Cryopreservation of Human Spermatozoa by Vitrification vs. Slow Freezing: Canadian Experience

Moskovtsev, Sergey I.; Lulat, A.G-M.; Librach, Clifford

doi:10.5772/35485

Cited by 18 publications

(20 citation statements)

References 86 publications

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“…Moskovtsev group also reported excellent recovery in terms of sperm kinematic parameters and DNA fragmentation in a study on 11 infertile men, where they compared slow freezing vs. vitrification of sperm [37]. An interesting finding in this study though, was that although sperm kinematic parameters were found to be better, sperm DNA fragmentation index did not differ between the two groups.…”

Section: Sperm Vitrificationsupporting

confidence: 52%

Human Sperm Freezing: Mini Update

Dupesh¹,

Rasappan²,

Shila³

et al. 2018

ARSci

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Sperm freezing is widely used in ART clinics around the globe. Very little has actually changed with respect to cryopreservation protocols and methodology of freezing over the last 50 years. The aim of this paper is to briefly review the basic principles that underlie freezing and ice crystal formation and also provide a brief overview of newer sperm freezing techniques like sperm vitrification and freeze drying of sperm.

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Section: Sperm Vitrificationsupporting

confidence: 52%

Human Sperm Freezing: Mini Update

Dupesh¹,

Rasappan²,

Shila³

et al. 2018

ARSci

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“…Here we want to emphasize that despite of skepticism, denial, or even open hostility towards publications and presentations faced by the Isachenkos (and by Katkov as their strong proponent), the method had matured into a technology, which proved to be robust and feasible for ART practitioners as well brought food for thoughts to those who works in the realm of basic cryobiology. Most importantly, the results led to the birth of healthy babies and at least one group has repeated the Isachenko method and has obtained good results completely independently-they report their data in Chapter 3 [Moskovtsev et al, 2012]. The authors dedicate a separate Chapter 2 in this Book for summarizing their achievements [Isachenko et al, 2012].…”

Section: Resurrection and Rise Of Kinetic Vitrification Of Sperm: Thementioning

confidence: 99%

Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature?

Katkov¹,

Bolyukh²,

Chernetsov³

et al. 2012

Current Frontiers in Cryobiology

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“…Various methods of freezing spermatozoa have been previously described1016171819202122 mostly using slow-cooling and different cryoprotectants 23. However, despite the absence of clear successful vitrification outcomes in the mouse, successful vitrification of human,122425262728293031 canine,32 and fish ( Oncorhynchus mykiss )33 spermatozoa has been reported using specific capillaries, low sample volume (3 μl), dropped directly into liquid nitrogen, and high cooling rates.…”

Section: Discussionmentioning

confidence: 99%

“…An important factor affecting vitrification is the type of carrier used 23. As a novel approach, we loaded samples on to fiber plugs (Cryologic®) to provide the highest possible cooling rates using 3 μl volumes and no straw barriers.…”

Section: Discussionmentioning

confidence: 99%

Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice

et al. 2017

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This study compared three cryopreservation protocols on sperm functions, IVF outcomes, and embryo development. Epididymal spermatozoa cryopreserved using slow-cooling (18% w/v raffinose, RS-C) were compared with spermatozoa vitrified using 0.25 M sucrose (SV) or 18% w/v raffinose (RV). The motility, vitality, and DNA damage (TUNEL assay) of fresh control (FC) spermatozoa were compared with post-thawed or warmed RS-C, RV, and SV samples. Mouse oocytes (n = 267) were randomly assigned into three groups for insemination: RV (n = 102), RS-C (n = 86), and FC (n = 79). The number and the proportion of two-cell embryos and blastocysts from each treatment were assessed. Sperm motility (P < 0.01) and vitality (P < 0.05) were significantly reduced after vitrification compared with slow-cooled spermatozoa. However, DNA fragmentation was significantly reduced in spermatozoa vitrified using sucrose (15 ± 1.8% [SV] vs 26 ± 2.8% [RV] and 27 ± 1.2% [RS-C]; P < 0.01). Although the number of two-cell embryos produced by RS-C, RV, and FC spermatozoa was not significantly different, the number of blastocysts produced from two-cell embryos using RV spermatozoa was significantly higher than FC spermatozoa (P = 0.0053). This simple, small volume vitrification protocol and standard insemination method allows successful embryo production from small numbers of epididymal spermatozoa and may be applied clinically to circumvent the need for ICSI, which has the disadvantage of bypassing sperm selection.

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Cryopreservation of Human Spermatozoa by Vitrification vs. Slow Freezing: Canadian Experience

Cited by 18 publications

References 86 publications

Human Sperm Freezing: Mini Update

Human Sperm Freezing: Mini Update

Kinetic Vitrification of Spermatozoa of Vertebrates: What Can We Learn from Nature?

Minimal volume vitrification of epididymal spermatozoa results in successful in vitro fertilization and embryo development in mice

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