Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration

Matsumoto, Takeshi; Sakai, Akira; Yamada, Kazuto

doi:10.1007/bf00231963

Cited by 172 publications

(112 citation statements)

References 11 publications

Supporting

Mentioning

101

Contrasting

Unclassified

Order By: Relevance

“…Preculturing shoot tips on solidified medium with a high sucrose concentration is extremely effective at inducing osmo-tolerance for cryopreservation in many plant species (Engelmann et al 2008;Sakai et al 2008). Similarly, LS treatment is very effective at improving the regrowth rate after cryopreservation and is used for many plant species (Matsumoto et al 1994).…”

Section: Resultsmentioning

confidence: 99%

Cryopreservation of blueberry shoot tips derived from in vitro and current shoots using D cryo-plate technique

Dhungana

Kunitake

Niino

et al. 2017

Plant Biotechnology

View full text Add to dashboard Cite

Cryopreservation is an important tool for long-term storage of plant germplasm that is currently used for plant germplasm storage at many institutes worldwide. Recently, novel cryogenic procedures (V and D cryo-plate methods) have been developed. In this study, the most suitable conditions for preserving blueberry shoot tips derived from in vitro and current shoots using the D cryo-plate method were investigated. The D cryo-plate method has advantages such as higher regrowth after cryopreservation and a more user-friendly process compared with conventional cryogenic methods. The optimum duration of desiccation for regrowth of shoot tips from each shoot type was 1 h. To induce dehydration tolerance for the shoot tips, the effects of two cryoprotection treatments (sucrose preculture and loading solution [LS] treatment) on shoot regrowth after cryopreservation were investigated. The combined effect of both treatments significantly increased percentage regrowth (approximately 90%). No regrowth of shoot tips was attained without the two treatments. Thus, preculture and LS treatment were effective to induce dehydration tolerance for cryopreservation of blueberry shoot tips. The optimized conditions for blueberry shoot tips using the D cryo-plate technique were: preculture with 0.3 M sucrose for 1 day, LS treatment (2 M glycerol +0.4-1.0 M sucrose) for 30 min, and air dehydration for 1 h. This optimized procedure was applied to additional blueberry cultivars shoot tips derived from in vitro shoots (regrowth 46.7-100%) and current shoots (regrowth 17.2-62.7%). Furthermore, in vitro shoot tips were suitable material for the D cryo-plate method in blueberry.

show abstract

Section: Resultsmentioning

confidence: 99%

Cryopreservation of blueberry shoot tips derived from in vitro and current shoots using D cryo-plate technique

Dhungana

Kunitake

Niino

et al. 2017

Plant Biotechnology

View full text Add to dashboard Cite

show abstract

“…Osmoprotection regulates the osmotic dehydration of tissues which increases the growth recovery (Matsumoto et al, 1994). Through osmosis, cryoprotectant replaces vacant spaces available in cells when free moving water particles left the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt

Mubbarakh¹,

Izhar²,

Rajasegar³

et al. 2014

Emir. J. Food Agric

View full text Add to dashboard Cite

This study was carried out to evaluate the encapsulation-dehydration technique on IFEs of Rosa hybrida L. cv. Helmut Schmidt. The survival of IFEs was first assessed based on the effects of four sucrose concentrations (0, 0.25, 0.5 and 0.75 M) at different durations (0, 24 and 48 hours). The IFEs were then encapsulated and osmoprotected on shaker at 110 rpm with various sucrose concentrations (0, 0.1, 0.5 and 0.75 M). Subsequently, encapsulated IFEs were dehydrated under laminar air flow during three different time periods (0, 3 and 6 hours) on oven sterilized 50 g silica gel. The encapsulated IFEs were plunged into liquid nitrogen for a minimum duration of 24 hour. Encapsulated IFEs were thawed at 40ºC for 90 seconds and cultured on fullstrength MS medium supplemented with 3% sucrose for a week in dark, a week in semi-light and an additional 1 week under full light exposure. IFEs were then evaluated for survival by 2,3,5-Triphenyltetrazolium Chloride assay at 490 nm. The best conditions for the encapsulation-dehydration of IFEs of Rosa hybrida L. cv. Helmut Schmidt was obtained when IFEs were precultured in 0.25 M sucrose for 24 hours, osmoprotected in 0.75 M sucrose and dehydrated for 3 hours. Histological analysis showed cryopreserved IFEs with intensely stained nucleus but with severely damaged membranes.

show abstract

“…Panis et al (2005b) showed that regrowth of banana meristems is not influenced by loading solution exposure time (0 -5 hours). Although the precise mechanism of loading is not yet fully understood, it has been proven for different plant species that loading can dramatically enhance the tolerance of isolated meristems to dehydration by the vitrification solution (Matsumoto et al 1994, Takagi et al 1997, Panis 2009.…”

Section: IImentioning

confidence: 99%

Cryopreservation of banana’s cv Grand Naine in vitro rhizomes

Londe

Vendrame

Sanaei

et al. 2017

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes of Musa accuminata (AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.

show abstract

Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration

Cited by 172 publications

References 11 publications

Cryopreservation of blueberry shoot tips derived from in vitro and current shoots using D cryo-plate technique

Cryopreservation of blueberry shoot tips derived from in vitro and current shoots using D cryo-plate technique

Establishment of encapsulation-dehydration technique for in vitro fragmented explants of Rosa hybrida L. cv. Helmut Schmidt

Cryopreservation of banana’s cv Grand Naine in vitro rhizomes

Contact Info

Product

Resources

About