Cryopreservation of Fish Gametes: An Overview

Jawahar, K. Thilak Pon; Betsy, Judith

doi:10.1007/978-981-15-4025-7_7

Cited by 9 publications

(9 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most reliable measure for cryopreservation success is the fertilizing capability of cryopreserved milt (Jawahar & Betsy, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…The most reliable measure for cryopreservation success is the fertilizing capability of cryopreserved milt (Jawahar & Betsy, 2020). As a thumb rule, a strong correlation is reported in post‐thaw motility and fertilizing capability of fish sperm (Ciereszko et al, 2014); however, better post‐thaw motility is not always decisive for successful fertilization for sperm (Akcay et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Methanol proved to be a good cryoprotectant, yet being a low molecular weight compound it penetrates rapidly inside the cell (Horvath et al, 2005) and the cell is deprived of some external cryoprotectant. It is reported that a combination of external and internal cryoprotectants is most promising for cryo‐survivability of sperm compared to any individual class (Jawahar & Betsy, 2020). A combination of methanol, a permeating cryoprotectant and CPLL a non‐permeating cryoprotectant in the present study provides sperm best milieu for fertilization.…”

Section: Discussionmentioning

confidence: 99%

“…Cryoprotectants are used to protect the sperm which alter the size and shape of ice crystals, bind with water thus preventing dehydration and crystal formation and interact with the membranes in a way that overcomes their brittleness (Jawahar & Betsy, 2020). Among cryoprotectants, permeating ones (DMSO, methanol, propylene glycol and glycerol) are low molecular weight molecules which permeate the cell membrane and inhibit the diffusion of water from the cell to extracellular ice crystals.…”

Section: Introductionmentioning

confidence: 99%

“…The non‐permeating cryoprotectants include high molecular weight molecules that protect the cells by depressing the freezing point and raising the glass transformation temperature of the extracellular solution. Sucrose, glucose, dextran, egg yolk serum, skim milk and anti‐freeze agents are in this category (Jawahar & Betsy, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Evaluation of carboxylated poly‐l‐lysine for cryopreservation of Labeo rohita sperm

Bibi

Awan

Rakha

et al. 2023

Reprod Domestic Animals

View full text Add to dashboard Cite

Carboxylated poly‐l‐lysine (CPLL) is an anti‐freeze agent having pronounced non‐permeating yet membrane stabilizing cryoprotective capabilities. The objective was to evaluate the CPLL supplementation in extender in terms of post‐thaw quality (sperm), total anti‐oxidant activity (milt) and fertilization potential of cryopreserved Labeo rohita sperm. For this purpose, male brood fish reared at a fish seed hatchery, Rawal Town Islamabad, Pakistan were captured from different rearing ponds and acclimatized in hatchery ponds for 6 h. The brooder was injected with Ovaprim (0.2 mL/kg), and milt was collected after 8 h in cooled sterilized falcon tubes, maintained at 4°C and evaluated for sperm motility. The milt collected from three brooders (n = 3) was diluted in extenders viz., modified Kurokura‐2 extender having 10% methanol (control); experimental extenders with CPLL supplementation at the rate of 0.5%, 1% and 1.5%. Diluted milt was filled in 0.5 mL straws, exposed to liquid nitrogen vapours and cryopreserved. Cryopreserved milt was thawed at 25°C and assessed for post‐thaw sperm quality. Sperm motility, motility duration, viability, total anti‐oxidant capacity and DNA integrity was significantly higher (p < 0.05) in the extender having 1.5% CPLL than control. To evaluate the fertilization rates, male and female brooders were injected with Ovaprim at 0.2 mL/Kg and 0.5 mL/Kg body weight respectively. Fresh eggs and milt were collected through abdominal stripping. Batches of 10 g of eggs from each female (n = 2) were fertilized with one straw, each from frozen sperm with KE + methanol (control), KE + methanol + 1.5% CPLL and 50 μL fresh milt (negative control). After 1.5 h of fertilization, eggs were collected from all jars and a total of 200 eggs were counted. The fertilized eggs appeared clear and transparent while unfertilized eggs looked opaque with disintegrated nuclei. Sperm fertilization rate (%) was higher (p < 0.05) in extender KE + methanol + 1.5% CPLL (78.7 ± 0.5) compared to control (KE + methanol) (52.0 ± 0.4) however, it was lower compared to that of negative control, the fresh milt (85.2 ± 0.6). In conclusion, supplementation of carboxylated poly‐l‐lysine (1.5%) to modified Kurokura‐2 extender having 10% methanol improves post‐thaw motility, motility duration, viability, DNA integrity, anti‐oxidant capacity (milt) and fertilizing ability of cryopreserved L. rohita sperm.

show abstract

“…The most reliable measure for cryopreservation success is the fertilizing capability of cryopreserved milt (Jawahar & Betsy, 2020).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%