genus is crucial due to of the threats to the genetic resources of these species. Thus, the objective of this study was to morphoanatomically characterize Passiflora seeds, verify possible injuries to the tissues after cryopreservation and thus contribute to the conservation strategies of the species of this genus. Initially, seeds of Passiflora coccinea, P. edulis, P. gibertii, P. maliformis, P. morifolia, P. setacea, P. suberosa, and P. tenuifila collected from the Passion Fruit Active Germplasm Bank of the Embrapa Cassava and Fruits research unit (Embrapa Mandioca e Fruticultura) were analyzed. Then, their length, width and thickness, shape of the base and tip, and ornamentations present on the body and edge of the seeds were evaluated. The seeds of the species were placed in cryotubes and immersed in liquid nitrogen to assess possible cryoinjuries. The tegument and tissues of the seeds were examined by scanning electron microscopy. The seeds had varied biometric data, with average values of 4.63 mm for length, 3.28 mm for width, and 1.51 mm for thickness. Six ornamentation types were observed: reticulate for the species P. coccinea; finely reticulate for P. edulis; foveolate reticulate for P. gibertii and P. setacea; alveolate reticulate for P. maliformis and P. tenuifila; coarsely reticulate for P. morifolia; and falsifoveolate reticulate for P. suberosa. Some seeds suffered tegument cracks due to the freezing in liquid nitrogen, but without physiological damages to the embryo and endosperm. The cryopreservation of the seeds in the presence of the tegument significantly reduced the cryoinjuries caused to the embryo. Cryopreservation can be promising for long-term conservation of passion fruit seeds.

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Salt tolerance strategy in passion fruit genotypes during germination and seedling growth and spectrophotometric quantification of hydrogen peroxide (H2O2)

Santos,

Jesus,

Sampaio

et al. 2024

Scientia Horticulturae

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Cryopreservation and germinative behavior of Passiflora spp. seeds

Cited by 3 publications

References 41 publications

Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement

Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement

Seed morphoanatomy of the genus PassifloraL. (Passifloraceae) by scanning electron microscopy and light microscopy

Salt tolerance strategy in passion fruit genotypes during germination and seedling growth and spectrophotometric quantification of hydrogen peroxide (H2O2)

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