Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification

Gupta, Mukesh Kumar; Uhm, Sang Jun; Lee, Hoon Taek

doi:10.1016/j.theriogenology.2006.07.015

Cited by 104 publications

(82 citation statements)

References 52 publications

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“…Also the number and structure of lipid droplets in different species can cause different cryosensitivity injuries for oocytes [38]. Some researchers deplete lipid droplets from the oocyte cytoplasm before cryopreservation [35]. However, without manipulation of lipid droplets, successful results have been acquired in the present study after vitrification and warming.…”

Section: Discussionmentioning

confidence: 71%

“…Presence of lipid droplets and vacuoles in the ooplasm of sheep oocytes is an important obstacle in the way of oocyte cryopreservation; lipid droplets make oocytes more sensitive to cooling, causing irreversible injuries to membrane structures at low temperatures [35][36][37]. Also the number and structure of lipid droplets in different species can cause different cryosensitivity injuries for oocytes [38].…”

Section: Discussionmentioning

confidence: 99%

“…In the present research, we considered previous reports [31,35] and used the combination of EG and DMSO for COC vitrification. As expected, good survival of COCs was obtained after vitrification.…”

Section: Discussionmentioning

confidence: 99%

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In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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Purpose The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities. Methods Freshly isolated (control group) and vitrifiedwarmed COCs (cryotop group) were matured in vitro. The expression of pro-and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis. Results The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25±3.01% and 51.94±2.7% respectively. The expression rates of proand anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p<0.05). Conclusion Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.

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Section: Discussionmentioning

confidence: 71%

Section: Discussionmentioning

confidence: 99%

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In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Ebrahimi

Valojerdi

Eftekhari‐Yazdi

et al. 2010

J Assist Reprod Genet

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“…Recently new vitrification techniques have been applied for cryopreservation of porcine oocytes (Jain and Paulson, 2006;Gupta et al, 2007;Huang et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Varga

Gajdócsi

Makkosné

et al. 2008

Acta Veterinaria Hungarica

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The breeding of Mangalica, a native pig breed in Hungary, had been started in 1833, but this pig breed almost became extinct in Hungary in the past decades. In 1991, the number of sows was only 200. Although in these days the existing Mangalica population consists of more than 6000 animals representing different colour variations, the preservation of this traditional pig breed is still very important. Vitrification is a potential tool for the preservation of gametes and embryos of these animals. The aim of this study was to investigate the effects of vitrification on the developmental competence of Mangalica (M) and Large White (LW) oocytes following fertilisation. The oocytes were vitrified by the Open Pulled Straw (OPS) method using different concentrations of ethylene glycol and dimethyl sulphoxide as cryoprotectants. After rehydration the oocytes underwent in vitro fertilisation; the resultant zygotes were then cultured in vitro for four days to assess embryonic development. In the first experiment, in vitro maturation of M and LW oocytes was compared. No significant difference was observed in the nuclear maturation rate of LW and M oocytes. In the second experiment, the sensitivity of oocytes to vitrification was examined by evaluating oocyte morphology after thawing. A higher percentage of LW oocytes showed normal morphology compared to M oocytes, indicating that Mangalica oocytes are more sensitive to cryoprotectants than Large White oocytes. After warming and in vitro fertilisation, more than 50% of the oocytes started embryonic development and by the end of the incubation period morula stage embryos had developed in both groups. The results show that the OPS vitrification technique is well suited to preserve Mangalica oocytes and from these oocytes morula embryos can be produced.

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“…Oocytes can be cryopreserved using slow cooling or vitrification. Vitrification is an alternative to traditional freezing methods (slow freezing) to avoid chilling injury and ice crystal formation [2,3]. Kuleshova et al reported the first birth from vitrified human oocytes [4].…”

Section: Introductionmentioning

confidence: 99%

Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes

Khosravi-Farsani

Sobhani

Amidi

et al. 2010

J Assist Reprod Genet

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Purpose Evaluation of viability and subsequent developmental ability of mouse germinal vesicle breakdown oocytes vitrified in conventional straws. Methods Oocytes with compact cumulus cells were cultured for 3 h in TCM199 medium GVBD and vitrified by two methods: the step-wise and single-step. After vitrification, the oocytes were thawed, and subjected to in vitro maturation and in vitro fertilization. Oocyte survival (postthaw) was assessed by morphological appearance and staining, using propidium iodide (PI)/Hoechst 33342. The oocyte maturation and fertilization rates were examined in vitro. Results In the single-step method the rates of post thaw survival, maturation to metaphase II and cleavage (2-cell embryos) were 58.68%, 56.41% and 38.63%, respectively. In the step-wise method, the corresponding rates were 81.75%, 68.59% and 51.80%, respectively. Conclusion Vitrification of mouse germinal vesicle breakdown oocytes by the step-wise method had the advantage of maintaining the viability and subsequent production of 2-cell embryos. In comparison with that in unvitrified control oocytes, the development of MII oocytes to 2-cell embryos was impaired following vitrification.

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Cryopreservation of immature and in vitro matured porcine oocytes by solid surface vitrification

Cited by 104 publications

References 52 publications

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Vitrification of in vitro matured oocytes of Mangalica and Large White pigs

Mouse oocyte vitrification: the effects of two methods on maturing germinal vesicle breakdown oocytes

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