Cryopreservation of Blood

Bohoněk, Miloš

doi:10.5772/36146

Cited by 7 publications

(7 citation statements)

References 20 publications

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“…In this study, we used a lower percentage of glycerol (10%) compared to that commonly used in the clinic (20–40%) to avoid aggressive cell wash during glycerol removal [ 8 ]. Nevertheless, it was found that 10% glycerol showed similar cryoprotective effect to the FDA-approved concentration of 20%-40% (data not shown).…”

Section: Discussionmentioning

confidence: 99%

“…There is wide range of CPAs used for RBCs cryopreservation but the only compound that has been clinically licensed for this purpose is glycerol. Glycerol is a cell-permeable compound and is used at a 1–2 M concentration for blood cell cryopreservation (20%—40% ratio, glycerol—blood) [ 8 ]. Cryopreservation of RBCs in glycerol is achieved using either high concentrations of glycerol and slow freezing at -80°C or low glycerol concentrations and fast freezing in liquid nitrogen.…”

Section: Introductionmentioning

confidence: 99%

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Salidroside as a Novel Protective Agent to Improve Red Blood Cell Cryopreservation

2016

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Glycerol and trehalose have been widely examined as protective agents in the cryopreservation of red blood cells (RBCs). However, the effectiveness of these reagents alone on cell viability is moderate. Here, the addition of salidroside attenuated oxidative damage of sheep RBCs prior to and post cryostorage. The supplementation of salidroside to the cryopreservation media containing 10% glycerol improved RBC survival by approximately 61.1±4.8% vs 37.9±4.6%. A smaller effect was seen in RBCs cryopreserved in 300 mM trehalose where the addition of salidroside improved survival by 7.6±0.3%. Furthermore, the addition of salidroside to cold storage solution demonstrated a significant reduction of haemolysis after 4 days for RBCs loaded with either glycerol or trehalose, compared to cells incubated without salidroside. RBCs survival was 2-fold greater following freezing in trehalose, compared with glycerol. After 10 days, salidroside enabled a lower haemolysis of 16.7±1.3% compared to 29.0±8.4% for cells incubated without salidroside. However, salidroside had no effect on RBCs which had been frozen in glycerol as the resulting haemolysis rate by day 10 was approximately 60%. Salidroside increased glutathione reductase activity and decreased lactate dehydrogenase activity. Furthermore, it led to reduced carbonylation of proteins in both glycerol and trehalose loaded cells. Finally, no effect on lipid peroxidation was found in the glycerol loaded RBCs although this was reduced in RBCs loaded with trehalose and salidroside. The present findings confirm the potential use of salidroside as a novel protective agent in cryopreservation and refrigerated storage of sheep RBCs.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Salidroside as a Novel Protective Agent to Improve Red Blood Cell Cryopreservation

2016

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“…The AS-3 solution has been validated for a shelf life of 14 days and was used in all other studies (8,9,11). To our knowledge, only the SAG-M additive solution, which is validated for 7 days, is available in Turkey.…”

Section: Discussionmentioning

confidence: 99%

“…The storage of frozen ECs has become an essential part of national blood crisis policies in several countries, as frozen ECs can be thawed and transfused rapidly in emergency situations without the need for laboratory tests. ECs are also cryopreserved for patients with rare blood types and are autologous blood for patients with red blood cell alloantibodies; clinical conditions that require cytomegalovirus (-) blood can also be considered in this context (11).…”

Section: Introductionmentioning

confidence: 99%

“…Although fresh ECs can be stored for up to 42 days by introducing additive solutions, they can be preserved for an extended time period by freezing in an efficient and reliable manner (11). Thus, cryopreservation of expired ECs, instead of disposal, and their use after thawing can not only prevent the inappropriate waste of resources but will also allow the use of ECs in times of need.…”

Section: Introductionmentioning

confidence: 99%

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In vitro efficacy of frozen erythrocytes: implementation of new strategicblood stores to alleviate resource shortage (issue revisited)

Çeti̇nkaya¹,

Eker²,

Ünlü³

et al. 2015

Turk J Med Sci

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Background/aim: Currently, the provision of blood products largely depends on walking blood banks and limited amounts of stored blood with short shelf lives. We aimed to compare the efficacy of erythrocyte concentrate (ECs) by pre-and postfreezing in vitro tests. Materials and methods:In our study, 10 ECs were glycerolized, frozen, thawed, and then deglycerolized using the Naval Blood Research Laboratory method. In addition to using the standard tests, ATP and 2,3-DPG levels and the viability of erythrocytes were also determined. Results:The prefreezing mean viability rates of erythrocytes changed from 89.7 ± 13.7% to 98.6 ± 1.8% after thawing and deglycerolization. Prefreezing and day 0 ATP levels (1.64 ± 0.15 µmol/g Hb and 1.81 ± 0.14 µmol/g Hb, respectively) were similar. The 2,3-DPG levels decreased from 18.09 ± 4.78 µmol/g Hb measured before the procedure to 10.41 ± 4.58 µmol/g Hb on day 0. The mean hemolysis rates and supernatant Hb levels changed from 0.21 ± 0.11% to 0.36 ± 0.12% and 1 ± 0.5 g/L to 1.5 ± 0.5 g/L, respectively. Conclusion:The test results showed the efficacy of the frozen-thawed ECs to be used in humans for a broad spectrum of clinical indications. As a part of a contingency plan, national frozen blood reserves need to be established.

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Ice Recrystallization Inhibition Is Insufficient to Explain Cryopreservation Abilities of Antifreeze Proteins

et al. 2022

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Antifreeze proteins (AFPs) and glycoproteins (AFGPs) are exemplary at modifying ice crystal growth and at inhibiting ice recrystallization (IRI) in frozen solutions. These properties make them highly attractive for cold storage and cryopreservation applications of biological tissue, food, and other water-based materials. The specific requirements for optimal cryostorage remain unknown, but high IRI activity has been proposed to be crucial. Here, we show that high IRI activity alone is insufficient to explain the beneficial effects of AF( G )Ps on human red blood cell (hRBC) survival. We show that AF( G )Ps with different IRI activities cause similar cell recoveries of hRBCs and that a modified AFGP variant with decreased IRI activity shows increased cell recovery. The AFGP variant was found to have enhanced interactions with a hRBC model membrane, indicating that the capability to stabilize cell membranes is another important factor for increasing the survival of cells after cryostorage. This information should be considered when designing novel synthetic cryoprotectants.

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Cryopreservation of Blood

Cited by 7 publications

References 20 publications

Salidroside as a Novel Protective Agent to Improve Red Blood Cell Cryopreservation

Salidroside as a Novel Protective Agent to Improve Red Blood Cell Cryopreservation

In vitro efficacy of frozen erythrocytes: implementation of new strategicblood stores to alleviate resource shortage (issue revisited)

Ice Recrystallization Inhibition Is Insufficient to Explain Cryopreservation Abilities of Antifreeze Proteins

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