Cryopreservation of embryos of humpback grouper (Cromileptes altivelis) using combinations of non-permeating cryoprotectants

The effect of extender on the quality of climbing perch ( Anabas testudineus ) sperm after refrigerated storage

“…In cryopreservation (−179°C), glucose is frequently added in extenders as a non‐permeable cryoprotectant (Muchlisin, 2005; Yamaner et al, 2015), such as in the cryopreservation of sperm obtained from Cromileptes altivelis (Li et al, 2022), Glossogobius giuris (Grace, 2021) and Mystus mumurus (Muchlisin et al, 2004). Therefore, the glucose base serves not only as an extender but also as a cryoprotective agent in the cryopreservation of sperm.…”

“…Furthermore, glucose-based solutions have been reported to be suitable for preserving the sperm of Barbonymus gonionotus fish at temperatures ranging from 2 to −34°C, as well as preserving the quality of climbing perch sperm (Abinawanto et al, 2013;Hossen et al, 2017). According to Şahin In cryopreservation (−179°C), glucose is frequently added in extenders as a non-permeable cryoprotectant (Muchlisin, 2005;Yamaner et al, 2015), such as in the cryopreservation of sperm obtained from Cromileptes altivelis (Li et al, 2022), Glossogobius giuris (Grace, 2021) and Mystus mumurus (Muchlisin et al, 2004).…”

Comparison of Sucrose and Trehalose for Protein Stabilization Using Differential Scanning Calorimetry

“…15 These two disaccharides possess the ability to stabilize biological materials at cryogenic temperatures, thereby preventing structural damage to biomolecules. 16,17 The primary function of cryoprotectants is to prevent the formation of ice crystals, instead creating a glassy-like matrix that surrounds and stabilizes the molecules. 16 The temperature at which molecular motion rapidly slows down, increasing the viscosity of a material to 10 12 Pa s, is referred to as the glass transition temperature, T g .…”

“… 15 These two disaccharides possess the ability to stabilize biological materials at cryogenic temperatures, thereby preventing structural damage to biomolecules. 16 , 17 …”

“…Commonly used permeating cryoprotectants can induce toxicity when large quantities cross the cell membrane. − The disaccharides sucrose and trehalose are possible replacements or complements to permeating cryoprotectants . These two disaccharides possess the ability to stabilize biological materials at cryogenic temperatures, thereby preventing structural damage to biomolecules. , …”