Cryopreservation of carp sperm

Kurokura, Hisashi; Hirano, Reijiro; Tomita, Masakatsu; Iwahashi, Masao

doi:10.1016/0044-8486(84)90159-5

Cited by 140 publications

(82 citation statements)

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“…This may be due to the fact that V2E extender had proper concentration of ions and egg yolk. Earlier, the suitability of V2E extender for cryopreservation of sperm has been demonstrated in a number of fresh and marine water fishes including carp Labeo rohita, Cyprinus carpio and mullet Liza parsia (Kurokura et al, 1984;Gopalakrishnan et al, 2000;Sukumaran et al, 2003). Dilution of milt in an extender is an important step for improving the quality of sperm after collection in aquatic animals (Ciereszko et al, 2000;Bobe and Labbe, 2009).…”

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confidence: 99%

“…The osmolality of seminal plasma was determined using an Osmometer 3000 (Gonotech, Berlin Germany). Four different extenders such as ringer solution for marine fish (RSMF, Chao et al, 1973), modified marine fish ringer solution (MMFR, Kurokura et al, 1984), V2E (Stein and Bayrle, 1976) and honey in fish ringer solution (HFRS, Hara et al, 1982) were selected. All the extenders were prepared fresh using double distilled water.…”

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confidence: 99%

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Assessment of sperm quality and short term preservation of sperm in grey mullet Mugil cephalus Linnaeus, 1758

Balamurugan

Munuswamy

2016

Indian J. Fish.

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Quality of fresh milt of grey mullet Mugil cephalus Linnaeus, 1758 was assessed based on motility grade, motility duration, sperm count and seminal plasma osmolality. Freshly collected milt from live males (n=17) were examined individually for motility assessment. Motility grade was four in fifteen samples whereas three in other two samples analysed. Other sperm quality parameters such as motility duration, sperm density and seminal plasma osmolality were : 421.38±137 sec, 1.67±0.3x10 8 nos. ml -1 and 362.28±79.13 m Osmol kg -1 respectively. To standardise short term preservation of sperm, four different extenders such as ringer solution for marine fish, modified marine fish ringer solution, V2E extender and honey in fish ringer solution were experimented. The results indicated that short term preservation at 4 o C over a period of 10 days with V2E extender at 1:1 ratio significantly (p<0.05) retained the highest sperm motility grade (2.6±0.5) over other dilutions such as 1:2 and 1:3 on 5 th day. However, on 10 th day of observation, sperms were immotile after activation with seawater, in undiluted as well as diluted milt samples. This probably indicates that V2E extender is effective for short term preservation of sperm in grey mullet.

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confidence: 99%

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confidence: 99%

Assessment of sperm quality and short term preservation of sperm in grey mullet Mugil cephalus Linnaeus, 1758

Balamurugan

Munuswamy

2016

Indian J. Fish.

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“…Squeezing the abdomen of males, semen was collected in haematocrit tube (Terumo Co., Tokyo, Japan) and semen volume was measured. Then, the collected semen was immediately mixed with 50 μL of immobilizing solution (IS) (128.4 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl2, 2.4 mM NaHCO3: Kurokura et al, 1984) in 0.2 mL volume microtubes. Subsequently, the diluted semen was stored at 4°C.…”

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confidence: 99%

Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization ofMisgurnus anguillicaudatusfemale withM. mizolepismale

Fujimoto

Yasui

Yoshikawa

et al. 2008

Journal of Applied Ichthyology

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Short title: Diploid and triploid Misgurnus hybrid males SummaryDiploid and triploid interspecific hybrid male progeny obtained from mating Misgurnus anguillicaudatus with M. mizoleis were reported to have histologically fertile and sterile testes, respectively. However, their reproductive capacity is still unclear because mating tests have not been examined using mature hybrids. Here, we examined physiological and genetic characteristics of spermatozoa of diploid and triploid hybrids. In diploid hybrid males, 1n, 2n and 4n spermatozoa showing low motility were detected. However, spermatozoa of three diploid hybrid males could generate 2n larvae. Therefore, only 1n spermatozoa of diploid hybrid males was fertile to produce larva. The chromosomes of diploid hybrid males were transmitted to spermatozoa by random segregation between the homologous chromosomes because most larvae had one allele derived from both M. anguillicaudatus and M. mizolepis at all loci examined. In triploid hybrid males, spermatozoa could be categorized to three different types based on their ploidy status. Type 1: In the first and second males, sperm samples mainly comprised 6n spermatozoa. Motility and fertility were not recorded. Type 2: The third male gave a large proportion of 6n spermatozoa as well as a small proportion of 1n spermatozoa. Although no motility was observed, larvae arose from eggs inseminated with such spermatozoa. Type 3: In the fourth male, only 1n spermatozoa were detected and their motility was vigorous. When eggs were fertilized with such 1n spermatozoa, normal larvae hatched. 1n spermatozoa of the triploid hybrid male only included the M. anguillicaudatus genome. In Misgurnus fishes, diploid hybrid males exhibited semi-sterility or slight fertility. On the contrary, triploid hybrid males were sometime fertile due to the production of 1n spermatozoa by a kind of transformation of meiosis like meiotic hybridogenesis. Keywords: Misgurnus, hybrid male, sterility, triploid, spermatozoa IntroductionDiploid hybrids between two different fish species exhibit gonadal, gametic, or zygotic sterility depending on the combination of species (Chevassus, 1983). However, fertile hybrid fish often show alternative atypical reproduction, such as unreduced gametogenesis, gynogenesis, or hybridogenesis, especially in females (reviewed by Dawley, 1989). On the contrary, hybrid males are usually sterile in most cases, but a reproduction with fertile unreduced sperm was reported in the Iberian minnow with a natural hybrid origin (Alves et al., 1999) and common carp × crucian carp hybrids (Cherfas et al 1994; Liu et al., 2001;Sun et al., 2007).Interspecific hybridization has been regarded as a useful tool for aquaculture to improve growth rate as well as to add desirable traits (Bartley et al., 2001). Moreover, hybridization could be used as a means to prevent biological contamination, because sterile hybrid fishes are not risky to contaminate an indigenous gene pool by escaping from cages to natural environment (Bartley et al., 2001). Ho...

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“…Ringer solution -128 mM NaCl, 2.7 mM KCl, 1.4 mM CaCl 2 , 2.4 mM NaHCO 3 (Kurokura et al, 1984) Methanol + DMA (70:30) 8% 1:6 Unpublished data…”

Section: Gariepinusmentioning

confidence: 99%

Sperm Cryopreservation of Some Freshwater Fish Species in Malaysia

Chew¹,

Zulkafli²

2012

Current Frontiers in Cryopreservation

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Cryopreservation of carp sperm

Cited by 140 publications

References 15 publications

Assessment of sperm quality and short term preservation of sperm in grey mullet Mugil cephalus Linnaeus, 1758

Assessment of sperm quality and short term preservation of sperm in grey mullet Mugil cephalus Linnaeus, 1758

Genetic and reproductive potential of spermatozoa of diploid and triploid males obtained from interspecific hybridization ofMisgurnus anguillicaudatusfemale withM. mizolepismale

Sperm Cryopreservation of Some Freshwater Fish Species in Malaysia

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