Cryopreservation of Embryos and Gametes: Past, Present, and Future

Szeptycki, J.; Bentov, Yaakov

doi:10.5772/65123

Cited by 3 publications

(1 citation statement)

References 132 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is important to note that the present methodology aimed only at post-thawed samples and fresh ejaculates were not supported. Though fresh spermatozoa have similar performance than the frozen counterpart (Szeptycki and Bentov, 2016), their captures under microscope and kinetics are different.…”

Section: Discussionmentioning

confidence: 99%

Objective assessment of bull sperm motility parameters using computer vision algorithms

RodrÃguez-MontaÃ±a¹,

Roa-Guerrero²

2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

The quality control of frozen semen samples from cattle is established by parameters such as percentage of progressive motility (% MP) because it is related to the fertilization capacity of bulls. Nowadays, sperm quality test is performed by direct visual inspection of sperm through a microscope in andrology laboratories. However, there is a high subjectivity in the observation and assessment depending on the observer; thus, causing unreliable diagnoses and non-repetitiveness in results. The development of a low cost computer tool was proposed to identify the individual sperm motility in cattle through the application of artificial vision algorithms. The methodology consisted of: the acquisition and pre-processing of videos obtained from thawed cryopreserved samples, the segmentation, filtering and detection of spermatozoa using the Fisher Discriminant Analysis and Adaptive Gaussian Models, followed by the assignment and construction of sperm trajectories through the Munkres Algorithm and Kalman Filter. Finally, the characterization and assessment of sperm motility parameters were performed based on the criteria present in computer-aided semen analysis (CASA) commercial systems. The results obtained showed high correlation for the individual sperm motility with a determination coefficient of 0.8143 for 10 different samples of bull sperm with respect to manual analysis. Likewise, a concordance coefficient of 0.966 was found in the 95% confidence interval using the Bland-Altman test, indicating that the measures were highly similar. In this way, the methodology is a reliable technological support that contributes to the improvement of quality control of semen samples from cattle.

show abstract

Section: Discussionmentioning

confidence: 99%

Objective assessment of bull sperm motility parameters using computer vision algorithms

RodrÃguez-MontaÃ±a¹,

Roa-Guerrero²

2017

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

Cryopreservation and Biobanking of Gametes, Embryos, and Reproductive Tissues

Gao,

Ma,

Ren

et al. 2024

Biopreservation and Biobanking

View full text Add to dashboard Cite

Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

Anisa-Annur,

Wan-Hafizah,

Nor-Ashikin

et al. 2024

Asian Pacific Journal of Reproduction

View full text Add to dashboard Cite

Objective: To investigate the effects of coenzyme Q10 (CoQ10) supplementation on post-vitrification embryo development and gross morphology. Methods: Balb/c mouse embryos were cultured in potassium simplex optimised medium (KSOM) with varying CoQ10 concentrations [0 (control), 20, 40, and 60 μΜ]. The most effective CoQ10 concentration (40 μM) was selected for subsequent post-vitrification morphology study. Embryos were randomly divided into four groups: Group A (non-vitrified without CoQ10), Group B (non-vitrified with CoQ10), Group C (vitrified without CoQ10), and Group D (vitrified with CoQ10), followed by vitrification at the 8-cell stage. Survival rates and development until the blastocyst stage were evaluated through morphological examinations using ASEBIR's system, distinguishing normal and abnormal embryos. Results: Supplementation of 40 μM CoQ10 significantly increased blastocyst formation (95%) compared to the control group (92%), 20 μM (62%), and 60 μM (56%) (P<0.001). Following vitrification, Group D exhibited a significant increase in blastocyst formation (92%) compared to Group C (82%) (P<0.05). Morphological assessments indicated superior embryo quality in Group B over Group D during the cleavage stage, morula, and blastocyst (P<0.05). Conclusions: CoQ10 supplementation exhibits promising potential to enhance preimplantation embryo development, increase blastocyst formation rates, and improve embryo quality post-vitrification. This offers a promising approach to mitigate oxidative stress on embryos, potentially improving overall assisted reproductive technology outcomes.

show abstract

Cryopreservation of Embryos and Gametes: Past, Present, and Future

Cited by 3 publications

References 132 publications

Objective assessment of bull sperm motility parameters using computer vision algorithms

Objective assessment of bull sperm motility parameters using computer vision algorithms

Cryopreservation and Biobanking of Gametes, Embryos, and Reproductive Tissues

Effect of coenzyme Q10 supplementation on post-vitrification mouse embryo development

Contact Info

Product

Resources

About