Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

Magnotti, Caio; Cerqueira, Vinícius Ronzani; Lee‐Estévez, Manuel; Farías, Jorge G.; Valdebenito, Iván; Figueroa, Elías

doi:10.1111/raq.12145

Cited by 90 publications

(63 citation statements)

References 84 publications

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“…One of the ways to do so is through germplasm cryopreservation. Perhaps not surprisingly, aside from research in humans, studies on germplasm cryopreservation have largely focused on advances in technology for livestock (e.g., Morrell & Mayer, 2017;Sieme & Oldenhof, 2015;Singh, Mal, & Singla, 2017) and aquaculture management (e.g., reviews by Asturiano, Cabrita, & Horváth, 2017;Martinez-Paramo et al, 2017;Magnotti et al, 2018). Perhaps not surprisingly, aside from research in humans, studies on germplasm cryopreservation have largely focused on advances in technology for livestock (e.g., Morrell & Mayer, 2017;Sieme & Oldenhof, 2015;Singh, Mal, & Singla, 2017) and aquaculture management (e.g., reviews by Asturiano, Cabrita, & Horváth, 2017;Martinez-Paramo et al, 2017;Magnotti et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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Novel conservation methods have become increasingly important in protecting our remaining biodiversity in the face of unprecedented rates of species declines. One method of maintaining genetic and species diversity is through germplasm cryopreservation. However, our knowledge of cryopreservation relies heavily on studies in domestic or aquaculture species. Moreover, most studies are narrowly focused on a single species. Consequently, the broader application of these methods to wildlife conservation is often unknown or assumed. Here, we examine the response of four anuran species from two families (Anaxyrus fowleri, Anaxyrus baxteri, Lithobates pipiens, and Lithobates sevosus) to three sperm cryopreservation treatments (5, 10, and 15% N,N-dimethylformamide with 10% trehalose). Within each family, we selected a congeneric species pair with one common, nonthreatened species and one endangered species. We found that endangered species had significantly lower initial sperm quality, though recovery rate of sperm after cryopreservation was not significantly different between nonthreatened and endangered species. Overall, responses to the three treatments were consistent across species, with 5% DMFA with 10% trehalose producing the highest recovery rates in all four species. In addition, cryopreserved sperm were used to successfully produce hatchlings in both of the nonthreatened species. Our results demonstrate that standardized methods developed for anurans can be applied more widely across families and can be transferred from model species to species of conservation concern.These findings form a basis for further exploration into cryopreservation as an effective tool for wildlife conservation in amphibians. K E Y W O R D Samphibian, assisted reproductive technology, Bufonidae, cryobiology, Ranidae, sperm motility

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Section: Introductionmentioning

confidence: 99%

Applying cryopreservation to anuran conservation biology

Poo

Hinkson

2019

Conservat Sci and Prac

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“…; Magnotti et al . ). It is also possible, in some cases, that DNA defects in the sperm may be repaired by the oocyte repair system after fertilization, as in the case of Logurnus fossilis (Kopeika et al .…”

Section: Embryo Survival and Qualitymentioning

confidence: 97%

“…; Magnotti et al . ). On the other hand, several works have demonstrated that sperm cryopreservation generates damage to DNA in mammals (Hu et al .…”

Section: Introductionmentioning

confidence: 97%

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

Figueroa

Lee‐Estévez

Valdebenito

et al. 2018

Reviews in Aquaculture

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Sperm DNA quality and gene expression are crucial for early embryo development. Abnormal genomic processes can cause irreversible damage to totipotent cells, thereby altering the capacity for cell differentiation. Cryopreservation is a complex procedure that exposes cells to extreme conditions; it is therefore important to determine whether cryopreservation affects genomic stability. Despite this, few studies have focused on the effects of cryopreservation on the genomic stability of fish sperm. Thus, information is lacking to determine how the cryopreservation of fish sperm affects embryo quality and subsequently aquaculture. The aim of this review was to compile background information pertaining to the study of genomic stability in cryopreserved sperm. In this way, we hope to characterize more clearly the embryonic development of fish that are of biological and economic interest. We also discuss the potential use of antioxidants in cryopreservation to help preserve DNA integrity, gene expression and embryo quality. There is a current need to evaluate the use of potential biomarkers of genomic integrity to establish the effects of cryopreservation on the DNA quality of fish gametes and embryos.

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“…However, due to its simplicity, speed, and utility for field applications with no equipment required, it offers a new approach to preserve genetic resources and to reconstitute strains or lines (Moore and Bonilla 2006; Saragusty and Arav 2011; Magnotti et al, in press). Vitrification is best suited for use in three areas: biomedical research fish models, genetically improved lines, and imperiled species.…”

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confidence: 99%

“…Most recently, sperm motility and sperm head morphometry were evaluated after vitrification of the marine European Eel Anguilla anguilla (Kasa et al, in press). However, these studies did not attempt fertilization or offspring production (Magnotti et al, in press). …”

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confidence: 99%

Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes

Cuevas‐Uribe

Hu²,

Daniels

et al. 2017

N American J Aquac

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The Southern Flounder Paralichthys lethostigma is a high-value species and a promising aquaculture candidate. Because sperm volume can be limited in this species (<500 μL), new sperm cryopreservation methods need to be evaluated. Vitrification is an alternative to conventional slow-rate freezing, whereby small volumes are cryopreserved at high cooling rates (>1,000°C/min). The goal of this work was to develop a standardized approach for vitrification of Southern Flounder sperm. The specific objectives were to (1) evaluate thawing methods and vitrification solutions, (2) evaluate the postthaw membrane integrity of sperm vitrified in different cryoprotectant solutions, (3) examine the relationship between membrane integrity and motility, and (4) evaluate the ability of vitrified sperm to fertilize eggs. From the vitrification solutions tested, the highest postthaw motility (28 ± 9% [mean ± SD]) and membrane integrity (11 ± 4%) was observed for 20% ethylene glycol plus 20% glycerol. There was no significant difference in postthaw motility of sperm thawed at 21°C or at 37°C. Fertilization from vitrified sperm in one trial yielded the same fertilization rate (50 ± 20%) as the fresh sperm control, while the sperm from the other two males yielded 3%. This is the first report of fertilization by vitrified sperm in a marine fish. Vitrification can be simple, fast, inexpensive, performed in the field, and, at least for small fishes, offers an alternative to conventional cryopreservation. Because of the minute volumes needed for ultrarapid cooling, vitrification is not presently suited as a production method for large fishes. Vitrification can be used to reconstitute lines from valuable culture species and biomedical models, conserve mutants for development of novel lines for ornamental aquaculture, and transport frozen sperm from the field to the repository to expand genetic resources.

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Cryopreservation and vitrification of fish semen: a review with special emphasis on marine species

Cited by 90 publications

References 84 publications

Applying cryopreservation to anuran conservation biology

Applying cryopreservation to anuran conservation biology

Potential biomarkers of DNA quality in cryopreserved fish sperm: impact on gene expression and embryonic development

Vitrification as an Alternative Approach for Sperm Cryopreservation in Marine Fishes

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