Cryopreservation of Drosophila melanogaster embryos

Mutation accumulation and the effect of copia insertions in Drosophila melanogaster

“…All of our samples were stored in a single Dewar flask, which might have suffered from an event such as depletion of liquid nitrogen at some point during storage. In previous work, rates of success in reviving embryos of a particular test genotype were as high as 45 % (Steponkus & Caldwell, 1993). Our results (here and Houle et al, 1997) are the only attempts we know of to revive embryos from cryopreservation after more than a few days.…”

Overcoming a Permeability Barrier by Microinjecting Cryoprotectants into Zebrafish Embryos (Brachydanio rerio)

“…Additionally, vitrification may be more suitable for multicellular organisms because the freezing protocol does not have to be optimized by cell type, as is the case in slow freezing (9). All of these issues were faced in attempting to cryopreserve the Drosophila embryo {Drosophila melanogaster) and were overcome by vitrification (21,28,29). Although vitrification has many advantages, selection of a vitrification solution is difficult, because the highly concentrated solutions have undesired toxic or teratogenic effects (6,7,9).…”

The Problem of Contamination: Open vs. Closed vs. Semi-Closed Vitrification Systems

“…The extremely radiation-resistant bacterium Deinococcus radiodurans is inactivated (>-4log) by administe-ring 400.000 µWs/cm² per each sterilization cycle. An adequate amount of UV radiation deactivates the growth of all kinds of micro-organisms, from viruses like Hepatitis (which require an 8.000 UV dose) to fungi like Aspergillus Niger (330.000 UV dose) (33). At CRYO 2011 Dr. Parmegiani spoke about a new dispositive of UV sterilization of the common containers with cells or tissues inside but the scientific community thinks that is too dangerous biological samples exposed to UV rays without any protection.…”