Cryopreservation of chestnut by vitrification of in vitro-grown shoot tips

Vidal, Nieves; Sánchez, Conchi; Jorquera, Lorena; Ballester, A.; Viéitez, A. M.

doi:10.1079/ivp2004596

Cited by 30 publications

(26 citation statements)

References 17 publications

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“…The cold storage of cultures represents a procedure for medium-term conservation, and Janeiro et al ( 1995 ) reported the possibility of keeping chestnut cultures at 2-4°C for up to 1 year without subculture. In addition, a successful cryopreservation system of chestnut shoot tips has been reported (Vidal et al 2005 ) allowing long-term storage of chestnut genotypes in liquid nitrogen. A detailed protocol for micropropagation of European chestnut, including storage and molecular marker analysis to determine the genetic stability of in vitro regenerated plants, has recently been published ) .…”

Section: Propagationmentioning

confidence: 99%

Chestnut

et al. 2011

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The genus Castanea , chestnuts and chinkapins, belongs to the family Fagaceae , which includes other important timber producing genera such as Quercus and Fagus . The genus Castanea is divided into three geographically delimited sections with at least seven consistently recognized interfertile species: 4 species in Asia ( C. mollissima , C. henryi , C. seguinii , and C. crenata ), two or more species in North America ( C. dentata , C. ozarkensis , and C. pumila ) and one in Europe and Turkey ( C. sativa ). The two most important diseases of chestnut are ink disease ( Phytophthora ) and chestnut blight ( Cryphonectria ). Resistance to these is the major objective for rootstock breeding in Europe and scion breeding in North America. In both cases, the source of resistance was Asian species. European breeding programs developed resistant hybrid rootstocks, which are propagated by stooling, cuttings, or in vitro culture. A major pest of chestnut is the gall wasp Dryocosmus kuriphilus whose control is based on the spread of parasitoids but also on the selection of resistant cultivars. For nut production, the most important breeding objectives include the following: good horticultural traits, product quality, suitability to storage and processing, and ease of peeling. For timber, important characters include wood quality, rapid growth, and nonchecking of wood (ring-shake). Molecular maps have been developed, which has expanded the genetic knowledge of the chestnut. An effi cient genetic transformation protocol for C. sativa through the coculture of somatic embryos with different strains of Agrobacterium tumefaciens has been described.

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Section: Propagationmentioning

confidence: 99%

Chestnut

et al. 2011

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“…The vitrification-based procedure described by Vidal et al (2005) was applied to 46 genotypes (Table 1) of the 130 genotypes established in vitro. The number of shoot apices cryopreserved per genotype varied from 90 to 300, and was related to the proliferation capacity of each genotype.…”

Section: Cryopreservation Of Chestnut Shoot Tipsmentioning

confidence: 99%

“…Finally, the cryopreservation of embryogenic cultures from mature cork oak material was also found to be feasible, with high levels of embryo recovery recorded (88-93%) (Valladares et al 2004). Cryopreservation of chestnut shoot apices from in vitro cultures multiplied through axillary shoots was also reported as an alternative to preservation of zygotic embryo axes and somatic embryos (Vidal et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Establishment of cryopreserved gene banks of European chestnut and cork oak

Vidal

Viéitez

Fernández³

et al. 2010

Eur J Forest Res

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Cryopreservation of selected genotypes of European chestnut and cork oak was carried out in two laboratories in a project involving conservation of field collections. Plant material was selected on the basis of disease resistance (chestnut), growth habit, phytosanitary performance and cork quality (cork oak). The cryopreservation technique comprised of vitrification of shoot apices isolated from in vitro stock shoot cultures (chestnut) and somatic embryos (cork oak). Forty-three out of 46 chestnut genotypes assayed survived the freezing process, but only 63% recovered their capacity to produce new shoots. After completion of multiplication and rooting steps, the surviving shoots produced plants that were morphologically identical to those derived from non-supercooled material. All 51 cork oak genotypes withstood freezing and were able to produce new somatic embryos through a process of secondary embryogenesis. Multiplication and germination of the recovered embryos enabled production of plants that were morphologically identical to those derived from nonsupercooled material. In light of the results obtained, longterm cryopreservation of these species is feasible, thereby ensuring conservation of valuable genotypes during field evaluation.

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“…In C. sativa, Vidal et al [115] and Jorquera et al [116] investigated the possibility of cryopreserving shoot tips from material of juvenile and adult origin excised from in vitro cultures using the vitrification process. The shoot recovery percentages varied between 33-54% depending on the genotype, although these differences did not appear to be related to the juvenile or adult status of the material.…”

Section: Long-term Preservation (Cryopreservation)mentioning

confidence: 99%

Application of Biotechnology in the Conservation of the Genus Castanea

Corredoira

Martínez

Cernadas

et al. 2017

Forests

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Castanea is a hardwood forest genus of considerable agro-economic importance for both timber and nut production. Chestnuts are one of the most significant nut crops in the temperate zone. However, this species is threatened by pollution, social factors, economical changes, and two major fungal diseases: ink disease (Phytophthora spp.), and chestnut blight canker (Cryphonectria parasitica). Similar to other wood species, chestnuts are difficult to propagate both generatively by seed and vegetatively by means of grafting or cuttings. Biotechnological methods such as in vitro culture have been developed in the last few years as an alternative to conventional vegetative propagation. Biotechnology plays a very important role not only in the propagation of selected individuals (being used at a commercial level), but also in its short-term preservation, and offers the possibility of preserving the propagated material in the medium-term (cold storage) or long-term using cryopreservation.

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Cryopreservation of chestnut by vitrification of in vitro-grown shoot tips

Cited by 30 publications

References 17 publications

Chestnut

Chestnut

Establishment of cryopreserved gene banks of European chestnut and cork oak

Application of Biotechnology in the Conservation of the Genus Castanea

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