Cryopreservation of adult unrelated donor products in hematopoietic cell transplantation: the OneMatch experience and systematic review of the literature

Abstract

Future studies with standard outcome measures are needed to clarify the impact of cryopreservation on engraftment and other transplant outcomes. International guidelines that consider the ethical framework surrounding requests for donor product cryopreservation are needed.

Demand and usage of unrelated donor products for allogeneic haematopoietic cell transplantation during the COVID‐19 pandemic: A Canadian Blood Services Stem Cell Registry analysis

“…In response to the recommendations from national and international bodies, the majority of products collected from unrelated donors have been cryopreserved (68% of products collected from Canadian donors from March 2020 to June 2021) before infusion in comparison to 4% in the 12 months before the pandemic and approximately 7% noted in a historical cohort that was previously described [ 11 ]. With regard to adverse events related to cryopreservation, none of eight adverse event reports that were filed by CBS to the World Marrow Donor Association between January 2019 and March 2020 was related to cryopreservation, whereas between March 2020 and June 2021, a total of four adverse events were reported, all of which described impacts related to cryopreservation.…”

How do I structure logistic processes in preparation for outsourcing of cellular therapy manufacturing?

“…Noncultured HPCs and MNCs withstand cryopreservation relatively well. [21][22][23][24][25][26] However, cultured or gene manipulated cells and certain cell subtypes such as monocytes and NK cells are less resilient and experience phenotypic alterations postthaw. [27][28][29][30] Some loss of cell viability and function is inevitable with cryopreservation.…”

“…Noncultured HPCs and MNCs withstand cryopreservation relatively well . However, cultured or gene manipulated cells and certain cell subtypes such as monocytes and NK cells are less resilient and experience phenotypic alterations postthaw .…”

Effect of Cryopreservation on Autologous Chimeric Antigen Receptor T Cell Characteristics

“…The autologous PBMNC starting material is also often cryopreserved to allow for flexibility in scheduling manufacturing. 6 While hematopoietic stem cells survive cryopreservation and thawing well, and cryopreserved hematopoietic stem cell transplantation has been performed for many years, 7,8 the effects of the cryopreservation on cultured T cells is less certain. The data available suggest that the overall recovery of cryo-thawed T cells is marginally worse than that of hematopoietic stem cells.…”