Cryopreservation of human brain tissue

Robbins, Richard J.; Torres‐Alemán, Ignacio; Léránth, Csaba; Bradberry, Charles W.; Deutch, Ariel Y.; Welsh, Susan; Roth, Robert H.; Spencer, Dennis D.; Redmond, D. Eugene; Naftolin, Frederick

doi:10.1016/0014-4886(90)90137-h

Cited by 43 publications

(6 citation statements)

References 16 publications

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“…Maintenance of living brain tissue and brain organoids after cryopreservation is challenging due to the complex cytoarchitecture and neural connectivity. 13 , 23 Here, we developed MEDY cryopreservation, which can be applied to multiple neural organoids including the dorsal and ventral forebrain, SP organoids, OVB organoids, epilepsy patient-derived brain organoids, and even human living brain tissues. MEDY cryopreservation could protect the organoid structure and synaptic function and inhibit the endoplasmic reticulum-mediated cell apoptosis pathway.…”

Section: Discussionmentioning

confidence: 99%

“… 12 However, with limited accessibility and manipulability, cryopreservation and reconstruction of living brain tissue with specific pathological features remain hugely challenging, as it is hard to maintain the survival of large amounts of functional neurons. 13 , 14 Therefore, it is imperative to develop reliable cryopreservation technology for fresh viable human brain tissue and for brain organoids, which can be used to study the pathological mechanisms of brain disease, organoid transplantation for brain injury, and/or drug discovery.…”

Section: Introductionmentioning

confidence: 99%

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Effective cryopreservation of human brain tissue and neural organoids

Xue,

Li,

et al. 2024

Cell Reports Methods

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effective cryopreservation of human brain tissue and neural organoids

Xue,

Li,

et al. 2024

Cell Reports Methods

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“…Previous attempts to cryopreserve VM cells were made using either tissue pieces or single cells suspensions (Collier et al, 1988; Robbins et al, 1990; Sauer et al, 1992; Dong et al, 1993; Sautter et al, 1996; Koopmans et al, 2001). We chose to cryopreserve a single cell suspension so that a more complete battery of tests could be performed on an aliquot of the actual material in cryostorage.…”

Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of human or rat ventral mesencephalon has been examined for many years, with mixed results (Collier et al, 1988, 1993; Robbins et al, 1990; Sauer et al, 1992; Dong et al, 1993; Sautter et al, 1996). Postthaw cell viabilities have been reported from 35% to 99%, and cell recoveries range from 25% to 82%.…”

mentioning

confidence: 99%

Comparison of fresh and cryopreserved porcine ventral mesencephalon cells transplanted in A rat model of Parkinson's disease

Jacoby¹,

Lindberg²,

Ratliff³

et al. 2002

J of Neuroscience Research

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To evaluate whether cryopreservation of porcine ventral mesencephalon cells influences graft survival and function in vivo, we have transplanted either freshly prepared or cryopreserved cells into the striatum of 6-hydroxydopamine-lesioned rats. A single cell suspension of porcine ventral mesencephalon cells from the same isolation either was stored at 4 degrees C and transplanted the next day or was cryopreserved for 4 weeks in liquid nitrogen vapor. The cryopreserved cells were then rapidly thawed, rinsed, and transplanted in the same manner as the fresh cells, with the same dose of viable cells. All animals received daily injections of cyclosporin A to prevent xenograft rejection. To monitor graft function, amphetamine-induced rotation was measured every 3 weeks between 6 and 15 weeks posttransplantation. After sacrifice at 15 weeks posttransplantation, histological methods were used to compare fresh cell and cryopreserved cell transplants with respect to graft survival, differentiation and integration, and host immune response. Cryopreserved cells were found to be either equivalent or in some cases superior to fresh cells with respect to rotational correction, graft survival, graft volume, numbers of graft-derived dopaminergic neurons, and host immune responses. In conclusion, the results indicate that it is feasible to cryopreserve porcine ventral mesencephalon cells for long-term storage of cells prior to transplantation in an animal model of Parkinson's disease.

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“…Ex vivo studies have demonstrated that this cryopreservation temperature can adequately preserve neuronal cells viability and specific features [22,23].…”

Section: Preparation Of Meningeal Membranesmentioning

confidence: 98%