Cryopreservation in liquid nitrogen of cultured navel organe (Citrus sinensis Osb.) nucellar cells and subsequent plant regeneration

Kobayashi, Shozo; Sakai, Akira; Oiyama, I.

doi:10.1007/bf00116084

Cited by 27 publications

(8 citation statements)

References 15 publications

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“…Many publications indicate that characteristics such as growth rate, regeneration capability, ploidy levels and RAPD markers are retained after cryogenic storage (Kobayashi et al 1990;Wang et al 1994;Ward et al 1993). Solid regeneration medium is usually used for regrowth of cryopreserved explants (Hirai and Sakki 1999); nevertheless, some results suggest that liquid medium may be better (Pains et al 2002).…”

Section: In Vitro Conservation Of Date Palmmentioning

confidence: 97%

In Vitro Conservation of Date Palm Germplasm

Bekheet

2011

Date Palm Biotechnology

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Date palm (Phoenix dactylifera L.) germplasm is difficult to conserve and store in the form of offshoots or in field collections. Tissue culture technologies have had a major impact on the ex situ conservation of plant genetic resources. In vitro culture techniques supplement date palm conservation efforts and have been applied to germplasm collection, preservation and rapid clonal multiplication. In vitro storage methods have been developed for preservation of date palm germplasm and can be used efficiently for international exchange of germplasm because of their obvious advantages over in vivo material. Preservation of plant cells, meristems and somatic embryos has become an important tool for long-term storage of germplasm utilizing minimum space and low maintenance. Short-and mid-term storage is achieved by controlling environmental growth conditions and nutrient media composition. Long-term storage has been reported for in vitro cryopreservation of date palm cultures. Encapsulation of plant material in alginate beads has been suggested recently as a possible means of date palm germplasm exchange. Knowledge about germplasm diversity and genetic relationships are highly valuable tools in plant conservation strategies. In this regard, a number of molecular biology methods are currently available for analysis of genetic diversity in date palm genotypes. This chapter discusses the general issues and different aspects of plant biotechnology used for management and conservation of date palm cultivars.

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Section: In Vitro Conservation Of Date Palmmentioning

confidence: 97%

In Vitro Conservation of Date Palm Germplasm

Bekheet

2011

Date Palm Biotechnology

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“…Vasil (1983) stated that the genetic stability found in plants regenerated from callus or tissues of many species was considered to be the result of regeneration through somatic embryogenesis. Because of this stability, embryogenic callus cultures have been widely used for cryopreservation (Kartha et al, 1988;Uragami et al, 1989;Kobayashi et al, 1990). The genotype of white clover used in the present study has also genetic stability and high plant regeneration potential.…”

Section: Discussionmentioning

confidence: 93%

Plant regeneration of meristematic callus of white clover (Trifolium repens L.) cooled to −196°C by vitrification

Yamada

Sakai²,

Matsumura

et al. 1993

Euphytica

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A callus line of white clover capable of forming numerous meristemoids (meristematic cell masses) has been selected and subeultured on agar B5 medium containing 0.5 mg/1 2,4-D and 0.5 mg/1 kinetin for three years. The meristematic callus was successfully cryopreserved by vitrification and subsequently regenerated plants. Preculturing callus in liquid B5 medium containing 0.6 M sorbitol at 25 ° C for 16 hr was essential to the process. Precultured samples (50 mg) were transferred to a 1.8 ml plastic cryotube and then 1 ml of a highly concentrated cryoprotective solution (designated PVS2) was added and mixed. After treatment with PVS2 at 25 ° C for 7 min or 0 ° C for 20 min, the sample was directly plunged into LN. After rapid warming, PVS2 was drained from the cryotubes and replaced twice with liquid B5 medium containing 1.2 M sucrose. Samples were transferred onto filter disc over agar B5 medium. Some surviving cells in the cryopreserved meristematic callus proliferated and produced new meristemoids. After 30 days the meristematic callus was transferred onto hormone-free MS agar medium. The meristemoids developed directly into shoots and spontaneously formed roots. Plant regeneration efficiency expressed as a percent of control amounted to about 90%. This vitrification method appears promising as a routine method for cryopreserving meristematic callus of white clover.

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“…Embryogenic callus cultures have been demonstrated to survive repeated cryopreservation treatments with routinely used dimethylsulfoxide (DMSO) (Kobayashi et al, 1990;Aguilar et al, 1993). Cryopreserved cultures of various tissues including recalcitrant seeds, ovules, embryos, callus, etc can successfully be used in future after months of storage at low temperature.…”

Section: Discussionmentioning

confidence: 99%

Citrus biotechnology: Achievements, limitations and future directions

Singh

Rajam

2009

Physiol Mol Biol Plants

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Citrus is one of the most important commercial and nutritional fruit crops in the world, hence it needs to be improved to cater to the diverse needs of consumers and crop breeders. Genetic manipulation through conventional techniques in this genus is invariably a difficult task for plant breeders as it poses various biological limitations comprising long juvenile period, high heterozygosity, sexual incompatibility, nucellar polyembryony and large plant size that greatly hinder cultivar improvement. Hence, several attempts were made to improve Citrus sps. by using various in vitro techniques. Citrus sps are widely known for their recalcitrance to transformation and subsequent rooting, but constant research has led to the establishment of improved protocols to ensure the production of uniformly transformed plants, albeit with relatively low efficiency, depending upon the genotype. Genetic modification through Agrobacterium-mediated transformation has emerged as an important tool for introducing agronomically important genes into Citrus sps. Somatic hybridization has been applied to overcome self and cross-incompatibility barriers and generated inter-specific and inter-generic hybrids. Encouraging results have been achieved through transgenics for resistance against viruses and bacteria, thereby augmenting the yield and quality of the fruit. Now, when major transformation and regeneration protocols have sufficiently been standardized for important cultivars, ongoing citrus research focuses mainly on incorporating such genes in citrus genotypes that can combat different biotic and abiotic stresses. This review summarizes the advances made so far in Citrus biotechnology, and suggests some future directions of research in this fruit crop.

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Cryopreservation in liquid nitrogen of cultured navel organe (Citrus sinensis Osb.) nucellar cells and subsequent plant regeneration

Cited by 27 publications

References 15 publications

In Vitro Conservation of Date Palm Germplasm

In Vitro Conservation of Date Palm Germplasm

Plant regeneration of meristematic callus of white clover (Trifolium repens L.) cooled to −196°C by vitrification

Citrus biotechnology: Achievements, limitations and future directions

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