Cryopréservation d'isolats detoxoplasma gondiien culture cellulaire

Abstract

Summary : Toxoplasma go n d ii: F reeze-preservation in cell CULTUREFor a better preservation and identification of Toxoplasma gondii isolates, w e propose a new method of freezing of toxoplasma grown in THP-1 cell culture. A cystogenic strain isolated from foetal blood has been grown in these cells and frozen in liquid nitrogen. After thawing, toxoplasma recover the same growth rate and morphology in vitro and the same capacity to form brain cysts into mice compared to the initial strains. The freezing of the cell suspension provides a simple and appropriate method for preservation of Toxoplasma gondii within in « bank » isolates.